Accuracy of the Ultra-Rapid Urease Test for diagnosis of Helicobacter pylori infection

McNicholl, Adrián G.; Ducóns, Julio; Barrio, Jesús; Bujanda, Luís; Forné-Bardera, Montserrat; Aparcero, R.; Ponce, Julio; Rivera, Robin; Dedeu-Cuso, José María; García-Iglesias, Pilar; Montoro, Miguel; Bejerano, Alicia; Nieto, Yolanda Ber; Madrigal, Belen; Zapata, Eva; Loras-Alastruey, Carmen; Castro, Manuel; Nevárez, Andrea; Méndez, Isabel; Bory-Ros, Felipe; Miquel-Planas, Mireia; Vera, Isabel; Nyssen, Olga P.; Gisbert, Javier P.

doi:10.1016/j.gastrohep.2017.07.007

Cited by 22 publications

(10 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As shown below, Vaira et al [25] did not find differences reading test results in 5 or in 60 minutes. McNichols et al [24] reported higher frequency of positive tests in a prospective series in cases read at 60 minutes regarding those read at 5 minutes (90% vs 78%), but these results came from different populations and settings, which do not allow their comparison. Along these lines we studied in our series the positive UFUT test read just before qPCR processing (between 24 hours to 2 weeks after biopsy), named as late UFUT (L-UFUT).…”

Section: Discussionmentioning

confidence: 93%

“…The RUT chosen in our study was the UFUT, sampling antrum and incisura angularis, and with reading after 5 minutes, following the manufacturer's instructions. In patients not selected by PPI consumption, the UFUT with reading at 30 and 60 minutes have demonstrated to be at least as accurate as conventional RUT with reading up to 24 hours [24]. Previously, Vaira et al [25] did not find differences between UFUT with reading at 60 and 5 minutes in specificity (100% in both) and sensitivity [96.2 (95%CI: 94.5-98) vs. 94.5 (95%CI: 92.4-96.6)], in this case for patients without prior PPI.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The Validity of the Invasive Tests for Helicobacter Pylori Diagnosis is Unequally Affected by the Consumption of Antibiotics or Pump Inhibitors. Test Performance under Real-World Conditions

Alcedo

Casas

Gotor

et al. 2021

JGLD

View full text Add to dashboard Cite

Background and Aims: Patients undergoing upper endoscopy have often used proton pump inhibitors (PPI) and/or antibiotics (ABx) recently. Both drugs have been associated with a poorer yield of the Helicobacter pylori (H. pylori) diagnostic tests. The aim was to assess the accuracy of the polymerase chain reaction test (qPCR), histological exam (HE) and ultra-fast urease test (UFUT) for H. pylori detection in patients that recently used PPI or ABx. Methods: Prospective study recruiting 206 patients who underwent upper endoscopy and gastric biopsies. Demographics and use of PPI/ABx were obtained. Sensibility (Sn), specificity (Sp), predictive value (PV), likelihood ratio (LR) and PABAK concordance index, were calculated, considering as the gold standard the positivity of 2 out of 3 analyzed tests. A global analysis and another one based on the PPI/ABx intake were performed. Results: 48.5% of patients used PPI and 12.8% ABx within the 2 and 4 weeks prior to endoscopy, respectively. The UFUT was positive in 13.1% of patients, HE in 34% and qPCR in 35.9%. UFUT achieved lower Sn (37%) than HE (98%) and qPCR (98%) (p<0.001) overall. ABx were associated with lower Sn in HE (p=0.04) and lower Sp in qPCR (p=0.03). PPI did not associate with a significant drop in Sn and Sp. The concordance between HE and qPCR was 0.83 (95%CI: 0.73-0.89). Conclusions: Under real world conditions, the accuracy and concordance of HE and qPCR to diagnose H. pylori were excellent, but UFUT achieved unsatisfactory outcomes. The intake of ABx was associated with the worse performance, fundamentally for HE. The PPI did not reduce the tests‘ yield significantly.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

The Validity of the Invasive Tests for Helicobacter Pylori Diagnosis is Unequally Affected by the Consumption of Antibiotics or Pump Inhibitors. Test Performance under Real-World Conditions

Alcedo

Casas

Gotor

et al. 2021

JGLD

View full text Add to dashboard Cite

show abstract

“…In contrast to these results, other authors reported higher percentages. MCNICHOLL et al (2017) (MCINTOSH et al, 2010). This difference in results showed a limitation of the technique.…”

Section: Resultsmentioning

confidence: 99%

Helicobacter spp. and gastric lesions association in minipigs

et al. 2021

View full text Add to dashboard Cite

Due to their similarity to humans, studies regarding gastric ulcers in pigs have become of great interest. It is known that Helicobacter spp. is related to the occurrence of these ulcers, as they possess a high prevalence in pigs. This study aimed to associate gastric lesions findings naturally occurring in minipigs related to or not related to the presence of Helicobacter spp., through the ultra-rapid urease test and immunohistochemical analysis, reaffirming the disease in swine as a natural biomedical model for human cases. For this, samples of formalin-fixed paraffin-embedded gastric tissues from 40 minipigs were obtained at the Laboratório de Morfologia e Patologia Animal (LMPA) in the Universidade Estadual do Norte Fluminense (UENF). Regarding the ultra-rapid urease test, no animal was positive in all regions. However, 18 were positive in at least one: six (15%) were positive in the aglandular region, eight (20%) in the antrum region, 13 (32.5%) in the region of the cardiac gland, and one (2.5%) in the region of the fundic gland. Regarding immunohistochemical analysis, only one animal was positive in all regions, and 32 animals were positive in at least one: four (10%) were positive in the aglandular region, 10 (25%) in the antrum region, 23 (57.5%) in the region of the cardiac gland, and three (7.5%) in the region of the fundic gland. The gastric lesion findings showed a close relationship with Helicobacter spp., enriching the laboratory animal pathologies list. The immunostaining of the bacteria not associated with gastric lesions in certain regions demonstrated the saprophytic and opportunisitic nature of Helicobacter.

show abstract

“…The method shows up to 82% of sensitivity and 90% of specificity. The main disadvantage is that RUT seems to decrease its relibility in case of proton pomps inhibitors use (Adu-Aryee et al, 2016;McNicholl et al, 2017;Dahlén et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Application of 16S rRNA gene sequencing in Helicobacter pylori detection

Szymczak

Ferenc

Majewska

et al. 2020

PeerJ

View full text Add to dashboard Cite

Helicobacter pylori is one of the major stomach microbiome components, promoting development of inflammation and gastric cancer in humans. H. pylori has a unique ability to transform into a coccoidal form which is difficult to detect by many diagnostic methods, such as urease activity detection, and even histopathological examination. Here we present a comparison of three methods for H. pylori identification: histological assessment (with eosin, hematoxylin, and Giemsa staining), polymerase chain reaction (PCR) detection of urease (ureA specific primers), and detection by 16S rRNA gene sequencing. The study employed biopsies from the antral part of the stomach (N = 40). All samples were assessed histologically which revealed H. pylori in eight patients. Bacterial DNA isolated from the bioptates was used as a template for PCR reaction and 16S rRNA gene sequencing that revealed H. pylori in 13 and in 20 patients, respectively. Thus, 16S rRNA gene sequencing was the most sensitive method for detection of H. pylori in stomach biopsy samples.

show abstract

Accuracy of the Ultra-Rapid Urease Test for diagnosis of Helicobacter pylori infection

Cited by 22 publications

References 15 publications

The Validity of the Invasive Tests for Helicobacter Pylori Diagnosis is Unequally Affected by the Consumption of Antibiotics or Pump Inhibitors. Test Performance under Real-World Conditions

The Validity of the Invasive Tests for Helicobacter Pylori Diagnosis is Unequally Affected by the Consumption of Antibiotics or Pump Inhibitors. Test Performance under Real-World Conditions

Helicobacter spp. and gastric lesions association in minipigs

Application of 16S rRNA gene sequencing in Helicobacter pylori detection

Contact Info

Product

Resources

About