Acetoacetyl‐CoA synthetase; a lipogenic enzyme in rat tissues

Buckley, Brendan M.; Williamson, Dermot H.

doi:10.1016/0014-5793(75)80406-6

Cited by 47 publications

(27 citation statements)

References 19 publications

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“…This finding is in accordance with the reported low activity level of acetoacetyl-CoA synthetase in rat liver (37,38) and the ability of brain cytosolic enzyme systems to utilize acetoacetate during the suckling period (38). Utilization of ketone bodies for the Krebs cycle and respiration was also reported to have a specific developmental profile with the maximum at the suckling-myelination period (31).…”

Section: C20 Des C10supporting

confidence: 90%

Utilization of carbon from dietary polyunsaturates for brain cholesterol synthesis during early postnatal development in the rat: a ¹³c nmr study

Likhodii

Cunnane

1995

Magnetic Resonance in Med

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Incorporation of 13C from a dietary precursor into cholesterol was studied in neonatal rats. Rats were given uniformly 13C-enriched polyunsaturated fatty acids intragastrically and total lipid extracts of liver and brain were analyzed by 13C-NMR 1, 4, 8, and 15 days later. 13C-enrichment was detected in brain but not in liver cholesterol. Maximal 13C-labeling was observed 4 days after injection of the label. Spectra revealed that 70% of newly incorporated 13C had 13C as an adjacent neighbor, the other 30% had 12C as the neighbor. Double quantum NMR revealed the arrangement in the cholesterol skeleton of the 13C-13C pairs transferred from precursors to cholesterol. Desmosterol, an intermediate of cholesterol synthesis, was identified in the spectra of brain lipids. Comparison of 13C-13C unit arrangements in both cholesterol and desmosterol allowed carbons 26 and 27 of desmosterol to be unambiguously assigned.

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Section: C20 Des C10supporting

confidence: 90%

Utilization of carbon from dietary polyunsaturates for brain cholesterol synthesis during early postnatal development in the rat: a ¹³c nmr study

Likhodii

Cunnane

1995

Magnetic Resonance in Med

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“…In mammals, AA‐CoA synthetase is present in the cytosolic fractions of various tissues [4–7], especially lipogenic ones, and acetoacetate is known to be effectively incorporated into cholesterol and fatty acids [8]. These results indicate that AA‐CoA synthetase may supply acetyl units to cytosolic compartment for cholesterol and/or fatty acid biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

cDNA‐derived amino acid sequence of acetoacetyl‐CoA synthetase from rat liver¹

Iwahori¹,

Takahashi²,

Nakamoto³

et al. 2000

FEBS Letters

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In order to examine the primary structure of acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, EC 6.2.1.16; AA-CoA synthetase), the cDNA clone encoding this enzyme has been isolated from the cDNA library which was prepared from the liver of rat fed a diet supplemented with 4% cholestyramine and 0.4% pravastatin for 4 days. Nucleotide sequence analysis of cloned cDNA revealed that AA-CoA synthetase of rat liver contains an open reading frame of 2019 nucleotides, and the deduced amino acid sequence (672 amino acid residues) bears 25.0 and 38.9% homologies with acetyl-CoA synthetases of Saccharomyces cerevisiae and Archaeoglobus fulgidus, respectively.

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“…Both tissues are remarkably adept in being able to utilize substrates in whatever form they are presented. For example in mammary gland, MCFA can be synthe sized from either glucose or ketone bodies [18], but in creasing dietary lipid replaces synthesis de novo [15,16]. Likewise, in the brain of the suckling rat glucose replaces ketone bodies as the main precursor of cholesterol when circulating ketone body concentrations (and MCFA) are low [53].…”

Section: Brain H Eterogeneity and Su B Stra Te Utilizationmentioning

confidence: 99%

“…Ketone bodies, and in particular acctoacetatc, can act as lipogenic precursors in lactating mammary gland [18], but they also inhibit the conversion of glucose to lipid [19], Thus ketone bodies (produced by the liver) could be an external signal that informs the mammary gland of the nutritional state of the mother, because their concen tration in the blood increases in starvation and on feed ing a high-fat diet. There is no evidence at present that ketone bodies inhibit the uptake of pre-formed lipid by the gland.…”

Section: Om Po Sitio N Of M Ilk Lipidmentioning

confidence: 99%

Strategies for the Supply of Lipid Substrates during Post-Natal Brain Development: A Tale of Two Tissues

Williamson

Lund

1993

Dev Neurosci

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In the suckling neonate the source of lipid and other substrates for brain development is the mother''s milk. The lactating mammary gland is a highly versatile organ which is able to utilize a range of substrates for milk lipid production including glucose and ketone bodies (for de novo synthesis), triacylglycerols and non-esterified fatty acids. The composition of the milk lipid alters with changes in the substrates available to the mammary gland. The neonate has to process the milk triacylglycerols to forms (ketone bodies, medium-chain fatty acids) more suitable for extraction by the developing brain. The strategies employed to allow this flexibility in the lactating rat and its offspring are discussed.

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Acetoacetyl‐CoA synthetase; a lipogenic enzyme in rat tissues

Cited by 47 publications

References 19 publications

Utilization of carbon from dietary polyunsaturates for brain cholesterol synthesis during early postnatal development in the rat: a ¹³c nmr study

Utilization of carbon from dietary polyunsaturates for brain cholesterol synthesis during early postnatal development in the rat: a ¹³c nmr study

cDNA‐derived amino acid sequence of acetoacetyl‐CoA synthetase from rat liver¹

Strategies for the Supply of Lipid Substrates during Post-Natal Brain Development: A Tale of Two Tissues

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Acetoacetyl‐CoA synthetase; a lipogenic enzyme in rat tissues

Cited by 47 publications

References 19 publications

Utilization of carbon from dietary polyunsaturates for brain cholesterol synthesis during early postnatal development in the rat: a 13c nmr study

Utilization of carbon from dietary polyunsaturates for brain cholesterol synthesis during early postnatal development in the rat: a 13c nmr study

cDNA‐derived amino acid sequence of acetoacetyl‐CoA synthetase from rat liver1

Strategies for the Supply of Lipid Substrates during Post-Natal Brain Development: A Tale of Two Tissues

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Utilization of carbon from dietary polyunsaturates for brain cholesterol synthesis during early postnatal development in the rat: a ¹³c nmr study

Utilization of carbon from dietary polyunsaturates for brain cholesterol synthesis during early postnatal development in the rat: a ¹³c nmr study

cDNA‐derived amino acid sequence of acetoacetyl‐CoA synthetase from rat liver¹