Accuracy of two commercial enzyme-linked immunosorbent assays for the detection of antibodies to Salmonella spp. in slaughter pigs from Canada

Jaime, R. C. M.; Atashparvar, N.; Chirino‐Trejo, Manuel; Blasco, J.M.

doi:10.1016/j.prevetmed.2007.12.015

Cited by 11 publications

(13 citation statements)

References 33 publications

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“…However, Farzan and others (2007) described a significant disagreement at pig level between the latter two ELI SAs (kappa 0.56). In another publication, when HerdChek, with a cutoff OD value of 10 per cent, was compared with an ELI SA test not included in this study (Svanovir [Svanova Biotech]), there was moderate agreement (kappa 0.41) (Mainar-Jaime and others 2008). An international ring trial (in which a set of samples are sent to a number of laboratories for independent analysis and comparison) for porcine Salmonella ELI SAs was carried out in 2001 involving 12 laboratories using ‘in-house’ ELISAs or commercially available kits to test a set of 47 reference serum samples (van der Heijden and others 2001).…”

mentioning

confidence: 91%

Comparative study of three porcine Salmonella ELISAs

Poulin¹,

Donadeu

Mackay³

et al. 2010

Veterinary Record

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mentioning

confidence: 91%

Comparative study of three porcine Salmonella ELISAs

Poulin¹,

Donadeu

Mackay³

et al. 2010

Veterinary Record

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“…Differences could also be due to the lack of agreement showed between serology and microbiology at the pig level, especially when cut-off values of 10% or 20% were used (Nollet et al, 2005;Farzan et al, 2007;Mainar-Jaime et al, 2008b). We used a higher cut-off value (OD%≥40) that might not improve overall diagnostic accuracy but allowed higher confidence with regard to test specificity.…”

Section: Discussionmentioning

confidence: 99%

“…com/pubwebresources/pdf/en_us/livestock-poultry/ swine-salmonella-ab-test-sheet.pdf), test's sensitivity and specificity on field samples were estimated as 73% and 95%, respectively, in an independent study (Mainar-Jaime et al, 2008b). Nevertheless, in order to minimize the number of false-positive individual results a cut-off value OD% ≥ 40% (equivalent to a S/P ≥ 1) was used to deem an animal as positive.…”

Section: Serologymentioning

confidence: 99%

“…Although the detection of antibodies against Salmonella spp. is not the best indicator of infection at the pig level (Nollet et al, 2005;Mainar-Jaime et al, 2008b), most national programs against pig salmonellosis are based on serology as this technique is considered useful to establish the level of exposure of a herd and, therefore, some sort of "level of risk" (British Pig Executive, 2002;Alban et al, 2011;Anonymous, 2011b).…”

Section: Introductionmentioning

confidence: 99%

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Serological survey of Salmonella spp. infection in finishing pigs from northeastern Spain and associated risk factors

Vico

Jaime

2012

Span. j. agric. res.

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The results of a serological survey carried out in northeastern Spain to estimate the seroprevalence to Salmonella spp. and to determine potential risk factors are presented. Sera were obtained from farms submitting serum samples to the Regional Diagnostic Laboratory (RDL) for the diagnosis of other infectious diseases included within official eradication/surveillance programs, and farm data collected through a questionnaire. Out of 6,182 pig sera (217 farms) analyzed 1,219 (19.7%) were positive (optical density, OD ≥ 40%). More than 70% of the herds presented ≥ 1 seropositive animal indicating that salmonellosis was widespread. In a multivariable logistic mixed regression model seroprevalence was associated with farms where birds were visible inside the fattening units (OR = 2.1; 95% CI: 1.3-3.2) or that shared workers with other pig farms (OR = 2.5; 95% CI: 1.4-4.7). Seropositivity also increased when farmers used footwear exclusive for the farm (OR = 3.1; 95% CI: 1.3-7.3) or pigs were fed mostly with pellets (OR = 1.7; 95% CI: 1.1-2.6). It was also higher during the fall and winter months. The presence of a changing room and shower in the farm was negatively associated with it (OR = 0.5; 95% CI: 0.3-0.8). Most risk factors could be mitigated through strict hygiene and biosecurity measures, but the high (> 40%) within-herd seroprevalence observed in many herds may challenge the capacity of intervention of animal health authorities.Additional key words: ELISA test; risk factors; salmonellosis; seroprevalence; swine. ResumenEstudio serológico de la infección por Salmonella spp. en cerdos de engorde del Noreste de España y factores de riesgo asociados Se presentan los resultados de un estudio serológico realizado en explotaciones porcinas del Noreste de España para estimar la seroprevalencia frente a Salmonella spp. y determinar posibles factores de riesgo asociados. Los sueros se obtuvieron a partir de aquellos enviados por las explotaciones al Laboratorio Regional de Diagnóstico para el diagnóstico de otras enfermedades infecciosas sometidas a erradicación/vigilancia oficial, y los datos sobre las explotaciones se recogieron mediante un cuestionario. De un total de 6.182 sueros de cerdo (217 granjas) analizados 1.219 (19,7%) resultaron positivos (densidad óptica, DO ≥ 40%). Más del 70% de los rebaños presentaron al menos un animal positivo, indicando que la salmonelosis está ampliamente distribuida en la región. Según un modelo multivariable mixto de regresión logística, la seroprevalencia se asoció con granjas en las cuales se veían aves en el interior de las naves (OR = 2,1; IC 95%: 1,3-3,2) y también con granjas que compartían trabajadores (OR = 2,5; IC 95%: 1,4-4,7). Se observó que la seropositividad se incrementaba cuando los trabajadores usaban botas exclusivas en la explotación (OR = 3,1; IC 95%: 1,3-7,3) o al alimentar a los cerdos principalmente con alimento granulado (OR = 1,7; IC 95%: 1,1-2,6). La seroprevalencia también fue superior durante los meses de otoño e invierno. La existencia en las...

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“…; Mainar‐Jaime et al . ,b; Vico et al . ), high standards on farm biosecurity and hygiene are of utmost importance to prevent the contact of this pathogen with the live pig (De Busser et al .…”

Section: Introductionmentioning

confidence: 99%

Reduction of subclinical Salmonella infection in fattening pigs after dietary supplementation with a ß-galactomannan oligosaccharide

et al. 2014

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Aims: To assess the efficacy of a b-galactomannan oligosaccharide (ß-GMOS) for the control of Salmonella infection in fattening pigs. Methods and Results: Three different doses (0Á5, 3 and 2 kg ß-GMOS per ton of feed) were used during the entire period of growing in three similar and independent field trials carried out in a small fattening unit (%100 pigs). Treatment was randomly assigned to half of the pens. Individual serum samples (20-25 per group) were collected at different times during the fattening period and a similar number of faecal samples during the fattening period and at slaughter. In addition, mesenteric lymph nodes were collected at slaughter. Herdcheck â Swine Salmonella ELISA was used for serological analyses, the ISO 6579:2002/Amd 1 : 2007 for bacteriology and the PFGE for molecular characterization of Salmonella strains. The addition of ≥2 kg t À1 of ß-GMOS to the pig diet during the entire fattening period was associated with a reduction in Salmonella prevalence, shedding and seroconversion. Conclusions: Feed supplementation with ß-GMOS may be a useful complementary tool for the control of salmonellosis in fattening pigs. Significance and Impact of the Study: ß-GMOS may be a complementary way of reducing Salmonella shedding and infection in fattening pigs.

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Accuracy of two commercial enzyme-linked immunosorbent assays for the detection of antibodies to Salmonella spp. in slaughter pigs from Canada

Cited by 11 publications

References 33 publications

Comparative study of three porcine Salmonella ELISAs

Comparative study of three porcine Salmonella ELISAs

Serological survey of Salmonella spp. infection in finishing pigs from northeastern Spain and associated risk factors

Reduction of subclinical Salmonella infection in fattening pigs after dietary supplementation with a ß-galactomannan oligosaccharide

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