Acetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium

Abstract

This work evaluated the effect of acetylated bacterial cellulose (ABC) substrates coated with urinary bladder matrix (UBM) on the behavior of retinal pigment epithelium (RPE), as assessed by cell adhesion, proliferation and development of cell polarity exhibiting transepithelial resistance and polygonal shaped-cells with microvilli. Acetylation of bacterial cellulose (BC) generated a moderate hydrophobic surface (around 65°) while the adsorption of UBM onto these acetylated substrates did not affect significantly the surface hydrophobicity. The ABS substrates coated with UBM enabled the development of a cell phenotype closer to that of native RPE cells. These cells were able to express proteins essential for their cytoskeletal organization and metabolic function (ZO-1 and RPE65), while showing a polygonal shaped morphology with microvilli and a monolayer configuration. The coated ABC substrates were also characterized, exhibiting low swelling effect (between 1.5-2.0 swelling/mm3), high mechanical strength (2048MPa) and non-pyrogenicity (2.12EU/L). Therefore, the ABC substrates coated with UBM exhibit interesting features as potential cell carriers in RPE transplantation that ought to be further explored. Therefore, the ABC substrates coated with UBM showed a great potential to be applied as cell carriers in RPE transplantation.

Bacterial nanocellulose as a corneal bandage material: a comparison with amniotic membrane

“…In this direction, BNC is also well known to support the culture of several cell types [50][51][52] including retinal pigment epithelial cells. 20,53 These reports, together with the findings presented here, suggest further uses of BNC as a cell carrier for corneal cell therapies. In the case of a BNC bandage supplemented with sensitive biological material (cells or bioactive molecules), the cryo-storage approach commonly used for preserving AM 54 could also be considered since our recent data demonstrate that BNC is stable at liquid nitrogen temperatures.…”

Latest Advances on Bacterial Cellulose‐Based Materials for Wound Healing, Delivery Systems, and Tissue Engineering

“…The requirements of an ideal carrier for RPE transplantation are very complex, therefore, the development of viable substrates has not yet been successful. Gonçalves et al . developed an acetylated bacterial cellulose (ABC) substrate coated with urinary bladder matrix for the adhesion and proliferation of RPE cells.…”

Development of novel bacterial cellulose composites for the textile and shoe industry

“…It also has high degree of polymerization, high water holding capacity and high moldability in situ (during fermentation) and ex situ (after fermentation) (Lee et al ., ). These unique properties have sustained the elevator pitch of several BC applications in the biomedical field, pulp and paper, composites and foods (Andrade et al ., ; Dourado et al ., ; Fortunato et al ., ; Gonçalves et al ., ; Padrão et al ., ).…”