Accumulation of phenylpropanoid derivatives in chitosan-induced cell suspension culture of Cocos nucifera

Chakraborty, Mrityunjoy; Karun, Anitha; Mitra, Adinpunya

doi:10.1016/j.jplph.2008.02.004

Cited by 59 publications

(30 citation statements)

References 40 publications

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“…This conclusion contradicts studies describing MDCA as a competitive inhibitor of 4CL (Knobloch and Hahlbrock, 1977;Chakraborty et al, 2009), with only mild inhibitory activity on C4H (Schalk et al, 1998). However, the suggested inhibition of C4H can be explained by the in planta processing of MDCA to PA, which is a well-known inhibitor of C4H (Schalk et al, 1998).…”

Section: Discussionmentioning

confidence: 56%

“…Later on, MDCA was identified as an efficient chemical inhibitor of 4CL, a key enzyme of the general phenylpropanoid pathway leading toward a broad range of secondary metabolites (Knobloch and Hahlbrock, 1977;Funk and Brodelius, 1992;Hartung et al, 1990;Funk and Brodelius, 1994;Chakraborty et al, 2009). Substantial inhibition of the phenylpropanoid pathway can be lethal for the plant (Bonawitz and Chapple, 2013), potentially explaining the allelochemical properties of MDCA (Bertin et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Phenolic Profiling Supports Inhibition of the Core Phenylpropanoid Pathway by MDCA Previous in vitro assays based on heterologous systems provided evidence that MDCA acts as a competitive inhibitor of 4CL (Knobloch and Hahlbrock, 1977;Chakraborty et al, 2009;Fig. 3A).…”

Section: Mdca Affects Plant Growth and Development In A Dosedependentmentioning

confidence: 91%

“…It was suggested to be an allelochemical based on its inhibitory effect on root and shoot growth of Lepidium sativum (Hartung et al, 1990). Independent studies revealed that MDCA acts as an efficient competitive inhibitor of 4-COUMARATE-CoA LIGASE (4CL), the enzyme converting hydroxycinnamates to their corresponding CoA-esters (Knobloch and Hahlbrock, 1977;Chakraborty et al, 2009). This conversion is an early step in the general phenylpropanoid pathway leading to a wide array of metabolites, including coumarins, stilbenes, salicylic acid, flavonoids, and monolignols (Vogt, 2010).…”

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confidence: 99%

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The allelochemical MDCA inhibits lignification and affects auxin homeostasis

et al. 2016

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The phenylpropanoid 3,4-(methylenedioxy)cinnamic acid (MDCA) is a plant-derived compound first extracted from roots of Asparagus officinalis and further characterized as an allelochemical. Later on, MDCA was identified as an efficient inhibitor of 4-COUMARATE-CoA LIGASE (4CL), a key enzyme of the general phenylpropanoid pathway. By blocking 4CL, MDCA affects the biosynthesis of many important metabolites, which might explain its phytotoxicity. To decipher the molecular basis of the allelochemical activity of MDCA, we evaluated the effect of this compound on Arabidopsis thaliana seedlings. Metabolic profiling revealed that MDCA is converted in planta into piperonylic acid (PA), an inhibitor of CINNAMATE-4-HYDROXYLASE (C4H), the enzyme directly upstream of 4CL. The inhibition of C4H was also reflected in the phenolic profile of MDCA-treated plants. Treatment of in vitro grown plants resulted in an inhibition of primary root growth and a proliferation of lateral and adventitious roots. These observed growth defects were not the consequence of lignin perturbation, but rather the result of disturbing auxin homeostasis. Based on DII-VENUS quantification and direct measurement of cellular auxin transport, we concluded that MDCA disturbs auxin gradients by interfering with auxin efflux. In addition, mass spectrometry was used to show that MDCA triggers auxin biosynthesis, conjugation, and catabolism. A similar shift in auxin homeostasis was found in the c4h mutant ref3-2, indicating that MDCA triggers a cross talk between the phenylpropanoid and auxin biosynthetic pathways independent from the observed auxin efflux inhibition. Altogether, our data provide, to our knowledge, a novel molecular explanation for the phytotoxic properties of MDCA.

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Mdca Affects Plant Growth and Development In A Dosedependentmentioning

confidence: 91%

mentioning

confidence: 99%

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The allelochemical MDCA inhibits lignification and affects auxin homeostasis

et al. 2016

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“…Both chitin and chitosan have demonstrated antiviral, antibacterial, and antifungal properties, and have been explored for many agricultural uses [El Hadrami et al 2010]. Chitosan is an exogenous biotic elicitor that is derived from the fungal cell wall [Montesano et al 2003], it has been studied for their effects on phenylpropanoid metabolic enzymes [Chakraborty et al 2009], and secondary metabolite production [Wiktorowska et al 2010].…”

Section: Introductionmentioning

confidence: 99%

Effects of Foliar of the Application Chitosan and Reduced Irrigation on Essential Oil Yield, Total Phenol Content and Antioxidant Activity of Extracts From Green and Purple Basil

Pirbalouti¹,

Malekpoor²,

Salimi³

et al. 2017

Acta Sci. Pol. Hortorum Cultus

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Phenolic compounds are naturally occurring substances in plants. Basil (Ocimum basilicum L.) belongs to the family Lamiaceae, is a good source of phenolic compounds and natural antioxidants. Elicitors can activate specific genes involved in secondary metabolite biosynthesis. Chitosan, as elicitor, is a natural biopolymer modified from chitin, which is the main structural component of squid pens, cell walls of some fungi and shrimp and crab shells. On the other hand, water deficit stress is one of the most abiotic stress, which effects on the levels of secondary metabolites. To evaluate the effect of chitosan and different irrigation regimes on essential oil yield, total phenol content and antioxidant activity of extracts from green and purple basil (Ocimum basilicum L.), an experiment was conducted at Shahrekord, southwestern Iran. Treatments comprised control, 0.0, 0.2, and 0.4 g·L -1 chitosan applied to plants under normal irrigation, slight and mild drought stress conditions. Results indicated that the different levels of chitosan and irrigation had significant effects on the essential oil yield, total phenol content, and the antioxidant activity of the extracts. Foliar-applied chitosan in particular 0.4 g·L -1 increased total phenolic content in the basil as compared to untreated plants. In conclusion, it is suggested that the foliar application of chitosan as an elicitor could be a promising material used to increase biological activity and pro-health functional value of basil plants.

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Optimizing antidiabetic properties of Galega officinalis extract: Investigating the effects of foliar application of chitosan and salicylic acid

Angouti,

Nourafcan,

Saeedi Sar

et al. 2024

Food Science & Nutrition

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Diabetes poses a significant global health burden, demanding safe and effective therapeutic interventions. Medicinal plants offer promising avenues for natural diabetic management. Galega officinalis (goat's rue) has long been recognized for its hypoglycemic potential, but optimizing its phytochemical content and antidiabetic activity remains a key challenge. This study aimed to address this aspect by investigating the impact of foliar application of chitosan and salicylic acid on the physiological and phytochemical properties of G. officinalis, and subsequently evaluating its antidiabetic efficacy compared to that of the established drug metformin. A randomized complete block design with three replications was employed. Laboratory mice were divided into treatment groups receiving G. officinalis extract from plants sprayed with four salicylic acid concentrations (0.5–3 mM/L) and four chitosan concentrations (0–0.8 g/L). Blood glucose levels and various physiological parameters were assessed. Chitosan at 0.4 g/L and salicylic acid at 2 mM significantly enhanced the growth, photosynthetic pigments, and antioxidant activity of G. officinalis. Notably, the extract from plants treated with 3 mM salicylic acid exhibited the highest total alkaloid content, a potential contributor to antidiabetic activity. In a separate study, diabetic mice treated with this optimized G. officinalis extract (50 mg/kg) exhibited significantly greater blood glucose reductions compared to those treated with metformin (500 mg). This study demonstrates the potential of chitosan and salicylic acid in optimizing the beneficial properties of G. officinalis. The extract derived from plants treated with 3 mM salicylic acid displayed superior blood glucose‐lowering efficacy compared to metformin, suggesting its promising role as a potential natural antidiabetic therapy. Further research is warranted to elucidate the specific bioactive compounds responsible for this enhanced activity and translate these findings into clinical applications.

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Accumulation of phenylpropanoid derivatives in chitosan-induced cell suspension culture of Cocos nucifera

Cited by 59 publications

References 40 publications

The allelochemical MDCA inhibits lignification and affects auxin homeostasis

The allelochemical MDCA inhibits lignification and affects auxin homeostasis

Effects of Foliar of the Application Chitosan and Reduced Irrigation on Essential Oil Yield, Total Phenol Content and Antioxidant Activity of Extracts From Green and Purple Basil

Optimizing antidiabetic properties of Galega officinalis extract: Investigating the effects of foliar application of chitosan and salicylic acid

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