Acetone

Kalapos, Miklös Péter; Ruzsányi, Veronika

doi:10.1016/b978-0-12-824315-2.00393-6

Cited by 2 publications

(2 citation statements)

References 105 publications

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“…The standard dogma has persisted for so long that acetone is the end product of metabolism in animals and humans. Acetone evaporates easily, even from water and soil (Kalapos, 2014). The addition of acetate and glucose to the bacterial growth substrate can increase the production of acetone (Gao et al, 2015).…”

Section: Selection Of Primers For the Volatile Synthesis-encoding Genementioning

confidence: 99%

Primer Design of Volatile Synthesis Coding Genes in Ralstonia syzygii subsp. celebesensis

Damanik,

Prakoso,

Triyana

et al. 2023

JPTI

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Microbes produce various types of volatile organic compounds (VOCs) through metabolism, which can be used for diagnostic purposes. Microbes' types and classes of VOCs are very wide, including fatty acid derivatives (hydrocarbons, alcohols, and ketones), aromatic compounds, nitrogen-containing compounds, and volatile sulfur compounds. Microbial volatile organic compounds (VOCs) can also be divided into several chemical classes: alkenes, alcohols, ketones, benzos, pyrazines, sulfides, acids, esters, and terpenes. This study aimed to design primers for genes encoding volatile synthesis in Ralstonia syzygii subsp. celebesensis, which causes blood disease in the banana plant. Some of the genes involved are adc (acetone synthesis), adhP (ethanol synthesis), ilvA, nirBD (ammonia synthesis), mdcA (propionic acid synthesis), cysI (hydrogen sulfide synthesis), and speBC (putrescine synthesis). Primers were designed and examined for specificity in silico using Primer3Plus, Geneious Prime, and BLAST programs. The numbers of nine pairs designed primers were successfully amplifying the related nine VOC genes of R. syzygii subsp. celebesensis for qPCR.

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Section: Selection Of Primers For the Volatile Synthesis-encoding Genementioning

confidence: 99%

Primer Design of Volatile Synthesis Coding Genes in Ralstonia syzygii subsp. celebesensis

Damanik,

Prakoso,

Triyana

et al. 2023

JPTI

View full text Add to dashboard Cite

show abstract

“…Some of the VOCs that may be present in the breath are oxygen-containing compounds (acetone, methanol, ethanol, etc.) [ 42 – 45 ], hydrocarbons (ethane, pentane, isoprene, etc.) [ 46 – 48 ], sulfur-containing compounds (ethyl mercaptane, dimethyl sulfide, dimethyl disulfide, etc.)…”

Section: Introductionmentioning

confidence: 99%

Smelling the Disease: Diagnostic Potential of Breath Analysis

2023

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Breath analysis is a relatively recent field of research with much promise in scientific and clinical studies. Breath contains endogenously produced volatile organic components (VOCs) resulting from metabolites of ingested precursors, gut and air-passage bacteria, environmental contacts, etc. Numerous recent studies have suggested changes in breath composition during the course of many diseases, and breath analysis may lead to the diagnosis of such diseases. Therefore, it is important to identify the disease-specific variations in the concentration of breath to diagnose the diseases. In this review, we explore methods that are used to detect VOCs in laboratory settings, VOC constituents in exhaled air and other body fluids (e.g., sweat, saliva, skin, urine, blood, fecal matter, vaginal secretions, etc.), VOC identification in various diseases, and recently developed electronic (E)-nose-based sensors to detect VOCs. Identifying such VOCs and applying them as disease-specific biomarkers to obtain accurate, reproducible, and fast disease diagnosis could serve as an alternative to traditional invasive diagnosis methods. However, the success of VOC-based identification of diseases is limited to laboratory settings. Large-scale clinical data are warranted for establishing the robustness of disease diagnosis. Also, to identify specific VOCs associated with illness states, extensive clinical trials must be performed using both analytical instruments and electronic noses equipped with stable and precise sensors.

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Acetone

Cited by 2 publications

References 105 publications

Primer Design of Volatile Synthesis Coding Genes in Ralstonia syzygii subsp. celebesensis

Primer Design of Volatile Synthesis Coding Genes in Ralstonia syzygii subsp. celebesensis

Smelling the Disease: Diagnostic Potential of Breath Analysis

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