“…Colour changes are more pronounced when the raw material is of poor quality -for example, green discolouration development in canned tuna is attributed to a number of factors, some pertaining to quality status, trimethylamine oxide (TMAO), myoglobin and cysteine concentration and the cooking operation itself (Khayat, 1978). Determination of the combined TMAO and trimethylamine (TMA) content of the raw fish can be used to indicate the probability of greening occurring in the can during the heat process (Yamagata et al ., 1971).…”