Accuracy of Predicting Stages of Bovine Estrous Cycle by Gross Appearance of the Corpus Luteum

Ireland, James J.; Murphee, R.L.; Coulson, Patricia B.

doi:10.3168/jds.s0022-0302(80)82901-8

Cited by 585 publications

(351 citation statements)

References 13 publications

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“…Bovine corpora lutea, 5-10 days post-ovulation [16], were collected fresh from an abattoir. Sheep corpora lutea, 3 days post-ovulation (estimated by use of a vasectomised ram) were obtained from animals slaughtered on the premises by a captivebolt.…”

Section: Tissuementioning

confidence: 99%

Biosynthesis of oxytocin in the corpus luteum

et al. 1984

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In this report we demonstrate that ovine and bovine luteal cells synthesise oxytocin by way of a precursor protein similar to that found in the hypothalamus. Isolated ovine or bovine luteal cells were incubated for up to 12 h with [TS]cysteine. Neurophysin-Sepharose column separation and HPLC of cell extracts demonstrated the presence of [%]oxytocin. Incorporation of [%]cysteine was confirmed by performic acid oxidation. Immunoprecipitation of cell extract with anti-rat oxytocin-neurophysin followed by SDS-PAGE yielded 2 radioactive bands of 14 kDa and 1 l-12 kDa. Immunoprecipitation with anti-oxytocin yielded 1 band at 14 kDa. On SDS-PAGE the 14 kDa band had a similar mobility to rat-hypothalamic oxytocin precursor.

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Section: Tissuementioning

confidence: 99%

Biosynthesis of oxytocin in the corpus luteum

et al. 1984

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“…After rinsing with 70% ethanol, ovarian pairs containing a visible corpus luteum (Ireland et al 1980) were selected for dissection. Individual follicles measuring 3-17 mm in diameter were carefully dissected out and their diameter determined in two different planes using a Vernier caliper.…”

Section: Collection and Processing Of Bovine Tissuesmentioning

confidence: 99%

Characterization of microRNAs differentially expressed during bovine follicle development

Sontakke¹,

Mohammed²,

McNeilly³

et al. 2014

Reproduction

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Several different miRNAs have been proposed to regulate ovarian follicle function; however, very limited information exists on the spatiotemporal patterns of miRNA expression during follicle development. The objective of this study was to identify, using microarray, miRNA profiles associated with growth and regression of dominant-size follicles in the bovine monovular ovary and to characterize their spatiotemporal distribution during development. The follicles were collected from abattoir ovaries and classified as small (4-8 mm) or large (12-17 mm); the latter were further classified as healthy or atretic based on estradiol and CYP19A1 levels. Six pools of small follicles and individual large healthy (nZ6) and large atretic (nZ5) follicles were analyzed using Exiqon's miRCURY LNA microRNA Array 6th gen, followed by qPCR validation. A total of 17 and 57 sequences were differentially expressed (greater than or equal to twofold; P!0.05) between large healthy and each of small and large atretic follicles respectively. Bovine miRNAs confirmed to be upregulated in large healthy follicles relative to small follicles (bta-miR-144, bta-miR-202, bta-miR-451, bta-miR-652, and bta-miR-873) were further characterized. Three of these miRNAs (bta-miR-144, bta-miR-202, and bta-miR-873) were also downregulated in large atretic follicles relative to large healthy follicles. Within the follicle, these miRNAs were predominantly expressed in mural granulosa cells. Further, body-wide screening revealed that bta-miR-202, but not other miRNAs, was expressed exclusively in the gonads. Finally, a total of 1359 predicted targets of the five miRNAs enriched in large healthy follicles were identified, which mapped to signaling pathways involved in follicular cell proliferation, steroidogenesis, prevention of premature luteinization, and oocyte maturation.

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“…In all experiments, only early CL (day 1-4) were used and these were selected based on previously outlined criteria (Ireland et al 1980). The CLs were removed connective tissue trimmed away and then sliced up using scissors.…”

Section: Tissue Collection and Luteal Angiogenesis Culture Systemmentioning

confidence: 99%

FGF2 is crucial for the development of bovine luteal endothelial networks in vitro

Woad¹,

Hammond²,

Mann³

et al. 2009

Reproduction

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The development of the corpus luteum requires angiogenesis, and involves the complex interplay between factors such as vascular endothelial growth factor A (VEGFA), fibroblast growth factor 2 (FGF2) and platelet-derived growth factor (PDGF). However, the relative role of these factors remains to be elucidated. This study used a new physiologically relevant mixed luteal cell culture system to test the hypotheses that: a) FGF2 and VEGFA are critical for bovine luteal angiogenesis; and b) local luteal PDGF signalling stimulates the formation of endothelial networks. Cells were treated with receptor tyrosine kinase inhibitors against VEGFA (SU1498), FGF2 (SU5402) or PDGF (AG1295) activity. After 9 days in culture, endothelial cells were immunostained for von Willebrand factor (VWF) and quantified by image analysis. Highly organised intricate endothelial networks were formed in the presence of exogenous VEGFA and FGF2. The inhibition of FGF2 activity reduced the total area of VWF staining versus controls (O95%; P!0.001). Inhibition of VEGF and PDGF activity reduced the endothelial network formation by more than 60 and 75% respectively (P!0.05). Progesterone production increased in all treatments from day 1 to 7 (P!0.001), and was unaffected by FGF2 or PDGF receptor kinase inhibition (PO0.05), but was reduced by the VEGF receptor inhibitor on days 5 and 7 (P!0.001). In conclusion, this study confirmed that VEGF signalling regulates both bovine luteal angiogenesis and progesterone production. However, FGF2 was crucial for luteal endothelial network formation. Also, for the first time, this study showed that local luteal PDGF activity regulates bovine luteal endothelial network formation in vitro.

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Accuracy of Predicting Stages of Bovine Estrous Cycle by Gross Appearance of the Corpus Luteum

Cited by 585 publications

References 13 publications

Biosynthesis of oxytocin in the corpus luteum

Biosynthesis of oxytocin in the corpus luteum

Characterization of microRNAs differentially expressed during bovine follicle development

FGF2 is crucial for the development of bovine luteal endothelial networks in vitro

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