Acetate metabolism and its regulation in Corynebacterium glutamicum

Gerstmeir, Robert; Wendisch, Volker F.; Schnicke, Stephanie; Ruan, Hong; Farwick, Mike; Reinscheid, Dieter J.; Eikmanns, Bernhard J.

doi:10.1016/s0168-1656(03)00167-6

Cited by 186 publications

(156 citation statements)

References 98 publications

Supporting

Mentioning

144

Contrasting

Order By: Relevance

“…Wendisch et al [26] showed a two-to fourfold higher carbon flux into tricarboxylic acid cycle when C. glutamicum cells were grown in the presence of acetate. Their further studies showed that the expression of genes of glyoxylate cycle (aceA and aceB) was upregulated in the presence of acetate [27]. Yu et al [28] reported that glyoxylate cycle is required for the overproduction of glutamate and the glutamate production was insignificantly inhibited after aceA gene deletion.…”

Section: Effect Of Tricarboxylic Acid Cycle Intermediates On L-ornithmentioning

confidence: 99%

Optimization of Fermentation Conditions of the Engineered Corynebacterium Glutamicum to Enhance L-Ornithine Production by Response Surface Methodology

Lu¹,

Jiang²,

Chen³

et al. 2011

J Biotechnol Biomaterial

View full text Add to dashboard Cite

Section: Effect Of Tricarboxylic Acid Cycle Intermediates On L-ornithmentioning

confidence: 99%

Optimization of Fermentation Conditions of the Engineered Corynebacterium Glutamicum to Enhance L-Ornithine Production by Response Surface Methodology

Lu¹,

Jiang²,

Chen³

et al. 2011

J Biotechnol Biomaterial

View full text Add to dashboard Cite

“…, as recalculated from data published by Gerstmeir et al (2003). In biotin excess, the specific rate of glucose consumption was significantly higher than that in biotin limitation.…”

mentioning

confidence: 94%

Regulation of ldh expression during biotin-limited growth of Corynebacterium glutamicum

et al. 2009

View full text Add to dashboard Cite

Corynebacterium glutamicum is a biotin-auxotrophic bacterium and some strains efficiently produce glutamic acid under biotin-limiting conditions. In an effort to understand C. glutamicum metabolism under biotin limitation, growth of the type strain ATCC 13032 was investigated in batch cultures and a time-course analysis was performed. A transient excretion of organic acids was observed and we focused our attention on lactate synthesis. Lactate synthesis was due to the ldh-encoded l-lactate dehydrogenase (Ldh). Features of Ldh activity and ldh transcription were analysed. The ldh gene was shown to be regulated at the transcriptional level by SugR, a pleiotropic transcriptional repressor also acting on most phosphotransferase system (PTS) genes. Electrophoretic mobility shift assays (EMSAs) and site-directed mutagenesis allowed the identification of the SugR-binding site. Effector studies using EMSAs and analysis of ldh expression in a ptsF mutant revealed fructose 1-phosphate as a highly efficient negative effector of SugR. Fructose 1,6-bisphosphate also affected SugR binding.

show abstract

“…The transcriptional regulator GlxR contains a domain with similarity to the cAMP-binding motifs of Crp from E. coli and, in fact, cAMP is essential for binding of GlxR to the aceA/aceB intergenic region in C. glutamicum (Kim et al, 2004;Letek et al, 2006). The regulation of C. glutamicum metabolism in the presence of various carbon sources clearly differs from that of E. coli or B. subtilis (Gerstmeir et al, 2003;Hayashi et al, 2002;Letek et al, 2006;Muffler et al, 2002). No direct evidence has been found of a CCR system in C. glutamicum (Bruckner & Titgemeyer, 2002;Gerstmeir et al, 2003;Han et al, 2007), although a CCR-like phenomenon has been reported from cells grown on medium containing glucose with either glutamate (Gerstmeir et al, 2003;Kramer et al, 1990;Kronemeyer et al, 1995) or ethanol (Arndt & Eikmanns, 2007;Kotrbova-Kozak et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…2O). However, these accumulated carbon sources may not affect the transcripts of genes strongly when glucose remains abundant (Gerstmeir et al, 2003). Interestingly, glucose induced high levels of expression of TCA cycle genes during the early stationary phase, whereas the transcript levels were highest at the mid-exponential phase in acetate medium (Figs 1 and 2).…”

mentioning

confidence: 97%

Effect of carbon source availability and growth phase on expression of Corynebacterium glutamicum genes involved in the tricarboxylic acid cycle and glyoxylate bypass

2008

View full text Add to dashboard Cite

The effect of different carbon sources on the expression of tricarboxylic acid (TCA) cycle genes, along with glyoxylate bypass genes, in Corynebacterium glutamicum was determined. All TCA cycle genes were coordinately expressed in medium containing acetate. Growth in the presence of acetate gave rise to abundant expression of most TCA cycle genes, with the level of gltA transcript being the highest. However, when the cells entered the stationary phase triggered by acetate exhaustion, all genes were repressed, except sucCD and mdhB, which were slightly induced. Acetate withdrawal from the growth medium during the exponential phase also led to rapid repression of most TCA cycle genes and a corresponding twofold increase in the expression of sucCD, which were strongly induced by citrate and succinate. In addition, glucose depletion during the stationary phase led to a corresponding 8-20-fold induction of the sucCD, aceA and aceB genes. Addition of glucose to acetate medium resulted in about 10-fold induction of sucCD. The strong dependence of TCA cycle sucCD and glyoxylate bypass aceA and aceB expression on carbon source availability was confirmed and the regulatory system will be studied precisely. INTRODUCTIONCorynebacterium glutamicum, a non-pathogenic, facultative anaerobic Gram-positive soil bacterium, is widely used in the industrial production of numerous metabolites, including amino acids and organic acids (Kinoshita et al., 1957;Liebl, 2005;Nishimura et al., 2007). In contrast to closely related, medically important pathogenic species, such as Corynebacterium diphtheriae and Mycobacterium tuberculosis, C. glutamicum is generally recognized as a nonhazardous organism (Dover et al., 2004;Funke et al., 1997). Furthermore, this organism has gained increasing interest as a suitable model organism for high-G+C-content Grampositive bacteria in general and for Corynebacterineae, a suborder of the Actinomycetes, in particular.One of the central metabolic pathways in C. glutamicum and in other aerobic bacteria is the tricarboxylic acid (TCA) cycle, which is responsible for the complete oxidation of acetyl-CoA derived from various substrates and for the provision of precursors for amino acid biosynthesis. TCA cycle intermediates are commonly used by other metabolic reactions in a wide variety of cell types. Due to the commercial importance of the amino acids and organic acids produced by C. glutamicum, the control of TCA cycle enzyme activities has been the subject of intensive studies, and the phenotypes of mutants affecting TCA cycle genes have been analysed (Eikmanns et al., 1994(Eikmanns et al., , 1995Molenaar et al., 1998Molenaar et al., , 2000Usuda et al., 1996;Wittmann & De Graaf, 2005). However, limited work has been devoted to the regulation of the expression of C. glutamicum TCA cycle genes in response to different growth phases and carbon sources. Recently we have reported the transcription of C. glutamicum genes involved in the TCA cycle and glyoxylate bypass (Han et al., 2008). In fact, aspects of the re...

show abstract

Acetate metabolism and its regulation in Corynebacterium glutamicum

Cited by 186 publications

References 98 publications

Optimization of Fermentation Conditions of the Engineered Corynebacterium Glutamicum to Enhance L-Ornithine Production by Response Surface Methodology

Optimization of Fermentation Conditions of the Engineered Corynebacterium Glutamicum to Enhance L-Ornithine Production by Response Surface Methodology

Regulation of ldh expression during biotin-limited growth of Corynebacterium glutamicum

Effect of carbon source availability and growth phase on expression of Corynebacterium glutamicum genes involved in the tricarboxylic acid cycle and glyoxylate bypass

Contact Info

Product

Resources

About