Accurate determination of spring constant of atomic force microscope cantilevers and comparison with other methods

Kim, Min Seok; Choi, Jae-Hun; Kim, Jong Ho; Park, Yon Kyu

doi:10.1016/j.measurement.2009.12.020

Cited by 55 publications

(42 citation statements)

References 22 publications

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“…The spring constant of tips was calibrated by the thermal-noise method in a clean culture dish to each experiment [31], which were in the range of 0.3140 N/m-0.5083 N/m. Force curves were fit to the Sneddon model to estimate Young's modulus.…”

Section: Cck-8 Assaymentioning

confidence: 99%

A quantitative study of MC3T3-E1 cell adhesion, morphology and biomechanics on chitosan–collagen blend films at single cell level

Wang

Xie

Huang

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Section: Cck-8 Assaymentioning

confidence: 99%

A quantitative study of MC3T3-E1 cell adhesion, morphology and biomechanics on chitosan–collagen blend films at single cell level

Wang

Xie

Huang

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…This error is constant for all frequencies. The inverse method used for the calibration of the stiffer cantilever is also susceptible to errors greater than 10% (Kim et al, 2010). Another source of error arises from the measurement of the cantilever deflection.…”

Section: Atomic Force Microscopy (Afm)mentioning

confidence: 99%

Experimental methods for the characterization of the frequency-dependent viscoelastic properties of soft materials

Kazemirad

Heris

Mongeau

2013

The Journal of the Acoustical Society of America

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A characterization method based on Rayleigh wave propagation was developed for the quantification of the frequency-dependent viscoelastic properties of soft materials at high frequencies; i.e., up to 4 kHz. Planar harmonic surface waves were produced on the surface of silicone rubber samples. The phase and amplitude of the propagating waves were measured at different locations along the propagation direction, which allowed the calculation of the complex Rayleigh wavenumbers at each excitation frequency using a transfer function method. An inverse wave propagation problem was then solved to obtain the complex shear/elastic moduli from the measured wavenumbers. In a separate, related investigation, dynamic indentation tests using atomic force microscopy (AFM) were performed at frequencies up to 300 Hz. No systematic verification study is available for the AFM-based method, which can be used when the dimensions of the test samples are too small for other existing testing methods. The results obtained from the Rayleigh wave propagation and AFM-based indentation methods were compared with those from a well-established method, which involves the generation of standing longitudinal compression waves in rod-shaped test specimens. The results were cross validated and qualitatively confirmed theoretical expectations presented in the literature for the frequency-dependence of polymers.

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“…In other studies [36,37] it has been found that the Sader method underestimates the spring constant due to a rounding-off of the free end or from a non-rectangular cross section of real cantilevers. Our results for R-D, R-E and R-F tend to support this, with the fundamental spring constants determined by our method being 5-7% higher than those determined by the Sader method.…”

Section: Cantilever Dimensions and Shapementioning

confidence: 91%

Determination of the spring constants of the higher flexural modes of microcantilever sensors

Parkin¹,

Hähner²

2013

Nanotechnology

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Accurate determination of spring constant of atomic force microscope cantilevers and comparison with other methods

Cited by 55 publications

References 22 publications

A quantitative study of MC3T3-E1 cell adhesion, morphology and biomechanics on chitosan–collagen blend films at single cell level

A quantitative study of MC3T3-E1 cell adhesion, morphology and biomechanics on chitosan–collagen blend films at single cell level

Experimental methods for the characterization of the frequency-dependent viscoelastic properties of soft materials

Determination of the spring constants of the higher flexural modes of microcantilever sensors

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