Accurate identification of Culicidae at aquatic developmental stages by MALDI-TOF MS profiling

Dieme, Constentin; Yssouf, Amina; Vega-Rúa, Anubis; Bérenger, Jean-Michel; Failloux, Anna‐Bella; Raoult, Didier; Parola, Philippe; Almeras, Lionel

doi:10.1186/preaccept-1955823754139029

Cited by 37 publications

(97 citation statements)

References 24 publications

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“…Among the mosquito storage modes tested here, the highest levels of MS mosquito species identification at both developmental stages were obtained for samples preserved frozen at -20ЊC (97.5% identification) or in liquid nitrogen (95.8% identification) for up to 6 months. These results are in compliance with previous studies using manual homogenization [20,26]. The frozen mode maintains MS spectra profiles for long periods of time.…”

Section: Discussionsupporting

confidence: 91%

“…Two automated grinding methods were tested using (ii) FastPrep-24 (MP Biomedicals Santa Ana, California, USA) and (iii) TissueLyser (Qiagen) devices. For these automated sample homogenization machines, three kinds of disruptors were tested including (iv) 3 mm tungsten Carbide beads (Qiagen), [23] and 20 L of 70% formic acid plus 20 L of 50% acetonitrile for L3 mosquito larvae [26]. The functions of the buffer are protein dissolution and extraction under acidic conditions to improve protein ionization [30].…”

Section: Sample Homogenizationmentioning

confidence: 99%

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Standardization of sample homogenization for mosquito identification using an innovative proteomic tool based on protein profiling

et al. 2016

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The rapid spread of vector-borne diseases demands the development of an innovative strategy for arthropod monitoring. The emergence of MALDI-TOF MS as a rapid, low-cost, and accurate tool for arthropod identification is revolutionizing medical entomology. However, as MS spectra from an arthropod can vary according to the body part selected, the sample homogenization method used and the mode and duration of sample storage, standardization of protocols is indispensable prior to the creation and sharing of an MS reference spectra database. In the present study, manual grinding of Anopheles gambiae Giles and Aedes albopictus mosquitoes at the adult and larval (L3) developmental stages was compared to automated homogenization. Settings for each homogenizer were optimized, and glass powder was found to be the best sample disruptor based on its ability to create reproducible and intense MS spectra. In addition, the suitability of common arthropod storage conditions for further MALDI-TOF MS analysis was kinetically evaluated. The conditions that best preserved samples for accurate species identification by MALDI-TOF MS were freezing at -20°C or in liquid nitrogen for up to 6 months. The optimized conditions were objectified based on the reproducibility and stability of species-specific MS profiles. The automation and standardization of mosquito sample preparation methods for MALDI-TOF MS analyses will popularize the use of this innovative tool for the rapid identification of arthropods with medical interest.

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Section: Discussionsupporting

confidence: 91%

Section: Sample Homogenizationmentioning

confidence: 99%

Standardization of sample homogenization for mosquito identification using an innovative proteomic tool based on protein profiling

et al. 2016

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“…software (Bruker Daltonics). DB1 included 915 MSPs from eight arthropod families, corresponding to the total MSPs of adult mosquito legs and larvae (Yssouf et al, 2013b(Yssouf et al, ,2014aDieme et al, 2014), tick legs and haemolymph (Yssouf et al, 2013a(Yssouf et al, , 2015a(Yssouf et al, , 2015b as well as sandfly and flea thoraces (Yssouf et al, 2014b;Lafri et al, 2016), which were principally homogenized manually. LSVs reflected MS spectral similarity with reference MS spectra from DB1.…”

Section: Spectra Analysis and Reference Database Upgradingmentioning

confidence: 99%

Comparative analysis of storage conditions and homogenization methods for tick and flea species for identification by MALDI‐TOF MS

Nebbak

Hamzaoui

Bérenger

et al. 2017

Medical Vet Entomology

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Ticks and fleas are vectors for numerous human and animal pathogens. Controlling them, which is important in combating such diseases, requires accurate identification, to distinguish between vector and non-vector species. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was applied to the rapid identification of arthropods. The growth of this promising tool, however, requires guidelines to be established. To this end, standardization protocols were applied to species of Rhipicephalus sanguineus (Ixodida: Ixodidae) Latreille and Ctenocephalides felis felis (Siphonaptera: Pulicidae) Bouché, including the automation of sample homogenization using two homogenizer devices, and varied sample preservation modes for a period of 1-6 months. The MS spectra were then compared with those obtained from manual pestle grinding, the standard homogenization method. Both automated methods generated intense, reproducible MS spectra from fresh specimens. Frozen storage methods appeared to represent the best preservation mode, for up to 6 months, while storage in ethanol is also possible, with some caveats for tick specimens. Carnoy's buffer, however, was shown to be less compatible with MS analysis for the purpose of identifying ticks or fleas. These standard protocols for MALDI-TOF MS arthropod identification should be complemented by additional MS spectrum quality controls, to generalize their use in monitoring arthropods of medical interest.

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“…Most tests of nonmorphological methods used for the identification of some parasitic arthropods always used the blind approach, comparing the results of a candidate method against the background of morphological identification by specialists (i.e. Dieme et al, 2014;Yssouf et al, 2013). Examples of this approach to ticks so far cover the use of DNA-barcoding for the detection of the blood meal source (Gariepy et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

A comparative test of ixodid tick identification by a network of European researchers

Estrada-Peña¹,

D’Amico

Palomar

et al. 2017

Ticks and Tick-borne Diseases

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This study reports the results of a comparative test of identification of ticks occurring in Western Europe and Northern Africa. A total of 14 laboratories were voluntarily enrolled in the test. Each participant received between 22 and 25 specimens of adult and nymphal ticks of 11 species: Dermacentor marginatus, D. reticulatus, Haemaphysalis punctata, Hyalomma lusitanicum, Hy. marginatum, Ixodes ricinus, I. hexagonus, Rhipicephalus annulatus, R. bursa, R. rossicus, and/or R. sanguineus s.l. Ticks were morphologically identified by three of the co-authors and the identification confirmed by a fourth co-author who used molecular methods based on several genes. Then ticks were randomly selected and blindly distributed among participants, together with a questionnaire. Only specimens collected while questing and, if possible, in the same survey, were circulated. Because of the random nature of the test, a participant could receive several specimens of the same species. Species in the different genera had variable misidentification rates (MR) of 7% (Dermacentor), 14% (Ixodes), 19% (Haemaphysalis), 36% (Hyalomma), and 54% (Rhipicephalus). Within genera, the MR was also variable ranging from 5.4% for I. ricinus or 7.4% for D. marginatus or D. reticulatus to 100% for R. rossicus. The test provided a total misidentification rate of 29.6% of the species of ticks. There are no significant differences in MR according to the sex of the tick. Participants were requested to perform a second round of identifications on the same set of ticks, using only purposely prepared keys (without illustrations), circulated to the enrolled participants, including 2 species of the genus Dermacentor, 8 of Haemaphysalis, 10 of Hyalomma, 23 of Ixodes, and 6 of Rhipicephalus. The average MR in the second round was 28%: 0% (Dermacentor), 33% (Haemaphysalis), 30% (Hyalomma) 18% (Ixodes), and 50% (Rhipicephalus). Species which are not reported in the countries of a participating laboratory had always highest MR, i.e. purely Mediterranean species had highest MR by laboratories in Central and Northern Europe. Participants expressed their concerns about a correct identification for almost 50% of the ticks of the genera Hyalomma and Rhipicephalus. The results revealed less than total confidence in identifying the most prominent species of ticks in the Western Palearctic, and underpin the need for reference libraries for specialists involved in this task. Results also showed that a combination of certain genes may adequately identify the target species of ticks. (Résumé d'auteur

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Accurate identification of Culicidae at aquatic developmental stages by MALDI-TOF MS profiling

Cited by 37 publications

References 24 publications

Standardization of sample homogenization for mosquito identification using an innovative proteomic tool based on protein profiling

Standardization of sample homogenization for mosquito identification using an innovative proteomic tool based on protein profiling

Comparative analysis of storage conditions and homogenization methods for tick and flea species for identification by MALDI‐TOF MS

A comparative test of ixodid tick identification by a network of European researchers

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