2021
DOI: 10.1371/journal.pntd.0009648
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Accuracy of an experimental whole-blood test for detecting reactivation of echinococcal cysts

Abstract: Background Cystic echinococcosis (CE) is a complex disease for which clear understanding of clinical manifestations is needed to avoid misdiagnosis, inappropriate treatment, and severe complications. We evaluated the accuracy of a whole-blood stimulation test based on Interleukin (IL)-4 detection in response to Antigen B (AgB) of Echinococcus granulosus sensu lato to discriminate cyst viability and detect cyst reactivation in patients with hepatic CE. Methodology/Principal findings Thirty patients with CE3b … Show more

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Cited by 7 publications
(1 citation statement)
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“…Washing steps were done with 300 μl/well of PBS containing 0.05% Tween20. Native Antigen B (AgB) [34][35][36][37][38] and an AgB peptide pool [36] were used as experimental positive controls. The OD read for the wells where no antigen was adsorbed ("peptide-/serum+/secondary antibody+") was considered as the background.…”
Section: Elisamentioning
confidence: 99%
“…Washing steps were done with 300 μl/well of PBS containing 0.05% Tween20. Native Antigen B (AgB) [34][35][36][37][38] and an AgB peptide pool [36] were used as experimental positive controls. The OD read for the wells where no antigen was adsorbed ("peptide-/serum+/secondary antibody+") was considered as the background.…”
Section: Elisamentioning
confidence: 99%