1975
DOI: 10.1128/jb.121.3.883-891.1975
|View full text |Cite
|
Sign up to set email alerts
|

Accumulation of the capacity of initiation of deoxyribonucleic acid replication in Escherichia coli

Abstract: Several temperature-sensitive initiation mutants of Escherichia coli were examined for the ability to initiate more than one round of replication after being held at nonpermissive temperature for approximately 1.5 generation equivalents. The capacity for initiation was measured by residual synthesis experiments and rate experiments under conditions where protein synthesis and ribonucleic acid synthesis were inhibited. Results of the rate and density transfer experiments suggest that the cells may initiate more… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

3
17
0

Year Published

1982
1982
2003
2003

Publication Types

Select...
8

Relationship

0
8

Authors

Journals

citations
Cited by 32 publications
(20 citation statements)
references
References 21 publications
3
17
0
Order By: Relevance
“…Transcription in seqA and dam mutants of E. coli PC2 dnaC2(ts) aligned for initiation of chromosome replication The effects of seqA and dam mutations on the coupling between chromosome replication and transcription of gidA, mioC and dnaA were determined in dnaC2 (ts) mutants aligned for initiation of chromosome replication by temperature shifts. To align initiation, cultures of PC2, PC2 seqA1, PC2 seqA2 and PC2 dam16 growing exponentially in glucose-Casamino acids minimal medium were shifted from 30ЊC (permissive temperature) to 40ЊC (nonpermissive temperature) for approximately 1.5 mass doublings and then returned to 30ЊC (Schuback et al, 1973;Evans and Eberle, 1975;Hanna and Carl, 1975;Helmstetter and Krajewski, 1982;Bogan and Helmstetter, 1996;Zhou et al, 1997). The cultures of seqA and dam mutants grew slightly slower than PC2 and contained increased numbers of filamentous cells.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Transcription in seqA and dam mutants of E. coli PC2 dnaC2(ts) aligned for initiation of chromosome replication The effects of seqA and dam mutations on the coupling between chromosome replication and transcription of gidA, mioC and dnaA were determined in dnaC2 (ts) mutants aligned for initiation of chromosome replication by temperature shifts. To align initiation, cultures of PC2, PC2 seqA1, PC2 seqA2 and PC2 dam16 growing exponentially in glucose-Casamino acids minimal medium were shifted from 30ЊC (permissive temperature) to 40ЊC (nonpermissive temperature) for approximately 1.5 mass doublings and then returned to 30ЊC (Schuback et al, 1973;Evans and Eberle, 1975;Hanna and Carl, 1975;Helmstetter and Krajewski, 1982;Bogan and Helmstetter, 1996;Zhou et al, 1997). The cultures of seqA and dam mutants grew slightly slower than PC2 and contained increased numbers of filamentous cells.…”
Section: Resultsmentioning
confidence: 99%
“…Indeed, a single large increase in [ 3 H]-thymidine incorporation was seen in the seqA and dam mutants (Fig. 6), rather than the two bursts normally observed in PC2 because of the eclipse between the first two rounds of replication after the shift (Schuback et al, 1973;Evans ᮊ 1997 Blackwell Science Ltd, Molecular Microbiology, 26, 889-896 and Eberle, 1975;Hanna and Carl, 1975;Helmstetter and Krajewski, 1982;Bogan and Helmstetter, 1996;Zhou et al, 1997).…”
Section: Dna Replication During Temperature Shiftsmentioning
confidence: 95%
“…a round of replication initiates coincident with the temperature shift down and then again about 25 to 30 min later. This interval between the first and second initiation events is independent of protein synthesis (Schuback et ai, 1973;Hanna and Carl, 1975;Evans and Eberle, 1975;Helmstetter and Krajewski, 1982). so all proteins required for Initiation are present and the delay must therefore be related to an alternate requirement for Initiation.…”
Section: Discussionmentioning
confidence: 99%
“…In such a scheme, methylation of these two GATC sites would provide a timing mechanism separating consecutive initiation events, and may account for the time lag observed between initiation events (Evans and Eberle, 1975;Helmstetter and Krajewski, 1982); some evidence for this possibility is presented by Messer et al (accompanying paper). These possible functions of GATC methylation in initiation, as well as others, are of course not mutually exclusive, and the state of methylation of different GATC sites may be important, or essential, in more than one step in DNA initiation.…”
Section: Discussionmentioning
confidence: 99%