Acetone-butanol fermentation revisited

Jones, David T.; Woods, David R.

doi:10.1128/mr.50.4.484-524.1986

Cited by 983 publications

(261 citation statements)

References 250 publications

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“…In all cases, the strains did not show obvious growth at pH 4.0. This behavior is consistent with previous report that some solvent producing Clostridium strain cannot produce solvent at initial pH lower than 4.5 [10]. Because acetic acid and butyric acid have pKa of 4.76 and 4.82, respectively, fermentation carried out at pH significantly below the pKa of these acids can result in accumulation of un-dissociated acids and premature cessation of ABE production.…”

supporting

confidence: 92%

Exploring Co-fermentation of Glucose and Galactose using Clostridium acetobutylicum and Clostridium beijerinckii for Biofuels

Liu

Zhuo

et al. 2017

Natural Product Communications

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There is growing interest to produce fuels from algae, namely the third generation biofuel. Galactose and glucose are basic chemicals for many red macroalgae, but fermentation of the mixed sugars may suffer significant glucose repression using yeast. Therefore, another fermentation, acetone-butanol-ethanol (ABE) fermentation of the mixed sugars was studied using Clostridium acetobutylicum and Clostridium beijerinckii. Both strains can use either galactose or glucose, and showed an optimal pH at ~ 5.0. Co-fermentation of the mixed sugar showed simultaneous consumption of glucose and galactose, and exhibited solvent production of 4.19 and 4.57 g/L using Clostridium acetobutylicum and Clostridium beijerinckii, respectively. The fermentation can become more industrially practical if improvement in galactose consumption can be further improved in the future.

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supporting

confidence: 92%

Exploring Co-fermentation of Glucose and Galactose using Clostridium acetobutylicum and Clostridium beijerinckii for Biofuels

Liu

Zhuo

et al. 2017

Natural Product Communications

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“…Accordingly, microbial isobutanol production from glucose has been established in several organisms [3, 4] such as Escherichia coli [5, 6], Bacillus subtilis [7], and Corynebacterium glutamicum [8, 9] under aerobic or anaerobic conditions. Nevertheless, the toxicity of isobutanol to these organisms is a major drawback preventing their use for industrial scale production with even further improved key performance values [4, 10].…”

Section: Introductionmentioning

confidence: 99%

Micro‐aerobic production of isobutanol with engineered Pseudomonas putida

Ankenbauer

Nitschel

Teleki

et al. 2021

Engineering in Life Sciences

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Pseudomonas putida KT2440 is emerging as a promising microbial host for biotechnological industry due to its broad range of substrate affinity and resilience to physicochemical stresses. Its natural tolerance towards aromatics and solvents qualifies this versatile microbe as promising candidate to produce next generation biofuels such as isobutanol. In this study, we scaled‐up the production of isobutanol with P. putida from shake flask to fed‐batch cultivation in a 30 L bioreactor. The design of a two‐stage bioprocess with separated growth and production resulted in 3.35 gisobutanol L–1. Flux analysis revealed that the NADPH expensive formation of isobutanol exceeded the cellular catabolic supply of NADPH finally causing growth retardation. Concomitantly, the cell counteracted to the redox imbalance by increased formation of 2‐ketogluconic thereby providing electrons for the respiratory ATP generation. Thus, P. putida partially uncoupled ATP formation from the availability of NADH. The quantitative analysis of intracellular pyridine nucleotides NAD(P)+ and NAD(P)H revealed elevated catabolic and anabolic reducing power during aerobic production of isobutanol. Additionally, the installation of micro‐aerobic conditions during production doubled the integral glucose‐to‐isobutanol conversion yield to 60 mgisobutanol gglucose–1 while preventing undesired carbon loss as 2‐ketogluconic acid.

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“…The ability of solventogenic Clostridium species to utilize a wide range of sugar substrates to produce acetone, butanol, and ethanol (ABE) makes them suitable candidates for generating transport fuels and chemical feedstocks from renewable biomaterials ( Jones and Woods, 1986 ; Grupe and Gottschalk, 1992 ; Ezeji et al, 2010 ). Among solvents produced by these Gram-positive, obligately anaerobic, spore-forming bacteria, butanol has drawn the most attention.…”

Section: Introductionmentioning

confidence: 99%

Ribozyme-Mediated Downregulation Uncovers DNA Integrity Scanning Protein A (DisA) as a Solventogenesis Determinant in Clostridium beijerinckii

Ujor

Lai

Okonkwo

et al. 2021

Front. Bioeng. Biotechnol.

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Carbon catabolite repression (CCR) limits microbial utilization of lignocellulose-derived pentoses. To relieve CCR in Clostridium beijerinckii NCIMB 8052, we sought to downregulate catabolite control protein A (CcpA) using the M1GS ribozyme technology. A CcpA-specific ribozyme was constructed by tethering the catalytic subunit of Escherichia coli RNase P (M1 RNA) to a guide sequence (GS) targeting CcpA mRNA (M1GSCcpA). As negative controls, the ribozyme M1GSCcpA–Sc (constructed with a scrambled GSCcpA) or the empty plasmid pMTL500E were used. With a ∼3-fold knockdown of CcpA mRNA in C. beijerinckii expressing M1GSCcpA (C. beijerinckii_M1GSCcpA) relative to both controls, a modest enhancement in mixed-sugar utilization and solvent production was achieved. Unexpectedly, C. beijerinckii_M1GSCcpA–Sc produced 50% more solvent than C. beijerinckii_pMTL500E grown on glucose + arabinose. Sequence complementarity (albeit suboptimal) suggested that M1GSCcpA–Sc could target the mRNA encoding DNA integrity scanning protein A (DisA), an expectation that was confirmed by a 53-fold knockdown in DisA mRNA levels. Therefore, M1GSCcpA–Sc was renamed M1GSDisA. Compared to C. beijerinckii_M1GSCcpA and _pMTL500E, C. beijerinckii_M1GSDisA exhibited a 7-fold decrease in the intracellular c-di-AMP level after 24 h of growth and a near-complete loss of viability upon exposure to DNA-damaging antibiotics. Alterations in c-di-AMP-mediated signaling and cell cycling likely culminate in a sporulation delay and the solvent production gains observed in C. beijerinckii_M1GSDisA. Successful knockdown of the CcpA and DisA mRNAs demonstrate the feasibility of using M1GS technology as a metabolic engineering tool for increasing butanol production in C. beijerinckii.

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Acetone-butanol fermentation revisited

Cited by 983 publications

References 250 publications

Exploring Co-fermentation of Glucose and Galactose using Clostridium acetobutylicum and Clostridium beijerinckii for Biofuels

Exploring Co-fermentation of Glucose and Galactose using Clostridium acetobutylicum and Clostridium beijerinckii for Biofuels

Micro‐aerobic production of isobutanol with engineered Pseudomonas putida

Ribozyme-Mediated Downregulation Uncovers DNA Integrity Scanning Protein A (DisA) as a Solventogenesis Determinant in Clostridium beijerinckii

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