Accurate assessment of in situ isometric contractile properties of hindlimb plantar and dorsal flexor muscle complex of intact mice

Gorselink, M.; Drost, Maarten R.; Louw, J. de; Willems, Paul; Rosielle, Nick; Janssen, JD Jan; Vusse, Ger J.

doi:10.1007/s004249900208

Cited by 15 publications

(20 citation statements)

References 12 publications

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“…The second law of motion (Eq. 1) on the coil plus shaft yields: (1) where g is the damping coefficient (N·m·s·rad -1 ), J the moment of inertia of coil and shaft (kg·m·s -2 ), k the stiffness due to the wire (N·m·rad -1 ), K o the torque offset (N·m), T f the friction torque (N·m), t the time (s), T ext the external torque, T motor the motor torque (N·m) and ϕ the shaft angle (rad)…”

Section: Methodsmentioning

confidence: 99%

“…The entire experimental apparatus consists of a thermostatic platform, hip fixation unit, the novel ergometer for assessment of the muscle-tendon complex properties during shortening and lengthening contractions and the isometric dynamometer for assessment of the contractile properties during isometric contractions [1]. For the sake of simplicity, the muscle-tendon complex will be termed "muscle complex" in the following.…”

Section: Methodsmentioning

confidence: 99%

“…Experimental procedures, such as fixation of the mouse and attachment of the electrode, have been described recently [1]. Briefly, the anaesthetised mouse was positioned on the thermostatic measurement platform and the foot was cast in a shoe made of two-component cement (Paladur, Heraus Kulzer, Wehrheim, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…With non-rigid fixation, displacement of the hind limb during the contraction will shorten the muscle-tendon complex and, hence, affect muscular performance through the force/length and force/velocity relationships [6,7,8]. Furthermore, we have shown previously that eccentricity between the ankle axis and the measurement shaft has only minor effects on contractile characteristics during isometric contraction [1]. Effects of eccentricity during shortening contractions have not been described, but theoretically, it may be assumed that eccentricity, particularly during shortening and lengthening contractions, will introduce an additional torque, due to inertial forces on the (lower) limbs or to forces on the knee fixation if a rigid knee fixation is used.…”

Section: Introductionmentioning

confidence: 95%

“…The availability of animal models with disrupted genes, encoding for proteins that are part of the contractile machinery or involved in energy conversion, has increased the need for small-scale measurement devices. Recently, we have developed an experimental device to assess in situ mechanical properties of isometric contractions of intact dorsal and plantar muscle complexes of the mouse [1]. Although this device provides valuable information on muscle mechanical performance, it is not appropriate for determining contractile properties during shortening and lengthening contractions of the murine hind limb ankle flexors.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

In situ assessment of shortening and lengthening contractile properties of hind limb ankle flexors in intact mice

Gorselink

Drost

Louw

et al. 2001

Pfl�gers Archiv European Journal of Physiology

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The availability of animal models with disrupted genes has increased the need for small-scale measurement devices. Recently, we developed an experimental device to assess in situ mechanical properties of isometric contractions of intact muscle complexes of the mouse. Although this apparatus provides valuable information on muscle mechanical performance, it is not appropriate for determining contractile properties during shortening and lengthening contractions. In the present study we therefore developed and evaluated an experimental apparatus for assessment of shortening and lengthening contractile properties of intact plantar and dorsal flexors of the mouse. The current through a custom-built, low-inertia servomotor was measured to assess contractile muscular torque ranging from -50 to mN.m. Evaluation of the fixation procedure of the animal to the apparatus via 3-D monitoring of the muscle-tendon complex length showed that the additional shortening in length due to a contraction with maximal torque output has only minor effects on the measured torque. Furthermore, misalignment of the axis of rotation of the apparatus relative to the axis of rotation in the ankle joint, i.e. eccentricity, during a routine experiment was estimated to be less than 1.0 mm and hence did not influence the measured torque output under our experimental conditions. Peak power per unit muscle mass (mean +/- SD) of intact dorsal and plantar flexors was 0.27 +/- 0.02 and 0.19 +/- 0.03 W.g-1, respectively. The angular velocity at maximal peak power generated by the dorsal flexor complex and the plantar flexor complex was 1100 +/- 190 and 700 +/- 90 degrees.s-1, respectively.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In situ assessment of shortening and lengthening contractile properties of hind limb ankle flexors in intact mice

Gorselink

Drost

Louw

et al. 2001

Pfl�gers Archiv European Journal of Physiology

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Determination of mouse skeletal muscle architecture using three‐dimensional diffusion tensor imaging

Heemskerk

Strijkers

Vilanova

et al. 2005

Magnetic Resonance in Med

145

142

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Muscle architecture is the main determinant of the mechanical behavior of skeletal muscles. This study explored the feasibility of diffusion tensor imaging (DTI) and fiber tracking to noninvasively determine the in vivo three-dimensional (3D) architecture of skeletal muscle in mouse hind leg. In six mice, the hindlimb was imaged with a diffusion-weighted (DW) 3D fast spin-echo (FSE) sequence followed by the acquisition of an exerciseinduced, T 2 -enhanced data set. The data showed the expected fiber organization, from which the physiological cross-sectional area (PCSA), fiber length, and pennation angle for the tibialis anterior (

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Impaired performance of skeletal muscle in α‐glucosidase knockout mice

et al. 2002

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Glycogen storage disease type II (GSD II) is an inherited progressive muscle disease in which lack of functional acid alpha-glucosidase (AGLU) results in lysosomal accumulation of glycogen. We report on the impact of a null mutation of the acid alpha-glucosidase gene (AGLU(-/-)) in mice on the force production capabilities, contractile mass, oxidative capacity, energy status, morphology, and desmin content of skeletal muscle. Muscle function was assessed in halothane-anesthetized animals, using a recently designed murine isometric dynamometer. Maximal torque production during single tetanic contraction was 50% lower in the knockout mice than in wild type. Loss of developed torque was found to be disproportionate to the 20% loss in muscle mass. During a series of supramaximal contraction, fatigue, expressed as percentile decline of developed torque, did not differ between AGLU(-/-) mice and age-matched controls. Muscle oxidative capacity, energy status, and protein content (normalized to either dry or wet weight) were not changed in knockout mice compared to control. Alterations in muscle cell morphology were clearly visible. Desmin content was increased, whereas alpha-actinin was not. As the decline in muscle mass is insufficient to explain the degree in decline of mechanical performance, we hypothesize that the large clusters of noncontractile material present in the cytoplasm hamper longitudinal force transmission, and hence muscle contractile function. The increase in muscular desmin content is most likely reflecting adaptations to altered intracellular force transmission.

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Accurate assessment of in situ isometric contractile properties of hindlimb plantar and dorsal flexor muscle complex of intact mice

Cited by 15 publications

References 12 publications

In situ assessment of shortening and lengthening contractile properties of hind limb ankle flexors in intact mice

In situ assessment of shortening and lengthening contractile properties of hind limb ankle flexors in intact mice

Determination of mouse skeletal muscle architecture using three‐dimensional diffusion tensor imaging

Impaired performance of skeletal muscle in α‐glucosidase knockout mice

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