Acetaldehyde formation during deproteinization of human blood samples containing ethanol

Stowell, Allan; Greenway, Robert M.; Batt, Richard D.

doi:10.1016/0006-2944(77)90075-8

Cited by 53 publications

(9 citation statements)

References 12 publications

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“…A rapid disappearance artifact (21) and elevation of acetaldehyde levels with hemolysis in vitro (22) have been described. Recently, it has been reported that the immediate precipitation of blood proteins helps to minimize these changes (23). The present studies may provide an explanation for these findings.…”

Section: Resultssupporting

confidence: 68%

Acetaldehyde adducts with hemoglobin.

Stevens¹,

Fantl²,

Newman³

et al. 1981

J. Clin. Invest.

310

104

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Section: Resultssupporting

confidence: 68%

Acetaldehyde adducts with hemoglobin.

Stevens¹,

Fantl²,

Newman³

et al. 1981

J. Clin. Invest.

310

104

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“…The significant within-subject correlation in the artifactual formation of acetaldehyde between separate experimental sessions observed in the present study is intriguing and suggests an underlying subjectrelated regulatory factor. Given that the artifactual formation decreases with increasing blood dilution (Eriksson et al, 1982;Stowell et al, 1977) and that most of the artifactual formation occurs in the presence of erythrocytes (Fukunaga et al, 1993a;Stowell et al, 1977), it seems that variations in the hemoglobin level (or in any other eryth- rocyte component) might, at least in part, affect the magnitude of the artifactual acetaldehyde formation. The issue should be addressed in future studies.…”

Section: Discussionmentioning

confidence: 97%

Ethanol, Acetaldehyde, Acetate, and Lactate Levels After Alcohol Intake in White Men and Women: Effect of 4‐Methylpyrazole

Sarkola

Iles

Kohlenberg-Mueller

et al. 2002

Alcoholism Clin & Exp Res

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The alcohol-induced elevation in blood acetate level is caused, in part, by ADH-mediated ethanol oxidation. Although no evidence was found for measurable acetaldehyde levels in the peripheral venous blood during alcohol intoxication, the effect of 4-MP on breath acetaldehyde in women supports the view that ADH-mediated acetaldehyde elevations reflected in the airways, but too low to be detected in the peripheral venous blood, may occur in women during alcohol intoxication in the present experimental conditions.

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“…Acetaldehyde was analysed using headspace gas chromatography as described previously 22. To control for non-enzymatic artefactual acetaldehyde formation from ethanol during the protein precipitation,23 PCA was added simultaneously with ethanol into additional incubation vials (incubation time 0) and the revealed values were subtracted from acetaldehyde values obtained after the 60 minute incubation period. Acetaldehyde production was related to the protein concentration of the supernatant determined by the method of Lowry et al 24…”

Section: Methodsmentioning

confidence: 99%

Ciprofloxacin decreases the rate of ethanol elimination in humans

Tillonen

Homann

Rautio

et al. 1999

Gut

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Background-Extrahepatic ethanol metabolism is postulated to take place via microbial oxidation in the colon, mediated by aerobic and facultative anaerobic bacteria. Aims-To evaluate the role of microbial ethanol oxidation in the total elimination rate of ethanol in humans by reducing gut flora with ciprofloxacin. Methods-Ethanol was administered intravenously at the beginning and end of a one week period to eight male volunteers. Between ethanol doses volunteers received 750 mg ciprofloxacin twice daily. Results-A highly significant (p=0.001) reduction in the ethanol elimination rate (EER) was detected after ciprofloxacin medication. Mean (SEM) EER was 107.0 (5.3) and 96.9 (4.8) mg/kg/h before and after ciprofloxacin, respectively. Faecal Enterobacteriaceae and Enterococcus sp. were totally absent after medication, and faecal acetaldehyde production capacity was significantly (p<0.05) decreased from 0.91 (0.15) to 0.39 (0.08) nmol/min/mg protein. Mean faecal alcohol dehydrogenase (ADH) activity was significantly (p<0.05) decreased after medication, but ciprofloxacin did not inhibit human hepatic ADH activity in vitro. Conclusions-Ciprofloxacin treatment decreased the ethanol elimination rate by 9.4%, with a concomitant decrease in intestinal aerobic and facultative anaerobic bacteria, faecal ADH activity, and acetaldehyde production. As ciprofloxacin has no eVect on liver blood flow, hepatic ADH activity, or cytochrome CYP2E1 activity, these eVects are probably caused by the reduction in intestinal flora. (Gut 1999;44:347-352)

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Acetaldehyde formation during deproteinization of human blood samples containing ethanol

Cited by 53 publications

References 12 publications

Acetaldehyde adducts with hemoglobin.

Acetaldehyde adducts with hemoglobin.

Ethanol, Acetaldehyde, Acetate, and Lactate Levels After Alcohol Intake in White Men and Women: Effect of 4‐Methylpyrazole

Ciprofloxacin decreases the rate of ethanol elimination in humans

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