Accurate flow in augmented networks (AFAN): an approach to generating three-dimensional biomimetic microfluidic networks with controlled flow

Keller, Keely A.; Govyadinov, Pavel A.; Ruchhoeft, Paul; Slater, John H.; Mayerich, David

doi:10.1039/c8ay01798k

Cited by 7 publications

(6 citation statements)

References 62 publications

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“…While there is substantial evidence that CTC endovascular attachment occurs frequently in capillaries and other microvessels during mouse tailvein injections due to physical en trapment and embolization (39), CTCs circulate through vessels of all sizes, which leaves open the possibility of CTC attachment events occurring in larger vessels; as does the demonstrations that CTCs, even as large clusters, can pass through small capillaries (40)(41)(42)(43). In addition, controlling the discrete geometries of endothelial capillary networks generated in vitro is currently not possible, making repro ducible studies of microvessel dynamics with respect to CTC behavior challenging (18). Here, we have sacrificed the most physiologically relevant vessel size for CTC colonization for the advantage of being able to generate a wide range of larger vessels in a reproducible manner to suit the systematic study of geometric effects.…”

Section: Discussionmentioning

confidence: 99%

“…While devices having selfassembled microvasculature are valuable tools for investigating mechanistic tumor cell and endothelial interactions in vitro (12)(13)(14)(15)(16)(17), the lack of control over the resulting geometries limits their potential as tools for specifically validating predictive threedimensional (3D) computational models. Recent work using a photolithographic technique to engineer microvesselscale chan nels has demonstrated the potential for reproducible and controlled design for investigating flow dynamics in capillary size channels with high geometric fidelity to in vivo through a synthetic hydrogel material for the purpose of validating a computational flow model (18). While this approach is an important method for examining physiological flow behaviors at this scale, it must trade off the unique ability to fabricate these channels with the inability to line these vessel proxies with a living endothelium, which may limit its potential for specifically examining CTCendothelium interactions in future studies.…”

Section: Introductionmentioning

confidence: 99%

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Examining metastatic behavior within 3D bioprinted vasculature for the validation of a 3D computational flow model

et al. 2020

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Understanding the dynamics of circulating tumor cell (CTC) behavior within the vasculature has remained an elusive goal in cancer biology. To elucidate the contribution of hydrodynamics in determining sites of CTC vascular colonization, the physical forces affecting these cells must be evaluated in a highly controlled manner. To this end, we have bioprinted endothelialized vascular beds and perfused these constructs with metastatic mammary gland cells under physiological flow rates. By pairing these in vitro devices with an advanced computational flow model, we found that the bioprinted analog was readily capable of evaluating the accuracy and integrated complexity of a computational flow model, while also highlighting the discrete contribution of hydrodynamics in vascular colonization. This intersection of these two technologies, bioprinting and computational simulation, is a key demonstration in the establishment of an experimentation pipeline for the understanding of complex biophysical events.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Examining metastatic behavior within 3D bioprinted vasculature for the validation of a 3D computational flow model

et al. 2020

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“…87 In a follow-up study, the same group has developed an automated method to design synthetic augmented channels to enforce controlled flow properties within the 3D networks. Using computational fluid dynamics methods, they demonstrated flow predictability that closely matches the experimental results, 88 opening a new door to create complex networks that mimic the in vivo counterparts.…”

Section: Laser-induced Hydrogel Degradationmentioning

confidence: 66%

Tissue Engineering and Regenerative Medicine 2019: The Role of Biofabrication—A Year in Review

Ramos

Moroni

2020

Tissue Engineering Part C: Methods

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Despite its relative youth, biofabrication is unceasingly expanding by assimilating the contributions from various disciplinary areas and their technological advances. Those developments have spawned the range of available options to produce structures with complex geometries while accurately manipulating and controlling cell behavior. As it evolves, biofabrication impacts other research fields, allowing the fabrication of tissue models of increased complexity that more closely resemble the dynamics of living tissue. The recent blooming and evolutions in biofabrication have opened new windows and perspectives that could aid the translational struggle in tissue engineering and regenerative medicine (TERM) applications. Based on similar methodologies applied in past years' reviews, we identified the most high-impact publications and reviewed the major concepts, findings, and research outcomes in the context of advancement beyond the state-of-the-art in the field. We first aim to clarify the confusion in terminology and concepts in biofabrication to therefore introduce the striking evolutions in three-dimensional and four-dimensional bioprinting of tissues. We conclude with a short discussion on the future outlooks for innovation that biofabrication could bring to TERM research.

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“…We have introduced a high-throughput imaging methodology for multiplex imaging of large-scale samples at sub-micrometer resolution at low cost. MUSE milling is capable of imaging denselyinterconnected microvascular networks, opening the door to simple acquisition and quantification of capillary changes common during disease progression [21] and guide the fabrication of in vitro disease models [22]. The proposed technique is compatible with a wide range of existing objectives, and can be integrated into immersionbased imaging systems to provide lateral resolution equivalent to existing fluorescence techniques.…”

Section: Discussion and Future Workmentioning

confidence: 99%

Three-Dimensional Microscopy by Milling with Ultraviolet Excitation

Artur

Eriksen

Mayerich

2019

Sci Rep

Self Cite

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Analysis of three-dimensional biological samples is critical to understanding tissue function and the mechanisms of disease. Many chronic conditions, like neurodegenerative diseases and cancers, correlate with complex tissue changes that are difficult to explore using two-dimensional histology. While three-dimensional techniques such as confocal and light-sheet microscopy are well-established, they are time consuming, require expensive instrumentation, and are limited to small tissue volumes. Three-dimensional microscopy is therefore impractical in clinical settings and often limited to core facilities at major research institutions. There would be a tremendous benefit to providing clinicians and researchers with the ability to routinely image large three-dimensional tissue volumes at cellular resolution. In this paper, we propose an imaging methodology that enables fast and inexpensive three-dimensional imaging that can be readily integrated into current histology pipelines. This method relies on block-face imaging of paraffin-embedded samples using deep-ultraviolet excitation. The imaged surface is then ablated to reveal the next tissue section for imaging. The final image stack is then aligned and reconstructed to provide tissue models that exceed the depth and resolution achievable with modern three-dimensional imaging systems.

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Accurate flow in augmented networks (AFAN): an approach to generating three-dimensional biomimetic microfluidic networks with controlled flow

Abstract: A network augmentation approach that adds synthetic connections to microvascular networks to induce biomimetic microfluidic flow.

Cited by 7 publications

References 62 publications

Examining metastatic behavior within 3D bioprinted vasculature for the validation of a 3D computational flow model

Examining metastatic behavior within 3D bioprinted vasculature for the validation of a 3D computational flow model

Tissue Engineering and Regenerative Medicine 2019: The Role of Biofabrication—A Year in Review

Three-Dimensional Microscopy by Milling with Ultraviolet Excitation

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