Accuracy of Accusport for measurement of lactate concentrations in equine blood and plasma

Abstract

Summary The aim of this study was to investigate correlations between lactate concentrations in equine whole blood and plasma measured with Accusport1 and Yellow Springs Instruments (YSI2) (2300) methods. The effect of packed cell volume (PCV) on the accuracy of Accusport was also investigated. Blood samples were collected from Thoroughbred horses at 5–10 min intervals after a treadmill exercise test. Blood was added to NaEDTA (for PCV measurement) and to 2 tubes containing lithium heparin anticoagulant (for lactate assays). At concentrations greater than 10 mmol/1, Accusport1 greatly underestimated the blood lactate concentration. Accusport1 also underestimated blood lactate concentration when PCV were greater than 53%. Accusport1 accurately measured plasma lactate concentration in the range 0.8–20 mmol/1.

Assessment of a Point-of-Care Lactate Monitor in Emergency Admissions of Adult Horses to a Referral Hospital

“…23,25 Some of these same monitors have been evaluated in healthy, exercising horses and have been shown to be useful. [20][21][22] However, aside from a relatively small study in horses with colic, 24 the utility of these devices in abnormal horses has not been assessed.…”

“…This result is similar to the findings of some earlier reports examining the accuracy and reliability of the same POC monitor in exercising Thoroughbred horses. [20][21][22] In 1 study, the monitor's accuracy was compared with a well-accepted reference technique that used linear regression analysis. 20 Good association was found between whole blood measurements on the Accutrend and reference technique but only for lactate concentrations ,10 mM and PCV ,53%.…”

“…[20][21][22] In 1 study, the monitor's accuracy was compared with a well-accepted reference technique that used linear regression analysis. 20 Good association was found between whole blood measurements on the Accutrend and reference technique but only for lactate concentrations ,10 mM and PCV ,53%. Similar to the current report, the highest correlation with the reference technique was found when plasma was used in the POC monitor.…”

“…19 The accuracy and reliability of some POC monitors have been evaluated in clinically normal exercising humans and horses, human critical care patients, and a small number of horses with colic. [19][20][21][22][23][24] However, their utility has not yet been established for clinically abnormal horses, where performance of these monitors might be altered by the clinical disease of the patient. To be clinically useful, the accuracy of a POC monitor should be excellent within the reference range and remain acceptable over the clinically relevant range in the patient population of concern.…”

Simple Lactate Measurement in Horses Using a Portable Lactate Analyzer with Lancet Skin Punctures under Field Conditions

“…In horses, blood lactate has been measured during treadmill tests [14,18] and running tests under field conditions [5,9]. It has been reported that the Accusport (AC) portable analyzer should be used only to measure lactate concentration in equine whole blood when Ht is less than 53% and lactate concentration is less than 10 mmol/l [4].…”