Acetonitrile as solvent for protein interaction analysis

Arakawa, Tsutomu

doi:10.1016/j.ijbiomac.2018.03.174

Cited by 9 publications

(12 citation statements)

References 11 publications

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“…The CD wavelength scans could not be performed on protein after CCM binding since binding experiments used DMSO to solubilize CCM, which interfered with the measurement through strong absorbance of far-UV light. 38 CD spectra of all protein variants exhibited a double minimum at 222 and 208 nm, indicative of the α-helical content (Figure 5a and Table S1). Q displayed the lowest amount of helical content with minima −8000 ± 3000 and −10,000 ± 6000 deg•cm 2 •dmol −1 at 222 and 208 nm, respectively.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Supramolecular Assembly and Small-Molecule Binding by Protein-Engineered Coiled-Coil Fibers

et al. 2022

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The ability to engineer a solvent-exposed surface of self-assembling coiled coils allows one to achieve a higher-order hierarchical assembly such as nano-or microfibers. Currently, these materials are being developed for a range of biomedical applications, including drug delivery systems; however, ways to mechanistically optimize the coiled-coil structure for drug binding are yet to be explored. Our laboratory has previously leveraged the functional properties of the naturally occurring cartilage oligomeric matrix protein coiled coil (C), not only for its favorable motif but also for the presence of a hydrophobic pore to allow for smallmolecule binding. This includes the development of Q, a rationally designed pentameric coiled coil derived from C. Here, we present a small library of protein microfibers derived from the parent sequences of C and Q bearing various electrostatic potentials with the aim to investigate the influence of higher-order assembly and encapsulation of candidate small molecule, curcumin. The supramolecular fiber size appears to be well-controlled by sequence-imbued electrostatic surface potential, and protein stability upon curcumin binding is well correlated to relative structure loss, which can be predicted by in silico docking.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Supramolecular Assembly and Small-Molecule Binding by Protein-Engineered Coiled-Coil Fibers

et al. 2022

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“…CD experiments were performed using a JASCO J-715 circular dichroism spectrometer. To avoid the interference of DMSO, we first prepared the probe in acetonitrile (ACN) . The final concentration of ACN in an IAPP solution is 1%.…”

Section: Methodsmentioning

confidence: 99%

“…To avoid the interference of DMSO, we first prepared the probe in acetonitrile (ACN). 39 The final concentration of ACN in an IAPP solution is 1%. CD experiments were conducted after incubation of a 64 μM IAPP solution with and without the probe at 25 °C for 3 days with continuous shaking.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

A Fluorogenic Molecule for Probing Islet Amyloid Using Flavonoid as a Scaffold Design

et al. 2020

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“…Many nitriles are used in organic chemistry, including as solvents and synthons. Acetonitrile, the smallest organic nitrile, is widely used in reverse phase chromatography applications, 2 or as an organic solvent. 3 Polymer chemistry has afforded several important nitrile-containing polymers, including polyacrylonitrile, poly(acrylonitrile-co-butadiene-co-styrene), poly(styrene-co-acrylonitrile), and nitrile rubber.…”

Section: Introductionmentioning

confidence: 99%

Natural Products Containing the Nitrile Functional Group and Their Biological Activities

Scotti

Barlow²

2022

Natural Product Communications

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The importance of nitriles as a key class of chemicals with applications across the sciences is widely appreciated. The natural world is an underappreciated source of chemically diverse nitriles. With this in mind, this review describes novel nitrile-containing molecules isolated from natural sources from 1998 to 2021, as well as a discussion of the biological activity of these compounds. This study gathers 192 molecules from varied origins across the plant, animal, and microbial worlds. Their biological activity is extremely diverse, with many potential medicinal applications.

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Acetonitrile as solvent for protein interaction analysis

Cited by 9 publications

References 11 publications

Supramolecular Assembly and Small-Molecule Binding by Protein-Engineered Coiled-Coil Fibers

Supramolecular Assembly and Small-Molecule Binding by Protein-Engineered Coiled-Coil Fibers

A Fluorogenic Molecule for Probing Islet Amyloid Using Flavonoid as a Scaffold Design

Natural Products Containing the Nitrile Functional Group and Their Biological Activities

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