Acemannan increases NF-κB/DNA binding and IL-6/-8 expression by selectively binding Toll-like receptor-5 in human gingival fibroblasts

Thunyakitpisal, Pasutha; Ruangpornvisuti, Vithaya; Kengkwasing, Pattrawadee; Chokboribal, Jaroenporn; Sangvanich, Polkit

doi:10.1016/j.carbpol.2016.12.034

Cited by 40 publications

(29 citation statements)

References 61 publications

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“…Another study has shown that compounds of aloe species inhibit the production of pro-inflammatory cytokines and nitric oxide via inhibition of the MAPK signalling pathway [15]. More studies proved that treatment of aloin decreased the high production of interleukin (IL)-8 induced by IL-1β in KB cells, through the inhibition of activity of p38 and extracellular signal-regulated kinases pathway [19,29]. In recent years, more and more researchers have found that oxidative stress promotes the secondary injury after TBI, and among multiple factors, nicotinamide adenine dinucleotide phosphate oxidase (NOX) is critical in regulating the production of ROS [1].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of NOX2 contributes to the therapeutic effect of aloin on traumatic brain injury

Dong¹,

Wang²,

Zhang³

2020

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Traumatic brain injury (TBI) is a subset of brain injury induced by external mechanical forces to the head or neck. TBI has been reported to be one of the leading causes of disability, and it causes a huge financial burden around the world. Aloin is the major anthraquinone glycoside extracted from Aloe species, and has presented anti-tumour, antioxidative and anti-inflammatory activities. However, few studies have focused the effect of aloin in treatment of TBI. Nicotinamide adenine dinucleotide phosphate oxidase (NOX) is the only subset of enzymes which produces solely the reactive oxygen species (ROS). A recent study showed that activation of NOX might aggravate the primary TBI, and among these members, NOX2 is the key member in regulation of uncontrolled ROS expression, and thus plays a critical role in development of inflammatory diseases. Here, we noticed that inhibition of NOX2 combined with aloin treatment promoted the recovery of brain function in a mice model as well as the viability rate in a cell model. A further study found that the inflammation response process was also inhibited after treatment. Then, we found that these effects might be mediated by the PI3K/AKT/mTOR signalling pathway and NOX2 might be a therapeutic target for TBI.

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Section: Introductionmentioning

confidence: 99%

Inhibition of NOX2 contributes to the therapeutic effect of aloin on traumatic brain injury

Dong¹,

Wang²,

Zhang³

2020

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“…The acetyl group located on the mannose residue has been proposed as an acemannan functional domain. The bioactivity of acemannan comes from its 3D intramolecular and intermolecular architecture rather than the building block monosaccharides [14,31]. Although the regenerative mechanism of acemannan has not been explicitly identified and analyzed, prior studies demonstrated that acemannan induced pulp healing and dentin formation in vitro and in vivo [10,13].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, acemannan has immunomodulatory activity. Selectively binding to TLR5, acemannan enhanced NF-κB DNA binding activity, and upregulated proinflammatory cytokine IL-6 and -8 expression [14]. Therefore, acemannan might hasten the progression from the inflammatory phase to the formative phase of tissue regeneration.…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, Aloe vera pulp gel (Aloe barbadensis Miller) was obtained from mature leaves. After homogenization, centrifugation, precipitation with alcohol, and lyophilization, the white acemannan precipitate was characterized by 1H-NMR, 13C-NMR (Fourier 300, Bruker, Ettlingen, Baden-Württemberg, Germany), and FT-IR (Spectrum System 2000; PerkinElmer, Waltham, MA, USA) [14].…”

Section: Acemannan Sponge Preparationmentioning

confidence: 99%

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Acemannan Used as an Implantable Biomaterial for Vital Pulp Therapy of Immature Permanent Teeth Induced Continued Root Formation

Nguyen

Sangvanich

et al. 2020

Pharmaceutics

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Direct pulp-capping, a vital pulp therapy, is used to protect and preserve pulp vitality by applying a biomaterial on the pulp exposure site. Acemannan, a polysaccharide extracted from Aloe vera, induces osteodentin-bridge formation to cover the exposure site in vivo. We evaluated the effect of acemannan sponges on partial pulpotomized permanent teeth with caries or accident-induced pulp exposure (n = 50). After removing infected dentin and inflamed pulp tissue, the teeth were randomly divided into acemannan or control (mineral trioxide aggregate (MTA) groups (n = 25). The teeth were examined immediately after treatment (baseline) and at 6- and 12-month follow-ups for clinical and cone beam computed tomography (CBCT) examination. The three-dimensional tooth length and root apex area were simulated to determine treatment success. We found that the overall success rate in the acemannan and MTA groups from baseline to 12-month follow-up was 90.91% and 95.65%, respectively, with no significant difference between the two groups (p > 0.05). In the success teeth in both groups, the root length increased, and the apex area significantly decreased (p < 0.05), indicating continued root formation. Our results suggest that acemannan is a promising low-cost biomaterial for partial pulpotomy treatment for immature permanent teeth requiring vital pulp therapy.

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“…ACM has been shown to have immunomodulatory activities ( 19 ). It has been reported to induce maturation of dendritic cells, which are important in the activation of T cell responses ( 35 ); to enhance the generation of cytotoxic T-cells in response to an alloantigen ( 36 ); and to upregulate cytokine production in different model systems ( 19 37 ). Taken together, these previous reports suggest mechanisms through which ACM enhances the immunogenicity of the influenza vaccine.…”

Section: Discussionmentioning

confidence: 99%

Adjuvanticity of Processed Aloe vera gel for Influenza Vaccination in Mice

et al. 2020

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The effectiveness of current influenza vaccines is considered suboptimal, and 1 way to improve the vaccines is using adjuvants. However, the current pool of adjuvants used in influenza vaccination is limited due to safety concerns. Aloe vera, or aloe, has been shown to have immunomodulatory functions and to be safe for oral intake. In this study, we explored the potential of orally administered processed Aloe vera gel (PAG) as an adjuvant for influenza vaccines in C57BL/6 mice. We first evaluated its adjuvanticity with a split-type pandemic H1N1 (pH1N1) Ag by subjecting the mice to lethal homologous influenza challenge. Oral PAG administration with the pH1N1 Ag increased survival rates in mice to levels similar to those of alum and MF59, which are currently used as adjuvants in influenza vaccine formulations. Similarly, oral PAG administration improved the survival of mice immunized with a commercial trivalent influenza vaccine against lethal homologous and heterologous virus challenge. PAG also increased hemagglutination inhibition and virus neutralization Ab titers against homologous and heterologous influenza strains following immunization with the split-type pH1N1 Ag or the commercial trivalent vaccine. Therefore, this study demonstrates that PAG may potentially be used as an adjuvant for influenza vaccines.

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Acemannan increases NF-κB/DNA binding and IL-6/-8 expression by selectively binding Toll-like receptor-5 in human gingival fibroblasts

Cited by 40 publications

References 61 publications

Inhibition of NOX2 contributes to the therapeutic effect of aloin on traumatic brain injury

Inhibition of NOX2 contributes to the therapeutic effect of aloin on traumatic brain injury

Acemannan Used as an Implantable Biomaterial for Vital Pulp Therapy of Immature Permanent Teeth Induced Continued Root Formation

Adjuvanticity of Processed Aloe vera gel for Influenza Vaccination in Mice

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