AcERF1B and AcERF073 Positively Regulate Indole-3-acetic Acid Degradation by Activating <i>AcGH3.1</i> Transcription during Postharvest Kiwifruit Ripening

Gan, Zengyu; Shan, Nan; Wan, Chunpeng; Chen, Chuying; Zhu, Lu; Xu, Yan; Kai, Wenbin; Zhai, Xiaoyan; Chen, Jinyin

doi:10.1021/acs.jafc.1c03954

Cited by 12 publications

(3 citation statements)

References 63 publications

(156 reference statements)

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“…0.5 g of pulp from control and fucoidan-treated cucumber fruits was used for RNA extraction and gene expression analysis as previously described (Gan et al, 2021). The 2X M5 HiPer Realtime PCR Super mix kit (Mei5bio, Beijing, China) was used to perform quantitative real-time polymerase chain reaction (qRT-PCR) using gene-specific primers (Supplementary Table S1).…”

Section: Gene Expression Assaysmentioning

confidence: 99%

Fucoidan treatment alleviates chilling injury in cucumber by regulating ROS homeostasis and energy metabolism

et al. 2022

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IntroductionChilling injury is a major hindrance to cucumber fruit quality during cold storage.Methods and ResultsIn this study, we evaluated the effects of fucoidan on fruit quality, reactive oxygen species homeostasis, and energy metabolism in cucumbers during cold storage. The results showed that, compared with the control cucumber fruit, fucoidan-treated cucumber fruit exhibited a lower chilling injury index and less weight loss, as well as reduced electrolyte leakage and malondialdehyde content. The most pronounced effects were observed following treatment with fucoidan at 15 g/L, which resulted in increased 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radical scavenging rates and reduced superoxide anion production rate and hydrogen peroxide content. The expression and activity levels of peroxidase, catalase, and superoxide dismutase were enhanced by fucoidan treatment. Further, fucoidan treatment maintained high levels of ascorbic acid and glutathione, and high ratios of ascorbic acid/dehydroascorbate and glutathione/oxidized glutathione. Moreover, fucoidan treatment increased the activities of ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase and their gene expression. Fucoidan treatment significantly delayed the decrease in ATP and ADP, while preventing an increase in AMP content. Finally, fucoidan treatment delayed the decrease of energy charge and the activities and gene expression of H+-ATPase, Ca2+-ATPase, cytochrome c oxidase, and succinate dehydrogenase in cucumber fruits.ConclusionAltogether, our findings indicate that fucoidan can effectively enhance antioxidant capacity and maintain energy metabolism, thereby improving cucumber cold resistance during cold storage.

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Section: Gene Expression Assaysmentioning

confidence: 99%

Fucoidan treatment alleviates chilling injury in cucumber by regulating ROS homeostasis and energy metabolism

et al. 2022

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“…Statistical reliability of the internal branches was assessed using 1000 bootstrap replicates. Values at the nodes indicated the bootstrap percentages [31].…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Exogenous γ-Aminobutyric Acid (GABA) Treatment Suppresses Ethylene Biosynthesis in Hardy Kiwifruit (Actinidia aruguta) During Postharvest Storage

Dong¹,

Lan²,

Zhou³

et al. 2022

Preprint

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γ-Aminobutyric acid (GABA) plays an important role in plant development and postharvest properties of fruits. However, studies on regulation of kiwifruit ripening by GABA are scarce. Here, we evaluted physicochemical characters and the expression profile of genes related to ripening in hardy kiwifruit treated with exogenous GABA compared to control during postharvest storage period. The results showed that (1) exogenous GABA treatment not only improved the fruit quality but also inhibited respiration rate and ethylene production, as well as reduced the enzyme activities of ACC oxidase (ACO) and ACC synthase (ACS), via down-regulating the expression of AaACO1 and AaACO3, AaACS1 and AaACS2 during kiwifruit storage period; (2) on the one hand, overexpression of AaGAD1 or AaGAD4 gene which is involved in GABA biosynthesis inhibited ethylene production by reducing the enzyme activities of ACO and ACS, whereas silencing of AaGAD1 or AaGAD4 gene led to the reverse effect. On the other hand, overexpression of AaGAD1 or AaGAD4 decreased the expression levels of AaACO1 and AaACO3, AaACS1 and AaACS2, whereas silencing of AaGAD1 or AaGAD4 gene increased the expression levels of these four genes. Above all, our findings demonstrate that exogenous GABA treatment could improve the storage quality and extend the shelf life of kiwifruit, Additionally, the content of GABA was participated in the regulation of ethylene biosynthesis at the molecular level.

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“…It was reported that IAA content accumulated during fruit ripening but decreased rapidly during fruit senescence ( Trainotti et al., 2007 ; Chen et al., 2014 ). Exogenous treatment with IAA could delay the ripening process in harvested kiwifruit ( Gan et al., 2021 ). The protective effects of melatonin as an antioxidant has been frequently investigated ( Reiter et al., 2001 ; Iriti et al., 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Hydrogen-rich water treatment increased several phytohormones and prolonged the shelf life in postharvest okras

Dong

Cao²,

Zhou³

et al. 2023

Front. Plant Sci.

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Hydrogen-rich water (HRW) treatment has been reported to delay the softening and senescence of postharvest okras, but its regulatory mechanism remains unclear. In this paper, we investigated the effects of HRW treatment on the metabolism of several phytohormones in postharvest okras, which act as regulatory molecules in fruit ripening and senescence processes. The results showed that HRW treatment delayed okra senescence and maintained fruit quality during storage. The treatment upregulated all of the melatonin biosynthetic genes such as AeTDC, AeSNAT, AeCOMT and AeT5H, contributing to the higher melatonin content in the treated okras. Meanwhile, increased transcripts of anabolic genes but lower expression of catabolic genes involved in indoleacetic acid (IAA) and gibberellin (GA) metabolism were observed in okras when treated with HRW, which was related to the enhanced levels of IAA and GA. However, the treated okras experienced lower abscisic acid (ABA) content as compared to the non-treated fruit due to the down-regulation of its biosynthetic genes and up-regulation of the degradative gene AeCYP707A. Additionally, there was no difference in γ-aminobutyric acid between the non-treated and HRW-treated okras. Collectively, our results indicated that HRW treatment increased levels of melatonin, GA and IAA, but decreased ABA content, which ultimately delayed fruit senescence and prolonged shelf life in postharvest okras.

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AcERF1B and AcERF073 Positively Regulate Indole-3-acetic Acid Degradation by Activating AcGH3.1 Transcription during Postharvest Kiwifruit Ripening

Cited by 12 publications

References 63 publications

Fucoidan treatment alleviates chilling injury in cucumber by regulating ROS homeostasis and energy metabolism

Fucoidan treatment alleviates chilling injury in cucumber by regulating ROS homeostasis and energy metabolism

Exogenous γ-Aminobutyric Acid (GABA) Treatment Suppresses Ethylene Biosynthesis in Hardy Kiwifruit (Actinidia aruguta) During Postharvest Storage

Hydrogen-rich water treatment increased several phytohormones and prolonged the shelf life in postharvest okras

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