Accuracy of Real-Time PCR to Study Mycosphaerella graminicola Epidemic in Wheat: From Spore Arrival to Fungicide Efficiency

Selim, Sameh; Roisin-Fichter, Céline; Andry, Jean-Baptiste; Bogdanow, Boris

doi:10.5772/27232

Cited by 4 publications

(5 citation statements)

References 31 publications

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“…We think there is room for improvement in the simulation of epidemic slowdowns during dry periods (Shaw et al, 1990(Shaw et al, , 1991, and also by taking into account the role of airborne ascospores. In addition to the well-established role of pycnidiospores, ascospores may contribute to epidemic development in particular environmental conditions (Hunter et al, 1999;Sameh et al, 2011;Suffert et al, 2011;Duvivier et al, 2013;McDonald and Mundt, 2016;Morais et al, 2016;Suffert et al, 2016). For instance, 'escaped upper leaves' could receive ascospores by wind from other crops, which then allowed a second round of conidial multiplication.…”

Section: Model Evaluation and Complexitymentioning

confidence: 99%

Plant architecture and foliar senescence impact the race between wheat growth and Zymoseptoria tritici epidemics

Robert

Garin²,

Abichou

et al. 2018

Annals of Botany

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We introduce the notion of a 'race' for the colonization of emerging healthy host tissue between the growing canopy and the developing epidemics. This race is 2-fold: (1) an upward race at the canopy scale where STB must catch the newly emerging leaves before they grow away from the spore sources; and (2) a local race at the leaf scale where STB must use the resources of its host before it is caught by leaf apical senescence. The results shed new light on the importance of dynamic interactions between host and pathogen.

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Section: Model Evaluation and Complexitymentioning

confidence: 99%

Plant architecture and foliar senescence impact the race between wheat growth and Zymoseptoria tritici epidemics

Robert

Garin²,

Abichou

et al. 2018

Annals of Botany

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“…The infectious process of the hemibiotrophic fungus Zymoseptoria tritici, responsible for STB, is characterized by an approximately 2 to 3 weeks-long symptomless period. Later on, the fungus switches to a necrotrophic phase, associated with an increase of fungal biomass, the formation of pycnidia in colonized substomatal cavities, necrosis (Kema et al, 1996;Selim et al, 2011Selim et al, , 2014Siah et al 2010a;Ors et al 2017) and the production of fungal cell-wall degrading enzymes (CWDE) (Douaiher et al, 2007a(Douaiher et al, , 2007bSiah et al, 2010a).…”

Section: Introductionmentioning

confidence: 99%

A Plant Nutrient- and Microbial Protein-Based Resistance Inducer Elicits Wheat Cultivar-Dependent Resistance Against Zymoseptoria tritici

et al. 2019

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The induction of plant defense mechanisms by resistance inducers is an attractive and innovative alternative to reduce the use of fungicides on wheat against Zymoseptoria tritici, the responsible agent of Septoria tritici blotch (STB). Under controlled conditions, we investigated the resistance induction in three wheat cultivars with different susceptible levels to STB as a response to a treatment with a sulfur, manganese sulfate, and protein-based resistance inducer (NECTAR Céréales). While no direct antigermination effect of the product was observed in planta, more than 50% reduction of both symptoms and sporulation were recorded on the three tested cultivars. However, an impact of the wheat genotype on resistance induction was highlighted, which affects host penetration, cell colonization, and the production of cell-wall degrading enzymes by the fungus. Moreover, in the most susceptible cultivar Alixan, the product upregulated POX2, PAL, PR1, and GLUC gene expression in both noninoculated and inoculated plants and CHIT2 in noninoculated plants only. In contrast, defense responses induced in Altigo, the most resistant cultivar, seem to be more specifically mediated by the phenylpropanoid pathway in noninoculated as well as inoculated plants, since PAL and CHS were most specifically upregulated in this cultivar. In Premio, the moderate resistant cultivar, NECTAR Céréales elicits mainly the octadecanoid pathway, via LOX and AOS induction in noninoculated plants. We concluded that this complex resistance-inducing product protects wheat against Z. tritici by stimulating the cultivar-dependent plant defense mechanisms.

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“…The qPCR conditions were set as follows: 10 min at 95 • C, followed by 40 cycles of 15 s at 95 • C, and 1 min at 60 • C. All qPCR experiments were performed using the StepOnePlus Real-Time PCR System (Applied Biosystems, Waltham, MA, USA). To calibrate the qPCR analysis, we used serial dilutions of the cloned target sequence of the β-tubulin gene of M. graminicola, ranging from 10 2 to 10 7 copies, as previously described [3]. The results were expressed as the β-tubulin copy number per 100 ng of leaf DNA (BCN 100ng ).…”

Section: Wheat Resistance Induction Against M Graminicola and Wheat B...mentioning

confidence: 99%

“…PGPR-mediated ISR is akin to pathogen-induced systemic acquired resistance (SAR), bolstering the plant's immune system against abiotic and biotic stresses. For instance, Septoria tritici leaf blotch (STB) disease in wheat, caused by Mycosphaerella graminicola, can lead to crop losses exceeding 40% [2,3]. ISR promotes the formation of physical barriers such as callose and lignin, the synthesis of protective compounds in plants, including reactive oxygen species, phytoalexins, and phenolic compounds [4,5].…”

Section: Introductionmentioning

confidence: 99%

PGPR-Soil Microbial Communities’ Interactions and Their Influence on Wheat Growth Promotion and Resistance Induction against Mycosphaerella graminicola

Samain,

Duclercq,

Ait Barka

et al. 2023

Biology

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The efficiency of plant-growth-promoting rhizobacteria (PGPR) may not be consistently maintained under field conditions due to the influence of soil microbial communities. The present study aims to investigate their impact on three PGPR-based biofertilizers in wheat. We used the PGPR Paenibacillus sp. strain B2 (PB2), PB2 in co-inoculation with Arthrobacter agilis 4042 (Mix 2), or with Arthrobacter sp. SSM-004 and Microbacterium sp. SSM-001 (Mix 3). Inoculation of PB2, Mix 2, and Mix 3 into non-sterile field soil had a positive effect on root and aboveground dry biomass, depending on the wheat cultivar. The efficiency of the PGPR was further confirmed by the protection they provided against Mycosphaerella graminicola, the causal agent of Septoria leaf blotch disease. PB2 exhibited protection of ≥37.8%, while Mix 2 showed ≥47.9% protection in the four cultivars tested. These results suggest that the interactions between PGPR and native soil microbial communities are crucial for promoting wheat growth and protection. Additionally, high-throughput sequencing of microbial communities conducted 7 days after PGPR inoculations revealed no negative effects of PB2, Mix 2, and Mix 3 on the soil microbial community structure. Interestingly, the presence of Arthrobacter spp. appeared to mitigate the potential negative effect of PB2 on bacterial community and foster root colonization by other beneficial bacterial strains.

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Accuracy of Real-Time PCR to Study Mycosphaerella graminicola Epidemic in Wheat: From Spore Arrival to Fungicide Efficiency

Cited by 4 publications

References 31 publications

Plant architecture and foliar senescence impact the race between wheat growth and Zymoseptoria tritici epidemics

Plant architecture and foliar senescence impact the race between wheat growth and Zymoseptoria tritici epidemics

A Plant Nutrient- and Microbial Protein-Based Resistance Inducer Elicits Wheat Cultivar-Dependent Resistance Against Zymoseptoria tritici

PGPR-Soil Microbial Communities’ Interactions and Their Influence on Wheat Growth Promotion and Resistance Induction against Mycosphaerella graminicola

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