Accurate quantification of 5 German cockroach (GCr) allergens in complex extracts using multiple reaction monitoring mass spectrometry (MRM MS)

Mindaye, Samuel T.; Spiric, Jelena; David, Natalie A.; Rabin, Ronald L.; Slater, Jay E.

doi:10.1111/cea.12986

Cited by 17 publications

(29 citation statements)

References 37 publications

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“…LC–MS/MS is a rapidly developing technique and has raised bioanalysis to a new level. Multiple reaction monitoring (MRM) based on LC–MS/MS (LC–MRM) is a “gold standard” for the bioanalysis of small molecules [168–181]. MRM assay is a target MS technique based on ESI–MS [182–191].…”

Section: Bioanalytical Methods For Polyethylene Glycols and Pegylatedmentioning

confidence: 99%

Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals

Zhang

Song

et al. 2020

J of Separation Science

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Polyethylene glycols are synthetic polymers composed of repeating oxyethylene subunits, which have been known for non-toxic, non-immunogenic, non-antigenic, good solubility in water and therefore approved for pharmaceutical applications. Recently, attachment or amalgamation of polyethylene glycols to therapeutic small molecules, peptides, proteins, or nanoparticles has become a mature technology for the sake of improving their pharmacokinetic and pharmacological profiles, also referred to as PEGylation. By comparison, there are only a few PEGylated pharmaceuticals have been registered for further clinical trials and even less was approved for marketing. High failure rate of PEGylated pharmaceuticals in pre-clinical and clinical trials could be majorly attributed to their unclear pharmacokinetic behaviors. Therefore, the in vivo fate of the PEGylated pharmaceuticals for the various routes of administration needs to be thoroughly investigated An accurate in vivo pharmacological study thereof highly depends on the precise detection of polyethylene glycols as well as their fragments in biological matrixes. The goal of this review is to highlight the analytical methods that were developed and applied to evaluate the polyethylene glycols in pharmaceutical ingredients and excipients, which bring us closer to bridging

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Section: Bioanalytical Methods For Polyethylene Glycols and Pegylatedmentioning

confidence: 99%

Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals

Zhang

Song

et al. 2020

J of Separation Science

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“…169 Meanwhile, Polley and colleagues described how they looked for trypsin-like proteinases in German cockroach allergen extracts. 169 Meanwhile, Polley and colleagues described how they looked for trypsin-like proteinases in German cockroach allergen extracts.…”

Section: Cockroachmentioning

confidence: 99%

“…In 2017 Clinical and Experimental Allergy, Mindaye and colleagues described how liquid chromatography and multiple reaction monitoring mass spectrometry can be used to quantify five German cockroach allergens (Bla g 1, Bla g 2,Bla g 3, Bla g 4 and Bla g 5). 169 Meanwhile, Polley and colleagues described how they looked for trypsin-like proteinases in German cockroach allergen extracts. 170 Proteinases contribute to allergenicity and airway inflammation by activating proteinase-activated receptors.…”

Section: Cockroachmentioning

confidence: 99%

Developments in the field of allergy in 2017 through the eyes of Clinical and Experimental Allergy

Roberts

Almqvist

Boyle

et al. 2018

Clin Experimental Allergy

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“…The FDA identified GCr allergen extracts as an appropriate standardization target in 2001 10 . To achieve this goal, its efforts to measure the allergen content of these products have included ELISA, 6 ID 50 EAL, 11,12 a multiplex monoclonal antibody‐based approach 13 and physicochemical characterization 14 . The advantages of the last approach include increased sensitivity, accuracy, precision and linearity.…”

Section: Introductionmentioning

confidence: 99%

Measurement of German cockroach allergens and their isoforms in allergen extracts with mass spectrometry

Mindaye¹,

David²,

Esfahani³

et al. 2020

Clin Experimental Allergy

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Background: Allergen extracts are the primary tool for diagnosis and treatment of allergic diseases. In the United States, most allergen extracts are non-standardized.More sophisticated analytical approaches are needed to characterize these products and enable manufacturers and regulators to better determine potency.Objective: To expand the multiple reaction monitoring (MRM) assay for an in-depth characterization of German cockroach (GCr; Blattella germanica) allergen extracts. Methods:We applied advanced liquid chromatography (LC) and mass spectrometry (MS) techniques including MRM. The expanded LC/MRM-MS method was optimized to measure known GCr allergens and their isoforms/variants in commercial extracts and environmental samples. We performed isoform-specific allergen measurements in multiple extracts from four commercial sources and extracts prepared using environmental samples from urban homes. To investigate causes of heterogeneity, we examined over 30 extraction process variables.

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Accurate quantification of 5 German cockroach (GCr) allergens in complex extracts using multiple reaction monitoring mass spectrometry (MRM MS)

Cited by 17 publications

References 37 publications

Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals

Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals

Developments in the field of allergy in 2017 through the eyes of Clinical and Experimental Allergy

Measurement of German cockroach allergens and their isoforms in allergen extracts with mass spectrometry

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