Accuracy of clustered regularly interspaced short palindromic repeats (CRISPR) to diagnose COVID-19, a meta-analysis

Wang, Song; Hu, Jiayi; Sui, Chuanying; He, Guangliang; Qu, Zhigang; Chen, Xiaofei; Wang, Yashan; Guo, Dingjie; Liu, Xin

doi:10.1016/j.micpath.2022.105498

Cited by 5 publications

(4 citation statements)

References 68 publications

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“…Cas13 directly acts on the genetic material of coronaviruses, that is RNA, so the detection step is relatively simple and the detection time is shorter compared with Cas12. Both Cas12/Cas13 combined with RPA/RAA techniques show extremely high sensitivity and specificity in diagnosing COVID-19, and the same conclusion was made in Wang's study [45]. In sample type analysis, to unify the criteria, we did not include the literature on multiple types of samples containing nasopharyngeal swabs in the analysis.…”

Section: Plos Onementioning

confidence: 92%

Diagnostic efficiency of RPA/RAA integrated CRISPR-Cas technique for COVID-19: A systematic review and meta-analysis

Zhang

Xia²,

Shen³

et al. 2022

PLoS ONE

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Objective To evaluate the diagnostic value of recombinase polymerase/ aided amplification (RPA/RAA) integrated clustered regularly interspaced short palindromic repeats (CRISPR) in the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Methods We searched relevant literature on CRISPR technology for COVID-19 diagnosis using "novel coronavirus", "clustered regularly interspaced short palindromic repeats" and "RPA/RAA" as subject terms in PubMed, Cochrane, Web of Science, and Embase databases. Further, we performed a meta-analysis after screening the literature, quality assessment, and data extraction. Results The pooled sensitivity, specificity and a rea under the summary receiver operator characteristic curve (AUC) were 0.98 [95% confidence interval (CI):0.97–0.99], 0.99 (95% CI: 0.97–1.00) and 1.00 (95% CI: 0.98–1.00), respectively. For CRISPR-associated (Cas) proteins-12, the sensitivity, specificity was 0.98 (95% CI: 0.96–1.00), 1.00 (95% CI: 0.99–1.00), respectively. For Cas13, the sensitivity and specificity were 0.99 (95% CI: 0.97–1.00) and 0.95 (95% CI: 0.91–1.00). The positive likelihood ratio (PLR) was 183.2 (95% CI: 28.8, 1166.8); the negative likelihood ratio (NLR) was 0.02 (95% CI: 0.01, 0.03). Conclusion RPA/RAA integrated with CRISPR technology is used to diagnose coronavirus disease-19 (COVID-19) with high accuracy and can be used for large-scale population screening.

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Section: Plos Onementioning

confidence: 92%

Diagnostic efficiency of RPA/RAA integrated CRISPR-Cas technique for COVID-19: A systematic review and meta-analysis

Zhang

Xia²,

Shen³

et al. 2022

PLoS ONE

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“…New detection tools worked on the clustered regularly interspaced short palindromic repeats/Cas (CRISPR-Cas) system, which with its better detection accurate measures, has come up for better diagnosis [ 1 , 14 ]. Genomic research, genome editing, gene therapy and genome mapping have all been transformed by the RNA-guided endonuclease (RGEN)-based CRISPR/Cas system [ 15 ]. Adaptive immunity systems opposed to bacteria and archaea are the CRISPR and Cas proteins.…”

Section: Reviewmentioning

confidence: 99%

Role of Technology in Detection of COVID-19

Lohiya¹,

Pathak²

2022

Cureus

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The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus caused coronavirus infection termed as COVID-19, an illness that has spread devastation all over the world. It was developed first in China and had swiftly spread throughout the world. COVID has created imposed burden on health in the lives of all individuals around the globe. This article provides a number of unprecedented detection technologies used in the detection of infection. COVID has created a large number of symptoms in the young, adolescent as well as elderly population. Old age people are susceptible to fatal serious symptoms because of low immunity. With these goals in mind, this article includes substantial condemning descriptions of the majority of initiatives in order to create diagnostic tools for easy diagnosis. It also provides the reader with a multidisciplinary viewpoint on how traditional approaches such as serology and reverse transcriptase polymerase chain reaction (RT-PCR) along with the frontline techniques such as clustered regularly interspaced short palindromic repeats (CRISPR)/Cas and artificial intelligence/machine learning have been utilized to gather information. The story will inspire creative new ways for successful detection therapy and to prevent this pandemic among a wide audience of operating and aspiring biomedical scientists and engineers.

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“…However, challenges persist in CRISPR-based gene therapy, with one key hurdle being the achievement of efficient delivery to targeted cells [11,12]. While both viral vectors, like adeno-associated viral vectors [13,14], and non-viral vectors, such as lipid nanoparticle [15-17] and cationic polymers [18,19], have been utilized for CRISPR delivery, the effectiveness of these delivery systems continues to face challenges due to the pre-existing immunity risks, cargo size limitations, and safety concerns [20].Plasmid DNA (pDNA) is an exceptional vector for CRISPR-based gene therapy due to its inherent stability, ease of large-scale preparation, and cost-effectiveness [21]. However, CRISPR pDNA must overcome various physiologic barriers to exert its functions, including cell…”

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confidence: 99%

mentioning

confidence: 99%

Short cell-penetration peptide conjugated bioreducible polymer enhances gene editing of CRISPR system

Wang,

Cai,

et al. 2024

J Nanobiotechnol

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CRISPR-based gene therapy offers precise targeting and specific editing of disease-related gene sequences, potentially yielding long-lasting treatment effects. However, efficient delivery remains a significant challenge for its widespread application. In this study, we design a novel short peptide-conjugated bioreducible polymer named TSPscp as a safe and effective delivery vector for the CRISPR system. Our results show that TSPscp markedly boosts transcriptional activation and genome editing activities of multiple CRISPR systems as confirmed by decomposition-seq and Deep-seq, which is resulted from its capability in facilitating delivery of plasmid DNA by promoting cellular uptake and lysosomal escape. Additionally, TSPscp further enhances genome editing of CRISPR by delivery of minicircle DNA, a condensed form of regular plasmid DNA. More importantly, TSPscp significantly improves delivery and genome editing of CRISPR system in vivo. In summary, our study highlights TSPscp as a promising delivery tool for CRISPR applications in vivo. Graphical Abstract

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Accuracy of clustered regularly interspaced short palindromic repeats (CRISPR) to diagnose COVID-19, a meta-analysis

Cited by 5 publications

References 68 publications

Diagnostic efficiency of RPA/RAA integrated CRISPR-Cas technique for COVID-19: A systematic review and meta-analysis

Diagnostic efficiency of RPA/RAA integrated CRISPR-Cas technique for COVID-19: A systematic review and meta-analysis

Role of Technology in Detection of COVID-19

Short cell-penetration peptide conjugated bioreducible polymer enhances gene editing of CRISPR system

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