Accuracy of assessment of luteal morphology and luteal blood flow for prediction of early pregnancy in goats

Cosentino, Isabel Oliveira; Balaro, M. F. A.; Leal, Felipe Seabra Cardoso; Carvalho, Ana Beatriz da Silva; Souza, Paula Renata Cortat de; Arashiro, E. K. N.; Brandão, Felipe Zandonadi

doi:10.1016/j.theriogenology.2018.08.007

Cited by 19 publications

(13 citation statements)

References 24 publications

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“…Also in non‐pregnant alpacas, luteal vascularization increased after induction of ovulation, reaching its maximum values between Days 7 and 9 after GnRH analog (Gonadorelin acetate) administration and then decreased in coincidence with plasma P4 concentration (Norambuena et al, ). In different species, it has been reported that both luteal blood flow and plasma P4 concentration simultaneously decreased in non‐pregnant animals while they remained elevated or continued increasing during early pregnancy (mares: Ginther et al, ; Šichtař et al, ; cows: Herzog et al, ; Hassan et al, ; ewes: Figueira et al, ; Arashiro et al, ; goats: Balaro et al, ; Cosentino et al, ; buffaloes: Di Francesco et al, ; Vecchio et al, ). In fact, luteolysis onset is associated with a decrease in luteal vascularization and plasma P4, while pregnancy establishment and maintenance require a well vascularized and functional CL (Cosentino et al, ; Hassan et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…In pregnant cows, it has been reported that both luteal blood flow area and P4 increased between Days 15 and 18 post oestrus, in coincidence with the MRP period, while in non‐pregnant animals both variables decreased (Hassan et al, ; Herzog et al, ). In contrast, in ewes and goats differences in CL vascularization between pregnant and non‐pregnant animals have been observed after the occurrence of MRP (Arashiro et al, ; Cosentino et al, ). These are in accordance with the results observed in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…Vascular development is essential for CL formation and maintenance so the impairment of this process may lead to luteal insufficiency and affect pregnancy establishment (Chouhan et al, ). In fact, in different species CL vascularization is associated with plasma P4 concentration and so it has been proposed that luteal vascularization could be a good predictor of pregnancy rates (cattle: Acosta, Hayashi, Ohtani, & Miyamoto, ; Mann, ; Pinaffi et al, ; Kanazawa et al, ; sheep: Figueira et al, ; Arashiro et al, ; goat: Balaro, Santos, Moura, Fonseca, & Brandão, ; Cosentino et al, ; horse: Ginther, Gastal, Gastal, Utt, & Beg, ; Ishak, Bashir, Gastal, & Gastal, ). Similarly, in non‐pregnant llamas, plasma P4 and CL vascularization showed a similar profile after pharmacological induction of ovulation; suggesting an association between both variables (Ulloa‐Leal, Bogle, Adams, & Ratto, ).…”

Section: Introductionmentioning

confidence: 99%

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Corpus luteum vascularization during the maternal recognition of pregnancy in llamas (Lama glama)

Gallelli

Bianchi

Zampini

et al. 2019

Reprod Domestic Animals

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The aim of this study was to characterize corpus luteum vascularization and its association with plasma progesterone concentration in early stages of pregnancy, when maternal recognition of pregnancy is expected to occur. In all animals, both plasma progesterone concentration and corpus luteum vascularization increased from Day 6 to Day 8 post‐mating and afterwards in non‐pregnant llamas they started to decrease to reach basal levels around Days 12 to 14 post‐mating, while in pregnant animals, both variables remained elevated until the end of the study. A lineal positive relationship between corpus luteum vascularization and plasma progesterone concentration was observed in pregnant (r2 = .46, p < .0001) and non‐pregnant llamas (r2 = .66, p < .0001). Pregnant animals showed higher plasma progesterone concentration and corpus luteum vascularization than the non‐pregnant ones from Day 12 post‐mating until the end of the study (p ˂ .05 and p ˂ .01, respectively). These results suggest that maternal recognition of pregnancy should occur before Day 12 post‐mating in order to expand luteal lifespan, maintaining corpus luteum vascularization and progesterone production. Also, the assessment of CL vascularization area could be a useful and non‐invasive method for early pregnancy diagnosis due to its association with plasma progesterone concentration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Corpus luteum vascularization during the maternal recognition of pregnancy in llamas (Lama glama)

Gallelli

Bianchi

Zampini

et al. 2019

Reprod Domestic Animals

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“…US scans were performed using a portable device (Sonoscape S6) with a 7.5 MHz linear rectal transducer adapted for use in small ruminants, with does in a standing position. All does were re‐examined on Days 16, 21 and 24 for assessment of luteal morphology (score 1: near anechoic, heterogeneous and coarse granulation; 2: hypoechoic, homogeneous and fine granulation; and 3: echogenic, homogeneous and fine granulation) and blood flow (score 1: 0% to 25% vascularization; 2: 25% to 50%; 3: 50% to 75%; and 4: 75% to 100%), with scores above 2 being related to pregnancy (Cosentino et al., 2018). The first evaluation (Day 16) aimed to count the viable CLs before the second P4 device; the second evaluation (Day 21) was for early pregnancy diagnosis at time of sponge removal; and the third evaluation (Day 24) was to evaluate possible early pregnancy losses and further effects of the second P4 device.…”

Section: Methodsmentioning

confidence: 99%

Early resynchronization protocols for goats: Progestogens can be used prior to an early pregnancy diagnosis without affecting corpus luteum functionality

Cosentino

Balaro

Leal

et al. 2020

Reprod Domestic Animals

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Resynchronization protocols have been studied and successfully performed in cattle since the beginning of the century (Bartolome et al., 2005; Sani et al., 2011; Stevenson et al., 2003). With variations in timing and procedures, such protocols are primarily used to reduce the calving interval by inducing more synchronic ovulations during the breeding season. Hence, early resynchronization protocols are being studied to allow fixed time artificial insemination (FTAI) at intervals similar to the natural oestrous cycle (Palhão et al., 2020; Pugliesi et al., 2019), for which a new progesterone (P4) device should be inserted before the pregnancy diagnosis. The corpus luteum (CL) is a temporary endocrine gland of high importance in the maintenance of pregnancy, especially for goats, since ewes and cows can sustain late pregnancy without it (Casida

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“…A n early and accurate pregnancy diagnosis is essential to improve efficiency and the reproductive management of ruminants (Pieterse et al, 1990;Cosentino et al, 2018). A successful detection of pregnancy at earliest period leads shortens calving intervals and subsequently increases production, causing huge economic benefits (Dillon et al, 2006;Diskin et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Early Detection of Pregnancy in Goats Based on Protein Expression Pattern using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)

Inyawilert¹,

Piarked²,

Joemplang³

et al. 2019

Adv. Anim. Vet. Sci.

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Several methods for early pregnancy diagnosis in ruminants have been developed. Some of them are applicable in the field, but not enough accurate. Hence, the aim of this study was to investigate pregnancy detection in goats at earlier stages, that is, 7, 14, 21, and 28 days post-artificial insemination (post-AI). Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE gel electrophoresis) was used to determine the pattern of protein expression. Moreover, ultrasound was used to confirm pregnancy 45 days post-AI. Nine of female goats were under ovsynch protocol and mating was initiated. The results showed that the pattern of protein expression was different between pregnant and non-pregnant goats with molecular weights of 42 kDa and 52 kDa. The protein fraction at 42 kDa was higher on day 21 and 28 post AI. In addition, the 52 kDa protein fractions was higher at day 7, 14, 21, and 28 post-AI in pregnant than in the non-pregnant goats. Ultrasonography was used 45 days after AI to confirm the results of SDS-PAGE gel electrophoresis. The ultrasound results were consistent with SDS-PAGE gel electrophoresis. Therefore, serum protein fractions of 42 kDa and 52 kDa might serve as protein biomarkers for early detection of pregnancy in goats.

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Accuracy of assessment of luteal morphology and luteal blood flow for prediction of early pregnancy in goats

Cited by 19 publications

References 24 publications

Corpus luteum vascularization during the maternal recognition of pregnancy in llamas (Lama glama)

Corpus luteum vascularization during the maternal recognition of pregnancy in llamas (Lama glama)

Early resynchronization protocols for goats: Progestogens can be used prior to an early pregnancy diagnosis without affecting corpus luteum functionality

Early Detection of Pregnancy in Goats Based on Protein Expression Pattern using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)

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