Acettc-Orcein: A New Stain-Fixative for Chromosomes

Abstract

A new stain-kative method for chromosomes, namely acetic-orcein, is described, which gives results that are equally good in fresh and permanent preparations. A 45% acetic and 1% orcein content is recommended as a standard solution. For salivary glands of Drosophila a 2% stain gives the best results, and with the two species D. melanogash and D . miranda the acetic strength has been raised to 70% with advantage. The addition of chloroform proves necessary for hardening in species of Sciara. Acetic-orcein is equally good for rapid chromosome counts. For root tips the addition of 1 cc. of N HC1 solution to 10 cc. of the standard solution to-

Initial Determination of Polymorphism andIn VitroConservation of SomeRamonda SerbicaandRamonda NathaliaePopulations from Albania, Macedonia and Bulgaria

“…The study carried out for haploid chromosome number n of young flower buds and somatic chromosome number 2n of germinated seeds. Sample slides were prepared from root tips and young flower buds with squash method (6) and the aceto-orcein method (13). Samples of material were fixed in the mixture 3:1 ethanol:acetic acid.…”

Value of Aceto-Orcein Squash Preparations in the Cytogenetic Study of Solid Tumours

“…Aceto-orcein, a chromatin stain that is highly specific for DNA, was introduced by La Cour [5] in 1941. Aceto-carmine and aceto-orcein smear and squash techniques have been used to study radiation response in carcinoma of the cervix [6] and for the detection of the early stages of uterine and breast cancer [7,8].…”

Plant Chromosome Preparations and Staining for Light Microscopic Studies

“…Staining Method According to La Cour [15] 1. Take root tips in vial containing 1N HCl and hydrolyze for few minutes at 60 °C in a water bath.…”

“…These banding methods are widely used for the identification of individual chromosomes in the karyotype, detection of structural changes in chromosomes, aneuploid identification, chromosome polymorphism and genome analysis of polyploids. Various chromosome banding methods that are reported in usage in plants and animal species are Q-, G-, C-, N-, F-, Hy-, R-and Ag-NOR-banding [15][16][17]. Among these banding methods, Q-and C-banding are widely used in plants.…”