1989
DOI: 10.1099/00221287-135-12-3505
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Accumulation of Polyhydroxy Alcohols by Hansenula anomala in Response to Water Stress

Abstract: The responseoftheyeast Hansenulaanomala toareduction inwater activity (a,)from0.998 to0.925 (adjusted with glucose or NaC1) was monitored. Natural abundance 3C NMR spectroscopy and HPLC analysis revealed that the type of carbon source determined which polyols were present intracellularly at 0.998 a,. At 0.95 a, (NaCl), glycerol was accumulated in all instances irrespective of the type of carbon source indicating the primary role of glycerol in osmoregulation. The carbon source had a bearing only on which other… Show more

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Cited by 25 publications
(24 citation statements)
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“…Similar observations have been made for some halotolerant/ halophilic eubacteria (Galinski & Trüper, 1994), which can change the composition of the solute pool according to growth phase and growth medium. In some halotolerant yeast species, the amount and type of polyol accumulation are also related to the growth phase (Nobre & 1985) and the carbon sources used for growth (Van Eck et al, 1989).…”
Section: Discussionmentioning
confidence: 99%
“…Similar observations have been made for some halotolerant/ halophilic eubacteria (Galinski & Trüper, 1994), which can change the composition of the solute pool according to growth phase and growth medium. In some halotolerant yeast species, the amount and type of polyol accumulation are also related to the growth phase (Nobre & 1985) and the carbon sources used for growth (Van Eck et al, 1989).…”
Section: Discussionmentioning
confidence: 99%
“…Methods for the determination of biomass, cell volume, polyol concentration, calculation of the intracellular a, and the identification of accumulated solutes by 13C-NMR spectroscopy were described previously (Van Eck et al, 1989).…”
Section: Methodsmentioning
confidence: 99%
“…The main solutes accumulated in yeasts exposed to osmotic stress are polyhydroxy alcohols (polyols) such as glycerol, D-arabitol, Dmannitol and meso-erythritol (Spencer & Spencer, 1978), and are compatible with metabolic activity (known as compatible solutes ; . The polyol accumulated is related to the yeast species, the growth phase of the yeast (Nobre & da Costa, 1985) and the carbon source used for growth (Van Eck et al, 1989). The terms ' osmophilic' and ' osrnotolerant ' have been widely used to describe the water relations of yeasts.…”
Section: Introductionmentioning
confidence: 99%
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“…Cells were harvested (3500 × g, 5 min), resuspended in fresh glucose-YNB medium with or without 50 g/l NaCl and grown at 30 • C for 3 h. Cells (1 ml) were harvested by centrifugation and washed twice with fresh media with or without 50 g/l NaCl. Intracellular glycerol was extracted by boiling the washed cell suspension in Tris lysis buffer (van Eck et al 1989) for 10 min, followed by centrifugation (1000 × g, 5 min) to remove the debris. The supernatant was retained for analysis.…”
Section: Determination Of Intracellular Glycerol Concentrationmentioning
confidence: 99%