Accuracy and precision guidelines for optimal breeding time in bitches using in-house progesterone measurement compared with chemiluminescent microparticle immunoassay

Abstract

Background and Aim: The concentration of serum progesterone is commonly used to determine the optimal mating time in bitches, and to diagnose reproductive-related abnormalities. This study aims to compare the serum progesterone results obtained by rapid fluorescence immunochromatography assay (RFICA) with those obtained by chemiluminescent microparticle immunoassay (CMIA) from the same serum samples to develop a standard guideline for optimal breeding time. Materials and Methods: Serum progesterone levels were measured in 124 bitches using RFICA and CMIA. Simple linear regression and correlation analyses were performed to analyze the data. The percentage difference between the maximum and minimum progesterone values in the same serum sample in the same assay was compared using Wilcoxon's rank-sum test. Results: The present study showed a strong linear dependence of the results obtained by RFICA on those obtained by CMIA as R2=0.8976, with regression coefficient of 0.9474 and p<0.05, including the regression model was CMIA = (0.9483 × RFICA) - 0.761. Moreover, five critical measurement times during estrous in bitches showed statistically significant differences (p<0.05), except at the fertilizable period, which showed a non-significant difference (p>0.05). Conclusion: This study demonstrated that it is presumably acceptable to use the RFICA and CMIA methods interchangeably for quality progesterone measurements in serum samples from bitches. However, when considering the use of the RFICA method, it is advisable to carefully interpret the results and follow the interpretation guidelines. Finally, RFICA in the present study provides a reliable and convenient option for veterinarian practitioners to measure canine progesterone levels in-house.

Assessment of Vcheck® analyzer for rapid progesterone concentration measurement including recommendations for achieving the optimal breeding time in bitches

“…However, in 2014, the CLIA method was widely accepted and applied for progesterone measurement [ 25 – 27 ]. CMIA, an advanced adaptation of CLIA, is currently employed by veterinary reference laboratories in Thailand [ 18 ]. It’s important to note that both CLIA and CMIM have drawbacks, as they require several hours to several days for processing, depending on the laboratory’s location.…”

Assessing the precision of point-of-care immunological analyzer for swift progesterone measurement and guidance for determining the optimal breeding time in bitches

“…At present, CLIA is displacing RIA in a multitude of diagnostic laboratories, gaining traction among veterinary practitioners as a preferred method for quantifying progesterone [30,31]. The CMIA represents a sophisticated and advanced iteration of CLIA, with practical implementation in veterinary reference laboratories throughout Thailand [22].…”

“…Within the domain of veterinary practice, diverse techniques exist for quantifying sP4, encompassing radioimmunoassay (RIA) [14,15] liquid chromatography-tandem mass spectrometry (LC-MS) [16][17][18], and chemiluminescence immunoassay (CLIA) [15,[19][20][21]. Furthermore, recent progress encompasses the introduction of point-of-care sP4 measurement analyzers, exemplified by the rapid fluorescence immunochromatography assay (RFICA) and surface plasmon field-enhanced fluorescence spectroscopy (SPFS) [22][23][24][25].…”

Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase

“…In any case, a method for measuring steroids must be validated specifically for the target species and sample matrix. This is especially true for methods without prior sample processing (direct assays), which usually include the commercially available methods for use in dogs [18,19].…”