Accurate quantification of dimethylamine (DMA) in human plasma and serum by GC–MS and GC–tandem MS as pentafluorobenzamide derivative in the positive-ion chemical ionization mode

Chobanyan, Kristine; Mitschke, Anja; Gutzki, Frank‐Mathias; Stichtenoth, Dirk O.; Tsikas, Dimitrios

doi:10.1016/j.jchromb.2007.03.006

Cited by 30 publications

(32 citation statements)

References 22 publications

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“…The DMA concentrations used in the validation experiment are relevant and were usually measured in liver homogenates after incubation with ADMA at final added concentrations of up to 100 M (see below). Thus, quantitative determination of DMA as pentafluorobenzamide derivative by the present GC-MS method seems to proceed with the same accuracy and precision in rat liver homogenate, human plasma [2] and human urine [3]. The internal standard d 6 -DMA was used in all experiments of the present work at a fixed concentration of 10 M, like in human plasma and serum [2], in order to cover the whole DMA concentration range.…”

Section: Intra-assay Precision and Accuracy Of The Methods For Dma Quamentioning

confidence: 97%

“…Because tissue homogenate in PBS resembles plasma or serum rather than urine with respect to protein concentration, DMA concentration expected to be formed, and pH, the derivatization/extraction procedure used in the present study is almost identical with that used for plasma and serum samples previously [2]. Samples were vortex-mixed manually using a Heidolph vortex mixer model Reax 2000 (Schwabach, Germany) at the highest vortex speed (i.e.…”

Section: Sample Derivatization and Extractionmentioning

confidence: 99%

“…Dimethylamine [DMA, (CH 3 ) 2 NH)] is present in human blood and urine [1][2][3]. Special attention has been paid to DMA since its identification as the hydrolysis product of N G ,N G -dimethyl-l-arginine (asymmetric dimethylarginine, ADMA) [4].…”

Section: Introductionmentioning

confidence: 99%

“…In previous work we have reported on GC-MS methods for the quantitative determination of DMA in human plasma and serum [2] and in urine [3]. These methods are based on the simultaneous derivatization of DMA by pentafluorobenzoyl chloride to its pentafluorobenzamide derivative and extraction into toluene (Fig.…”

Section: Introductionmentioning

confidence: 99%

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GC–MS assay for hepatic DDAH activity in diabetic and non-diabetic rats by measuring dimethylamine (DMA) formed from asymmetric dimethylarginine (ADMA): Evaluation of the importance of S-nitrosothiols as inhibitors of DDAH activity in vitro and in vivo in humans

Chobanyan

Thum

Suchy

et al. 2007

Journal of Chromatography B

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Section: Intra-assay Precision and Accuracy Of The Methods For Dma Quamentioning

confidence: 97%

Section: Sample Derivatization and Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

GC–MS assay for hepatic DDAH activity in diabetic and non-diabetic rats by measuring dimethylamine (DMA) formed from asymmetric dimethylarginine (ADMA): Evaluation of the importance of S-nitrosothiols as inhibitors of DDAH activity in vitro and in vivo in humans

Chobanyan

Thum

Suchy

et al. 2007

Journal of Chromatography B

Self Cite

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“…According to the literature, GC or GC-MS combined with extraction and derivatization was developed for the analysis of aliphatic amines in various samples. For example, Chobanyan et al developed pentafluorobenzoyl chloride derivatization coupled with solvent extraction for GC analysis of the dimethylamine in human blood [9]. Toro et al, Zhao et al and Mishra et al applied derivatization followed by solid-phase extraction and GC-MS to the analysis of aliphatic amines in water samples [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Development of single‐drop microextraction and simultaneous derivatization followed by GC‐MS for the determination of aliphatic amines in tobacco

Sha

Meng

Lin

et al. 2010

J of Separation Science

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In this work, for the first time, headspace (HS) single-drop microextraction and simultaneous derivatization followed by GC-MS was developed to determine the aliphatic amines in tobacco samples. In the HS extraction procedure, the mixture of derivatization reagent and organic solvent was employed as the extraction solvent for HS single-drop microextraction and in situ derivatization of aliphatic amine in the samples. Fast extraction and simultaneous derivatization of the analytes were performed in a single step, and the obtained derivatives in the microdrop extraction solvent were analyzed by GC-MS. The optimized experiment conditions were: sample preparation temperature of 80 degrees C and time of 30 min, HS extraction solvent (the mixture of benzyl alcohol and 2,3,4,5,6-pentafluorobenzaldehyde) volume of 2.0 microL, extraction time of 90 s. With the optimal conditions, the method validations were also studied. The method has good linearity (R(2) more than 0.99), accepted precision (RSD less than 13%), good recovery (98-104%) and low limit of detection (0.11-0.97 microg/g). Finally, the proposed technique was successfully applied to the analyses of aliphatic amines in tobacco samples of seven different brands. It was further demonstrated that the proposed method offered a simple, low-cost and reliable approach to determine aliphatic amines in tobacco samples.

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Novel Flow‐through Potentiometric System for Dimethyamine Assessment Using New Ion Exchangers Doped‐polymeric Membrane Sensors

2018

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New simple potentiometric sensing systems are described for the sensitive and selective monitoring of dimethylamine (DMA). These systems are based on using polymeric membranes doped in some ion exchangers such as dimethylamine/phosphomolybdate [(CH3)2NH3]22+[MP]2− (Sensor I), dimethylamine/tetraphenylborate [(CH3)2NH3]+[TPB]− (Sensor II) and dimethylamine/phosphotungstate [(CH3)2NH3]+[TP]− (sensor III). All the sensing systems are incorporated in poly(vinyl chloride) matrix plasticized with o‐NPOE. In a Trizma buffer solution of pH 7.1, the sensors exhibit a stable, fast and linear response for 5.0×10−6–1.0×10−2, 2.5×10−5–1.0×10−2, and 5.0×10−6–1.0×10−2 mol L−1 DMA+ with slopes of 53.2±0.4, 49.4±0.6, and 53.8±0.3 mV decade−1, and detection limits of 2.0×10−6 (0.16 μg mL−1), 1.6×10−5(1.3 μg mL−1), and 1.4×10−6 (0.11 μg mL−1) mol L−1 for sensors (I), (II), and (III), respectively. Potentiometric selectivity measurements of these sensors reveal negligible interferences from most common aliphatic and aromatic amines. Satisfactory results are obtained with an average accuracy of 99.5 % and a mean precision of ±0.5 %. Validation of the proposed assay methods are examined by measuring the limit of detection, linearity range, accuracy, precision, within‐day repeatability, between‐days reproducibility and method ruggedness. Good performance characteristics and applicability of these sensors for DMA assessment in different matrices under static and hydrodynamic modes of measurements are demonstrated. The proposed sensors are also prepared in a tubular form and used as DMA detector in a two‐channel flow injection setup for continuous monitoring. The frequency rate is 27–53 samples h−1. The sensors are satisfactory applied for DMA quantification in both biological matrices and environmental samples with recoveries ranging between 95.0 and 104.0 %.

show abstract

Accurate quantification of dimethylamine (DMA) in human plasma and serum by GC–MS and GC–tandem MS as pentafluorobenzamide derivative in the positive-ion chemical ionization mode

Cited by 30 publications

References 22 publications

GC–MS assay for hepatic DDAH activity in diabetic and non-diabetic rats by measuring dimethylamine (DMA) formed from asymmetric dimethylarginine (ADMA): Evaluation of the importance of S-nitrosothiols as inhibitors of DDAH activity in vitro and in vivo in humans

GC–MS assay for hepatic DDAH activity in diabetic and non-diabetic rats by measuring dimethylamine (DMA) formed from asymmetric dimethylarginine (ADMA): Evaluation of the importance of S-nitrosothiols as inhibitors of DDAH activity in vitro and in vivo in humans

Development of single‐drop microextraction and simultaneous derivatization followed by GC‐MS for the determination of aliphatic amines in tobacco

Novel Flow‐through Potentiometric System for Dimethyamine Assessment Using New Ion Exchangers Doped‐polymeric Membrane Sensors

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