ACE1 regulates expression of the Saccharomyces cerevisiae metallothionein gene.

Thiele, Dennis J.

doi:10.1128/mcb.8.7.2745

Cited by 287 publications

(257 citation statements)

References 33 publications

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“…In the budding yeast S. cerevisiae, metallothionein is encoded for by two genes: CUP1 and CRS5 (Culotta et al, 1994). CUP1 expression is highly induced in response to copper exposure, mediated by the Ace1 transcription factor, and is clearly important in the prevention of toxicity towards copper (Ecker et al, 1986;Thiele, 1988). The role of Crs5 is less clearly defined, although it does play some role in regulating metal ion homeostasis (Culotta et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

The adaptive response of Saccharomyces cerevisiae to mercury exposure

Westwater

McLaren

Dormer

et al. 2002

Yeast

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The budding yeast Saccharomyces cerevisiae has been shown to possess a number of discrete but overlapping adaptive stress responses. We show here that yeast has an adaptive stress response towards mercury and that this response overlaps to some extent with the H 2 O 2 and cadmium-inducible stress responses. Expression of the yeast GSH1 gene, encoding c-glutamylcysteine synthetase, is known to be regulated by hydrogen peroxide; in this study we show that expression of a GSH1-lacZ reporter gene is shown to be regulated by exposure to heavy metals, such as mercury and cadmium. Other redoxactive metals, including copper and iron, were found not to induce GSH1 expression. We show that mercury-mediated regulation of the GSH1 gene is not by the same mechanism used by cadmium. Moreover, our experiments suggest the possibility that the oxidative stress produced by mercury exposure is similar to that produced by treatment with H 2 O 2 , consistent with our finding that the Yap1 protein is also involved in the response of yeast towards mercury.

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Section: Introductionmentioning

confidence: 99%

The adaptive response of Saccharomyces cerevisiae to mercury exposure

Westwater

McLaren

Dormer

et al. 2002

Yeast

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“…The mechanism by which copper ions induce transcription of the Cu-MT gene has received detailed attention (Thiele & Hamer, 1986;Butt & Ecker, 1987;Thiele, 1988;Furst et al, 1988;Furst & Hamer, 1989;Culotta et al, 1989;Welch et al, 1989;Hu, Furst & Hamer, 1990). It is now known that a ^raw^-acting regulatory protein, encoded by the ACE 1 locus (Thiele, 1988;Furstetal, 1988) and also known as the CUP2 locus Buchman et al, 1989), activates transcription of the Cu-MT gene in response to excess copper (or silver) ions .…”

Section: Intracellular Fate Of Toxic Metals* (A) Metal-binding Proteimentioning

confidence: 99%

“…It is now known that a ^raw^-acting regulatory protein, encoded by the ACE 1 locus (Thiele, 1988;Furstetal, 1988) and also known as the CUP2 locus Buchman et al, 1989), activates transcription of the Cu-MT gene in response to excess copper (or silver) ions . Although the ACEl gene is constitutively expressed in the absence or presence of copper, the apoprotein cannot bind DNA (Fiirst et al, 1988).…”

Section: Intracellular Fate Of Toxic Metals* (A) Metal-binding Proteimentioning

confidence: 99%

Interactions of fungi with toxic metals

1993

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SUMMARY This review details the major interactions of fungi with toxic metal species. Physico‐chemical and biological aspects are discussed including definitions and classification of metals in biological contexts, mechanisms of metal fungitoxicity, resistance and tolerance, environmental influences and the occurrence of fungi in metal‐polluted habitats. Cellular interactions include extracellular precipitation and complexation, binding to cell walls, transport of monovalent and divalent metal cations, intracellular fates of toxic metal species (including metal‐binding proteins and peptides, and vacuolar compartmentation), and metal transformations including organometal(loid) synthesis. Significant interactions between metals and mycorrhizal fungi and macrofungi are summarized with reference to the amelioration of plant phytotoxicity and metal accumulation respectively. Biotechnological aspects of metal‐fungal interactions relate to the biological treatment of metal‐contaminated effluents and waste streams for metal removal/recovery and environmental protection.

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“…Whereas unicellular eukaryotes, such as yeast, have evolved their own metal-scavenging system (Thiele, 1988;Liu and Thiele, 1997) and while the process of metallothionein gene activation in the nematode worm C. elegans has remained elusive, MTF-1 is conserved in vertebrates and insects (Radtke et al, 1993;Brugnera et al, 1994;Auf der Maur et al, 1999;Dalton et al, 2000;Zhang et al, 2001). We have recently identified and characterized dMTF-1, the MTF-1 homolog of the fruit fly Drosophila (Zhang et al, 2001;Egli et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Metal-responsive transcription factor (MTF-1) and heavy metal stress response in Drosophila and mammalian cells: a functional comparison

Balamurugan

Egli²,

Selvaraj³

et al. 2004

Biological Chemistry

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The zinc finger transcription factor MTF-1 (metal-responsive transcription factor-1) is conserved from insects to vertebrates. Its major role in both organisms is to control the transcription of genes involved in the homeostasis and detoxification of heavy metal ions such as Cu 2q , Zn 2q and Cd 2q . In mammals, MTF-1 serves at least two additional roles. First, targeted disruption of the MTF-1 gene results in death at embryonic day 14 due to liver degeneration, revealing a stage-specific developmental role. Second, under hypoxic-anoxic stress, MTF-1 helps to activate the transcription of the gene placental growth factor (PlGF), an angiogenic protein. Recently we characterized dMTF-1, the Drosophila homolog of mammalian MTF-1. Here we present a series of studies to compare the metal response in mammals and insects, which reveal common features but also differences. A human MTF-1 transgene can restore to a large extent metal tolerance to flies lacking their own MTF-1 gene, both at low and high copper concentrations. Likewise, Drosophila MTF-1 can substitute for human MTF-1 in mammalian cell culture, although both the basal and the metal-induced transcript levels are lower. Finally, a clear difference was revealed in the response to mercury, a highly toxic heavy metal: metallothionein-type promoters respond poorly, if at all, to Hg 2q in mammalian cells but strongly in Drosophila, and this response is completely dependent on dMTF-1.

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ACE1 regulates expression of the Saccharomyces cerevisiae metallothionein gene.

Cited by 287 publications

References 33 publications

The adaptive response of Saccharomyces cerevisiae to mercury exposure

The adaptive response of Saccharomyces cerevisiae to mercury exposure

Interactions of fungi with toxic metals

Metal-responsive transcription factor (MTF-1) and heavy metal stress response in Drosophila and mammalian cells: a functional comparison

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