Acetate Enhances the Specific Consumption Rate of Toluene Under Denitrifying Conditions

Martínez-Hernández, Sergio; Olguín, Eugenia J.; Gómez, Jorge; Cuervo-López, Flor de María

doi:10.1007/s00244-009-9321-z

Cited by 20 publications

(27 citation statements)

References 27 publications

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“…The temperatures of the column, injector and detector were 50, 100 and 100 • C, respectively, with helium as the carrier gas at 16 mL min −1 . Acetate concentration was determined by gas chromatography (Hewlett Packard 5890, USA) as previously described by Martínez-Hernández et al [19]. Organic and inorganic carbon was determined using a total organic carbon (TOC) analyser (Shimadzu, TOC-VCSN, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…Sanford and Tiedje [23] proposed that, under denitrifying conditions, 2-CP consumption is initiated by the reduction of 2-CP to phenol and requires the addition of an electron donor such as acetate to initiate the dehalogenation process. It has been also suggested that the addition of readily oxidable carbon sources might improve the consumption of recalcitrant aromatic compounds [10,19]. To obtain a better understanding about how 2-CP consumption could be improved under denitrifying conditions, phenol and acetate were tested as co-substrates.…”

Section: -Chlorophenol Consumption At Stoichiometric C/n Ratiomentioning

confidence: 99%

“…These included cell adaptation to the pollutant and the addition of alternative carbon sources (co-substrate) [17]. It has been suggested that the addition of readily oxidable carbon sources might be acting as inducing agents of oxidizing enzymes or by providing ISSN [10,18,19]. Moreover, the addition of an external electron source can be necessary for chlorophenol consumption when the pathway initiates with a reductive dechlorination [20][21][22].…”

Section: Introductionmentioning

confidence: 98%

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Effect of phenol and acetate addition on 2-chlorophenol consumption by a denitrifying sludge

Martínez‐Gutiérrez¹,

González‐Márquez²,

Martínez-Hernández³

et al. 2012

Environmental Technology

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Chlorophenols are widely distributed in the environment. Various strategies have been used to improve their biological elimination under anaerobic conditions; however, such information is still scarce. The aim of this study was to evaluate in batch assays the consumption of 2-chlorophenol (2-CP) by a denitrifying sludge and the influence of acetate or phenol as co-substrates in the 2-CP consumption. It was observed that phenol (69 and 92 mg phenol-C L(-1)) and acetate (60 and 108 mg acetate-C L(-1)) enhanced 2-CP consumption by the denitrifying sludge, increasing both the efficiency (up to 100%) and specific rate of 2-CP consumption. When phenol was added at 92 mg C L(-1), the specific consumption rate of 2-CP increased 2.6 times with respect to the control lacking co-substrates, whereas with acetate (108 mgC L(-1)) the increase was 9.0 times. Acetate appeared to be a better co-substrate for 2-CP consumption, obtaining a specific consumption rate of 2.48 +/- 0.14 mg 2-CP-C g(-1) VSS d(-1) at 108 mg acetate-C L(-1). The mass balance analysis indicated that the denitrifying sludge was able to simultaneously mineralize 2-CP, phenol or acetate (E2-CP, E(Phenol), and E(Acetate) close to 100% [E = consumption efficiency], Y(HCO3-) of 0.90 +/- 0.10 [Y = yield]) and reduce nitrate to nitrogen gas (E(NO3-) of 100% and Y(N2) of 0.96 +/- 0.02). It was shown that the addition of co-substrates as phenol or acetate could be a good alternative for improving the elimination of chlorophenols from wastewaters by denitrifying sludges.

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Section: Methodsmentioning

confidence: 99%

Section: -Chlorophenol Consumption At Stoichiometric C/n Ratiomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Effect of phenol and acetate addition on 2-chlorophenol consumption by a denitrifying sludge

Martínez‐Gutiérrez¹,

González‐Márquez²,

Martínez-Hernández³

et al. 2012

Environmental Technology

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“…Figure 3(b) shows the influent and effluent TOC change in UASB with a high carbon concentration and a high nitrogen loading rate. Sodium succinate [12,28] was the only carbon source in the denitrification process, and the detected gas did not contain carbon dioxide, which might be produced in the reactor. The average TOC difference between influent and effluent was 72.02 mg L ¡1 , which can be used by denitrification organisms under anoxic conditions in UASB.…”

Section: Cod Removal Rate and Toc Change During The Set-up Processmentioning

confidence: 99%

Study of the dynamics and material transformation characteristics of nitrite denitrification in UASB

Zhao

Guo

Lian

et al. 2015

Biotechnology & Biotechnological Equipment

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Nitrite is known to accumulate in wastewater treatment plants and play an important role during the biological denitrogenation process. In this study, wastewater with high nitrite concentration was effectively treated, and the possible material transformation of nitrite and carbon by microorganisms is discussed herein. While inoculating pre-acclimatized floccular sludge, nitrite-denitrifying granular sludge was obtained after approximately 15 days of cultivation in a denitrifying upflow anaerobic sludge blanket (UASB) reactor. The nitrite removal efficiency (R NO2ÀN (%)) was always greater than 98%. At the same time, the nitrite removal rate (R NO2ÀN ) reached 0.46 g N g VSS ¡1 d ¡1 . The granular sludge taken from the reactor was characterized. The fluorescence in situ hybridization (FISH) result showed that the microbial community structure was also changed during the granular sludge formation process and that the nitrite denitrifying bacteria increased in population size. Based on the carbon balance, the stoichiometric equation of nitrite denitrification was obtained and a biomass yield value was calculated. The practical ratio of chemical oxygen demand (COD) and nitrite was 2:1, which was higher than the theoretical value. The measured effluent pH was negligibly different from the stoichiometric value.

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“…The glucose concentration was determined using a YSI 2700 biochemical analyzer (YSI-2700 Select, USA) with a YSI membrane 2365. Acetate concentration was quantified by gas chromatography (Hewlett-Packard 5890, USA) using a flame ionization detector and a steel packed column (Porapak Q Mesh 80-100, USA) [28]. Soluble organic and inorganic carbon was measured in a total organic carbon (TOC) analyzer (Shimadzu, TOC-VCSN, Japan).…”

Section: Batch Assaysmentioning

confidence: 99%

Consumption of 2-Chlorophenol Using Anaerobic Sludge: Physiological and Kinetic Analysis

Martínez‐Gutiérrez

Texier

Cuervo-López

et al. 2014

Appl Biochem Biotechnol

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Chlorophenols are toxic and recalcitrant compounds produced by many industrial. Different strategies have been used to improve their biological consumption, but there is insufficient information to understand how the process is carried out. The objective of this study was to evaluate in batch tests the effect of the addition of phenol, acetate, or glucose as electron donors at different concentrations on the efficiencies, yields, and specific rates of 2-chlorophenol (2-CP) consumption. The addition of phenol (177.6 mg C/L), acetate (127.6 mg C/L), or glucose (77.6 mg C/L) increased the 2-CP consumption efficiency up to 54.6, 98.6, and 97.8 %, respectively. With respect to the control assay without electron donor, the specific rate of 2-CP consumption was up to 2.5 times higher with phenol (177.6 mg C/L), 8.4 times higher with acetate (127.6 mg C/L), and 3 times higher with glucose (127.6 mg C/L). The results showed that the type and concentration of electron donor determine the physiological behavior of the anaerobic sludge, modifying efficiency, yield, and specific rate values of the 2-CP consumption process. The addition of readily oxidable cosubstrates seems to be a good alternative and might be used for the biological treatment of industrial wastewater polluted with chlorinated phenols.

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Acetate Enhances the Specific Consumption Rate of Toluene Under Denitrifying Conditions

Cited by 20 publications

References 27 publications

Effect of phenol and acetate addition on 2-chlorophenol consumption by a denitrifying sludge

Effect of phenol and acetate addition on 2-chlorophenol consumption by a denitrifying sludge

Study of the dynamics and material transformation characteristics of nitrite denitrification in UASB

Consumption of 2-Chlorophenol Using Anaerobic Sludge: Physiological and Kinetic Analysis

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