Accuracy of urine telomerase activity to detect bladder cancer in symptomatic patients

Casadio, Valentina; Bravaccini, Sara; Gunelli, Roberta; Nanni, Oriana; Zoli, Wainer; Amadori, Dino; Calistri, Daniele; Silvestrini, Rosella

doi:10.1177/172460080902400406

Cited by 11 publications

(10 citation statements)

References 23 publications

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“…Cytology test is very specific (a positive result is highly indicative of bladder cancer) but suffers from low sensitivity (a negative result does not exclude the diagnosis of cancer, particularly for cancer at first stages); comet test is able to identify early DNA damage that could increase the risk of cancer and TRAP analysis seems to be able to sensitively detect telomerase activity levels in superficial urothelial cell carcinoma [15, 18, 26]. …”

Section: Discussionmentioning

confidence: 99%

“…Other urinary biomarkers such telomerase activity (TA) levels by Telomeric repeat amplification protocol (TRAP) assay [18, 19], DNA damage in exfoliate urinary cells by comet assay [20], and DNA methylation markers are being investigated [16] and seem to be good candidates for early detection of bladder cancer. However these biomarkers require being validated in further studies on subjects at risk.…”

Section: Introductionmentioning

confidence: 99%

“…Many recent studies have also showed that telomerase activity quantification by TRAP assay in urine sediments, alone or in combination with a second level analysis, has an important role in distinguishing cancer patients from healthy individuals and also symptomatic patients [15, 18]. …”

Section: Introductionmentioning

confidence: 99%

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Assessment of DNA Damage and Telomerase Activity in Exfoliated Urinary Cells as Sensitive and Noninvasive Biomarkers for Early Diagnosis of Bladder Cancer in Ex-Workers of a Rubber Tyres Industry

Cavallo

Casadio

Bravaccini

et al. 2014

BioMed Research International

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The aim of the present study was to identify sensitive and noninvasive biomarkers of early carcinogenic effect at target organ to use in biomonitoring studies of workers at risk for previous occupational exposure to potential carcinogens. Standard urine cytology (Papanicolaou staining test), comet assay, and quantitative telomerase repeat amplification protocol (TRAP) assay were performed in 159 ex-rubber workers employed in tyres production and 97 unexposed subjects. In TRAP positive cases, a second level analysis using FISH (Urovysion) was done. Cystoscopy results were available for 11 individuals whose 6 FISH/TRAP/comet positive showed in 3 cases a dysplastic condition confirmed by biopsy, 1 comet positive resulted in infiltrating UBC to the biopsy and with hyperplasia and slight dysplasia to the urinary cytology, 1 comet positive resulted in papillary superficial UBC to the biopsy, 1 FISH/TRAP positive showed a normal condition, and 2 TRAP positive showed in one case a phlogosis condition. The results evidenced good concordance of TRAP, comet, and FISH assays as early biomarkers of procarcinogenic effect confirmed by the dysplastic condition and UBC found by cystoscopy-biopsy analysis. The analysis of these markers in urine cells could be potentially more accurate than conventional cytology in monitoring workers exposed to mixture of bladder potential carcinogens.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of DNA Damage and Telomerase Activity in Exfoliated Urinary Cells as Sensitive and Noninvasive Biomarkers for Early Diagnosis of Bladder Cancer in Ex-Workers of a Rubber Tyres Industry

Cavallo

Casadio

Bravaccini

et al. 2014

BioMed Research International

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“…

The first one, telomeric repeat amplification protocol (TRAP) assay , uses PCR amplification of a telomeric template with posterior analysis by ELISA or RT-PCR. This assay needs at least 50 copies of telomerase-expressing cells to have positive results, so the false negatives are something relatively frequent [47].

The second one, human telomerase reverse transcriptase (hTERT) assay , measures the messenger RNA levels of the catalytic subunit of the telomerase by RT-PCR [47].

…”

Section: Telomerase As Cancer Marker: Urine Biomarkermentioning

confidence: 99%

Urine Telomerase for Diagnosis and Surveillance of Bladder Cancer

Lamarca

Barriuso²

2012

Advances in Urology

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Bladder cancer has increased incidence during last decades. For those patients with nonmuscle involved tumors, noninvasive diagnosis test and surveillance methods must be designed to avoid current cystoscopies that nowadays are done regularly in a lot of patients. Novel urine biomarkers have been developed during last years. Telomerase is important in cancer biology, improving the division capacity of cancer cells. Even urinary telomerase could be a potentially useful urinary tumor marker; its use for diagnosis of asymptomatic and symptomatic patients or its impact during surveillance is still unknown. Moreover, there will need to be uniformity and standardization in the assays before it can become useful in clinical practice. It does not seem to exist a real difference between the most classical assays for the detection of urine telomerase (TRAP and hTERT). However, the new detection methods with modified TeloTAGGG telomerase or with gold nanoparticles must also be taken into consideration for the correct development of this diagnosis method. Maybe the target population would be the high-risk groups within screening programs. To date there is no enough evidence to use it alone and to eliminate cystoscopies from the diagnosis and surveillance of these patients. The combination with cytology or FISH is still preferred.

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“…Telomerase activity assessment in voided urine is an important noninvasive tool for detection, diagnosis and follow-up of human bladder cancer. Casadio et al verified that Telomerase activity assessment can detect tumor cells in urine with high sensitivity and specificity (Casadio et al, 2009). They evaluated the urinary telomerase activity of 515 patients, and found that the overall accuracy, in DOI:http://dx.doi.org/10.7314/APJCP.2012.13.5.1723 Potential Urinary Markers in the Early Detection, Diagnosis andFollow-up of Human Bladder Cancer terms of true positives and true negatives, was 80%.…”

Section: Telomerasementioning

confidence: 99%

Research Progress in Potential Urinary Markers for the Early Detection, Diagnosis and Follow-up of Human Bladder Cancer

Wang

Wang²

2012

Asian Pacific Journal of Cancer Prevention

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Objective: To summarize and evaluate various urinary markers for early detection, diagnosis and followup of human bladder cancer. Methods: A MEDLINE and PUBMED search of the latest literature on urinary markers for bladder cancer was performed. We reviewed these published reports and made a critical analysis. Results: Most urinary markers tend to be less specific than cytology, yielding more false-positive results, but demonstrating an advantage in terms of sensitivity, especially for detecting low grade, superficial tumors. Some tumor markers appear to be good candidates for early detection, diagnosis, and follow-up of human bladder cancer. Conclusion: A number of urinary markers are currently available that appear to be a applicable for clinical detection, diagnosis, and follow-up of bladder cancer. However, further studies are required to determine their accuracy and widespread applicability.

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Accuracy of urine telomerase activity to detect bladder cancer in symptomatic patients

Cited by 11 publications

References 23 publications

Assessment of DNA Damage and Telomerase Activity in Exfoliated Urinary Cells as Sensitive and Noninvasive Biomarkers for Early Diagnosis of Bladder Cancer in Ex-Workers of a Rubber Tyres Industry

Assessment of DNA Damage and Telomerase Activity in Exfoliated Urinary Cells as Sensitive and Noninvasive Biomarkers for Early Diagnosis of Bladder Cancer in Ex-Workers of a Rubber Tyres Industry

Urine Telomerase for Diagnosis and Surveillance of Bladder Cancer

Research Progress in Potential Urinary Markers for the Early Detection, Diagnosis and Follow-up of Human Bladder Cancer

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