Acellular pertussis vaccination facilitates<i>Bordetella parapertussis</i>infection in a rodent model of bordetellosis

Long, Gráinne H.; Karanikas, Alexia T.; Harvill, Eric T.; Read, Andrew F.; Hudson, Peter J.

doi:10.1098/rspb.2010.0010

Cited by 33 publications

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“…Whooping cough vaccines are still derived solely from B. pertussis. These vaccines were found to be less protective against B. parapertussis (8,10,40), eventually leading to a selective advantage of B. parapertussis over B. pertussis (3,13,17,18). Accordingly, B. parapertussis has been found to cause larger proportions of whooping cough cases than before among vaccinated groups, with a significant increase in prevalence after the introduction of the acellular vaccines (4,17,19,35).…”

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confidence: 99%

Bordetella parapertussis Survives the Innate Interaction with Human Neutrophils by Impairing Bactericidal Trafficking inside the Cell through a Lipid Raft-Dependent Mechanism Mediated by the Lipopolysaccharide O Antigen

et al. 2012

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Bordetella parapertussis Survives the Innate Interaction with Human Neutrophils by Impairing Bactericidal Trafficking inside the Cell through a Lipid Raft-Dependent Mechanism Mediated by the Lipopolysaccharide O Antigen

et al. 2012

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“…Current whooping cough vaccines do not induce antibodies against B. parapertussis (6,8,9,44,45). The lipopolysaccharide (LPS) O antigen was found to be implicated in the lack of protection of pertussis vaccines against this pathogen by interfering with the binding of antibodies induced by vaccine antigens common to both species (9,10).…”

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confidence: 99%

Bordetella parapertussis Survives inside Human Macrophages in Lipid Raft-Enriched Phagosomes

2014

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bBordetella parapertussis is a human pathogen that causes whooping cough. The increasing incidence of B. parapertussis has been attributed to the lack of cross protection induced by pertussis vaccines. It was previously shown that B. parapertussis is able to avoid bacterial killing by polymorphonuclear leukocytes (PMN) if specific opsonic antibodies are not present at the site of interaction. Here, we evaluated the outcome of B. parapertussis innate interaction with human macrophages, a less aggressive type of cell and a known reservoir of many persistent pathogens. The results showed that in the absence of opsonins, O antigen allows B. parapertussis to inhibit phagolysosomal fusion and to remain alive inside macrophages. The O antigen targets B. parapertussis to lipid rafts that are retained in the membrane of phagosomes that do not undergo lysosomal maturation. Fortyeight hours after infection, wild-type B. parapertussis bacteria but not the O antigen-deficient mutants were found colocalizing with lipid rafts and alive in nonacidic compartments. Taken together, our data suggest that in the absence of opsonic antibodies, B. parapertussis survives inside macrophages by preventing phagolysosomal maturation in a lipid raft-and O antigen-dependent manner. Two days after infection, about 15% of macrophages were found loaded with live bacteria inside flotillin-enriched phagosomes that had access to nutrients provided by the host cell recycling pathway, suggesting the development of an intracellular infection. IgG opsonization drastically changed this interaction, inducing efficient bacterial killing. These results highlight the need for B. parapertussis opsonic antibodies to induce bacterial clearance and prevent the eventual establishment of cellular reservoirs of this pathogen. Bordetella parapertussis and Bordetella pertussis are human pathogens that cause whooping cough, a reemerging disease that remains a threat to human health. Despite high vaccination coverage, whooping cough is still endemic. Current clinical surveys indicate that B. parapertussis is responsible for a significant number of cases of whooping cough, particularly in vaccinated populations (1-5). The switch from whole-cell to acellular vaccines is associated with a significant increase in the prevalence of B. parapertussis in the epidemiology of the disease (6, 7). Several studies have demonstrated that pertussis acellular vaccines fail to protect against B. parapertussis (6, 8). The lack of cross protection was mainly attributed to the presence of the O antigen on the surface of B. parapertussis, which blocks antibody access to the vaccine antigens common to both species (9, 10). In vitro studies confirmed that pertussis acellular vaccines induce antibodies that opsonize B. pertussis but not B. parapertussis (9, 10). In the absence of opsonic antibodies, B. parapertussis survives neutrophil phagocytosis by preventing lysosomal maturation in a lipid raft-dependent manner (11). O antigen is involved in this nonbactericidal interaction, mediating t...

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“…Some authors have postulated that vaccination with aPs can interfere with the "clearance" of B. parapertussis, facilitating the adaptive performance of this pathogen, which could lead to the emergence of more susceptible hosts to B. parapertussis infection [92]. Accordingly, a gradual increase in the prevalence of B. parapertussis has been observed as a result of epidemiological pertussis immunization with vaccines that are less protective against B. parapertussis than the natural infection with B. pertussis [93].…”

Section: Pertussis Resurgence: a Multifactorial Problemmentioning

confidence: 99%

Immunization against Pertussis: An Almost Solved Problem or a Headache in Public Health

Dias¹,

Prestes²,

Cunegundes³

et al. 2017

Vaccines

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Whooping cough or pertussis is a serious infectious disease of the human respiratory tract, caused by Gram-negative bacteria Bordetella pertussis and Bordetella parapertussis HU . The current pertussis vaccines may consist of dead cells of B. pertussis (whole cell pertussis vaccines-wPs) or purified antigens from the bacterium (acellular pertussis vaccines-aPs). The aPs are less reactogenic and have been widely used in developed countries for more than two decades, but their high cost of production makes them prohibitive for developing countries, and the accelerated rate of epidemic outbreaks has led to the hypothesis that aPs are less effective than the wP ones. Considering cost-effectiveness, some authors have pointed out questions about the possibility of reintroduction of wP vaccines into the primary doses of pertussis vaccination. The Butantan Institute in São Paulo, Brazil, developed a wP vaccine with low endotoxicity (Plow) obtained by chemical extraction of the lipooligosaccharide (LOS) fraction from the outer membrane of the bacterial cell, showing to be less reactogenic and equally immunogenic and protective as the traditional wP vaccine. The Plow may possibly be introduced into the vaccination schedule for immunization of adolescents and young adults in Brazil, an important epidemiological contribution to reducing the circulation of B. pertussis.

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Acellular pertussis vaccination facilitatesBordetella parapertussisinfection in a rodent model of bordetellosis

Cited by 33 publications

References 47 publications

Bordetella parapertussis Survives the Innate Interaction with Human Neutrophils by Impairing Bactericidal Trafficking inside the Cell through a Lipid Raft-Dependent Mechanism Mediated by the Lipopolysaccharide O Antigen

Bordetella parapertussis Survives the Innate Interaction with Human Neutrophils by Impairing Bactericidal Trafficking inside the Cell through a Lipid Raft-Dependent Mechanism Mediated by the Lipopolysaccharide O Antigen

Bordetella parapertussis Survives inside Human Macrophages in Lipid Raft-Enriched Phagosomes

Immunization against Pertussis: An Almost Solved Problem or a Headache in Public Health

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