Accuracy of Platelet Counting by Optical and Impedance Methods in Patients with Thrombocytopaenia and Microcytosis

Boulassel, Mohamed Rachid; Al-Farsi, Raya; Al-Hashmi, Sulaiman; Al-Riyami, Hamad; Khan, Hammad; Kindi, Salam Al

doi:10.18295/squmj.2015.15.04.004

Cited by 11 publications

(12 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The superiority between the optical and the impedance methods used in the automatic blood analyzers has been a frequently debated topic [19]. Furthermore, a new fluorescence method for platelet detection has aroused the attention of scholars [20].…”

Section: Discussionmentioning

confidence: 99%

Compare the accuracy and precision of Coulter LH780, Mindray BC-6000 Plus, and Sysmex XN-9000 with the international reference flow cytometric method in platelet counting

Sun

Huang

et al. 2019

PLoS ONE

View full text Add to dashboard Cite

Objective The aim of this study is to evaluate the performance of different platelet counting methods (optical, impedance, fluorescence and hand counting) applied in different analysers by comparing with the international flow cytometric reference method (IRM). Methods A total of 333 blood samples from different subgroups (168 cases with thrombocytopenia, 136 cases with normal platelet counts and 29 cases with thrombocytosis) were tested. Regarding IRM as the gold standard, we compared the accuracy and precision of different platelet count methods; i.e. LH780 (impedance), BC-6000 Plus (optical (O) and impedance (I)), Sysmex XN-9000 (optical (O), impedance (I), fluorescence (F)), and hand counting. Results Sysmex XN-9000-F (r = 0.988) had the best correlation with IRM for thrombocytopenic samples; BC-6000 Plus-I (r = 0.966) was more relevant to IRM than any other method for samples with normal platelet counts. Correlation between Sysmex XN-9000-I (r = 0.960) and IRM was the highest among these methods for samples with thrombocytosis. For bias evaluation, the average bias of Sysmex XN-9000-F was -1.5 × 10 9 /L (95% LA = -9.4 to +6.4) for samples with thrombocytopenia, compared with IRM. BC-6000 Plus-I had a small mean difference with IRM for samples with normal platelet counts or thrombocytosis. Moreover, all evaluated methods had acceptable sensitivity, specificity, and concordance rates as compared with IRM in the diagnosis of thrombocytopenia and thrombocytosis. Conclusions Platelet counting by Sysmex XN-9000-F is more accurate than other methods for thrombocytopenic samples. BC-6000 Plus-I has superior association and consistency for normal platelet counts. As for thrombocytosis patients, Sysmex XN-9000-I has the highest correlation with IRM while Sysmex XN-9000-O has the highest diagnosis efficacy.

show abstract

Section: Discussionmentioning

confidence: 99%

Compare the accuracy and precision of Coulter LH780, Mindray BC-6000 Plus, and Sysmex XN-9000 with the international reference flow cytometric method in platelet counting

Sun

Huang

et al. 2019

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…The authors compared PLT-O and PLT-F in the study group and found that the correlation between methods was excellent (data not shown). Previous studies [18][19][20] have described good reproducibility and correlation between optical fluorescent platelet counts and IRM, although the populations in these studies did not include patients with thalassemia. Our data confirmed that PLT-O and PLT-F also correlated well with IRM in patients with thalassemia.…”

Section: Discussionmentioning

confidence: 99%

Performance Evaluation of Automated Impedance and Optical Fluorescence Platelet Counts Compared With International Reference Method in Patients With Thalassemia

Tantanate¹,

Khowawisetsut²,

Pattanapanyasat

2017

Archives of Pathology &Amp; Laboratory Medicine

View full text Add to dashboard Cite

show abstract

“…On the other hand, platelet clumps and giant platelets owing to their large size are not counted as platelets in automated analyzers. All these lead to inaccurate platelet counts in an automated hematology analyzer [8][9][10][11] . Hence platelet count values obtained from automated analyzers should be crosschecked by some other method particularly in cases of thrombocytopenia [12] .…”

Section: Discussionmentioning

confidence: 99%

A novel method to estimate platelet counts from peripheral smears: A study comparing a new method of platelet estimation with existing methods.

Sudalaimuthu¹,

Ganapathy²,

Rajendran³

et al. 2017

APALM

View full text Add to dashboard Cite

Background: Automated hematology analyzers have a low degree of accuracy in predicting platelet counts in patients with thrombocytopenia. Hence automated platelet counts should be crosschecked by reviewing the peripheral blood smear in cases of thrombocytopenia. However existing methods for platelet count estimation from peripheral blood smears have certain drawbacks. Hence we suggest a new method to estimate platelet count from peripheral smear and compared it with the existing methods. Conclusion:Platelet count estimation based on platelet/RBC ratio in ten oil immersion fields and total RBC count is a simple and reliable method to estimate platelet counts from peripheral smears.

show abstract

Accuracy of Platelet Counting by Optical and Impedance Methods in Patients with Thrombocytopaenia and Microcytosis

Abstract: The impedance method significantly overestimated platelet counts in microcytic and thrombocytopaenic blood samples. Further attention is therefore needed to improve the accuracy of platelet counts, particularly for patients with conditions associated with microcytosis.

Cited by 11 publications

References 11 publications

Compare the accuracy and precision of Coulter LH780, Mindray BC-6000 Plus, and Sysmex XN-9000 with the international reference flow cytometric method in platelet counting

Compare the accuracy and precision of Coulter LH780, Mindray BC-6000 Plus, and Sysmex XN-9000 with the international reference flow cytometric method in platelet counting

Performance Evaluation of Automated Impedance and Optical Fluorescence Platelet Counts Compared With International Reference Method in Patients With Thalassemia

A novel method to estimate platelet counts from peripheral smears: A study comparing a new method of platelet estimation with existing methods.

Contact Info

Product

Resources

About