Objective: To evaluate the social smile symmetry using three-dimensional (3D) stereophotogrammetric images. Materials and Methods: The study was conducted with 3D facial images of 30 individuals (age range 13-25 years). The rest position was considered as the reference image and the social smile image was aligned on this image using the best-fit alignment method. The spatial differences between the same points established on both images using 3D analyses were determined for right and left points in X, Y, and Z planes. Results: The highest difference related to spatial distance in right and left points was À0.56 mm (95% confidence interval [CI], À1.19, 0.06 mm) between right and left commissure (Com) points. The difference was not significant, and the Bland-Altman upper and lower limits were À3.85 mm and 2.71 mm, respectively. The highest difference for the transversal plane was found in Com points, similarly to the spatial distance (mean: 0.50 mm, 95% CI, À2.62, 1.02 mm). The differences between the changes in the left and right points in the Y and Z plane were not significant (P . .05). Conclusions: The social smile was observed to show asymmetry in varying amounts in the different directions. Asymmetry increases in some cases, specifically for the corners of the mouth.