Acentromeric micronuclei are increased in peripheral blood lymphocytes of untreated cancer patients

Baciuchka-Palmaro, M.; Orsière, Thierry; Duffaud, Florence; Sari-Minodier, I.; Pompili, J.; Bellon, L.; Méo, Michel De; Digue, Laurence; Favre, R.; Botta, A.

doi:10.1016/s1383-5718(02)00206-1

Cited by 36 publications

(11 citation statements)

References 48 publications

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“…In fact, Del Rey et al [18] suggested that MN resulting from acentric fragments (chromosome breakage or cyclin D1 gene amplification) or from whole chromosomes might contribute to genomic instability in UCC tumor samples. Similarly, a high frequency of acentromeric MN was observed in the peripheral lymphocytes of patients with different types of cancer prior to treatment [11].…”

Section: Discussionmentioning

confidence: 76%

Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma

et al. 2014

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Bladder cancer is one of the most common genitourinary neoplasms in industrialized countries. Multifocality and high recurrence rates are prominent clinical features of this disease and contribute to its high morbidity. Therefore, more sensitive and less invasive techniques could help identify individuals with asymptomatic disease. In this context, we used the micronucleus assay to evaluate whether cytogenetic alterations could be used as biomarkers for monitoring patients with a history of urothelial cell carcinoma (UCC). We determined the frequency of micronucleated urothelial cells (MNC) in exfoliated bladder cells from 105 patients with (n = 52) or without (n = 53) a history of UCC, all of whom tested negative for neoplasia by cytopathological and histopathological analyses. MNC frequencies were increased in patients with a history of UCC (non-smoker and smoker/ex-smoker patients vs non-smoker and smoker/ex-smoker controls; p<0.001), in non-smoker UCC patients (vs non-smoker controls; p<0.01), and in smoker/ex-smoker controls (vs non-smoker controls; p<0.001). Patients with a history of recurrent disease also demonstrated a higher MNC frequency compared to patients with non-recurrent neoplasia. However, logistic regression using smoking habits, age and gender as confounding factors did not confirm MNC frequency as a marker for UCC recurrence. Fluorescent in situ hybridization analysis (using a pan-centromeric probe) showed that micronuclei (MN) arose mainly from clastogenic events regardless of UCC and/or smoking histories. In conclusion, our results confirm previous indications that subjects with a history of UCC harbor genetically unstable cells in the bladder urothelium. Furthermore, these results support using the micronucleus assay as an important tool for monitoring patients with a history of UCC and tumor recurrence.

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Section: Discussionmentioning

confidence: 76%

Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma

et al. 2014

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“…Increased frequencies of chromosome aberration in patients suffering from different types of cancer [19,20], in families with high incidences of cancer [21] and in somatic cells of cancer patients after chemotherapy have been reported [2]. Based on the assumption that micronuclei are expressed in dividing cells that either contain chromosome fragments and/or whole chromosomes that are unable to migrate to the spindle poles during mitosis, we were able to use the MN assay to assess Different letters indicate statistically significant difference at p < 0.05 (a×b), and at p < 0.01 (a×c and b×c) chromosome damage in children with different malignant tumours prior to any treatment, as well as during chemotherapy.…”

Section: Discussionmentioning

confidence: 99%

DNA damage in lymphocytes and buccal mucosa cells of children with malignant tumours undergoing chemotherapy

et al. 2008

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The aim of the present study was to evaluate DNA damage (micronucleus) in cytokinesis-blocked lymphocytes and exfoliated buccal mucosa cells from children with malignant tumours and under chemotherapy. Micronucleated cells (MNCs) were assessed from children before and during chemotherapy. A total of 21 healthy children (controls), matched for gender and age, were used as control. The results pointed out higher frequencies of micronucleated lymphocytes in children with malignant tumour before any therapy when compared to healthy probands. Furthermore an increase of micronucleated lymphocytes during chemotherapy was detected when compared to the data obtained before chemotherapy. No statistically significant increases of MNCs were noticed in buccal mucosa cells at any of the timepoints evaluated. Taken together, these data indicate that the presence of malignant tumours may increase the frequency of DNA damage in circulating lymphocytes, these cells being more sensitive for detecting chromosome aberrations caused by anti-cancer drugs.

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“…The use of MN to assess chromosome damage in peripheral blood lymphocytes (PBL) has gained attention in the fields of environmental mutagenesis and prediction of adverse health effects. We have previously reported elevated MNC rates in PBL of cancer patients and carcinogens‐occupational exposed workers (Baciuchka‐Palmaro et al, 2002; Orsiere et al, 2006). MN result from lesions or adducts at the level of DNA or chromosome, or at the level of proteins (e.g., tubulin) involved in chromosome segregation.…”

Section: Discussionmentioning

confidence: 92%

Environmental nitration processes enhance the mutagenic potency of aromatic compounds

Bonnefoy

Chirón

Botta³

2010

Environmental Toxicology

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This work is an attempt to establish if aromatic nitration processes are always associated with an increase of genotoxicity. We determined the mutagenic and genotoxic effects of Benzene (B), Nitrobenzene (NB), Phenol (P), 2-Nitrophenol (2-NP), 2,4-Dinitrophenol (2,4-DNP), Pyrene (Py), 1-Nitropyrene (1-NPy), 1,3-Dinitropyrene (1,3-DNPy), 1,6-Dinitropyrene (1,6-DNPy), and 1,8-Dinitropyrene (1,8-DNPy). The mutagenic activities were evaluated with umuC test in presence and in absence of metabolic activation with S9 mix. Then, we used both cytokinesis-blocked micronucleus (CBMN) assay, in combination with fluorescent in situ hybridization (FISH) of human pan-centromeric DNA probes on human lymphocytes in order to evaluate the genotoxic effects. Analysis of all results shows that nitro polycyclic aromatic hydrocarbons (PAHs) are definitely environmental genotoxic/mutagenic hazards and confirms that environmental aromatic nitration reactions lead to an increase in genotoxicity and mutagenicity properties. Particularly 1-NPy and 1,8-DNPy can be considered as human potential carcinogens. They seem to be significant markers of the genotoxicity, mutagenicity, and potential carcinogenicity of complex PAHs mixtures present in traffic emission and industrial environment. In prevention of environmental carcinogenic risk 1-NPy and 1,8-DNPy must therefore be systematically analyzed in environmental complex mixtures in association with combined umuC test, CBMN assay, and FISH on cultured human lymphocytes. © 2010 Wiley Periodicals, Inc. Environ Toxicol, 2012.

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Acentromeric micronuclei are increased in peripheral blood lymphocytes of untreated cancer patients

Cited by 36 publications

References 48 publications

Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma

Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma

DNA damage in lymphocytes and buccal mucosa cells of children with malignant tumours undergoing chemotherapy

Environmental nitration processes enhance the mutagenic potency of aromatic compounds

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