Acetylcholine engages distinct amygdala microcircuits to gate internal theta rhythm

Bratsch-Prince, Joshua X; Warren, James W.; Jones, Grace C.; McDonald, Alexander J.; Mott, David D.

doi:10.1101/2023.02.26.530135

Cited by 6 publications

(8 citation statements)

References 87 publications

(153 reference statements)

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“…Unfortunately, there have been no anatomical investigations that have examined the expression of M3Rs in the BNC or studied cholinergic inputs to BNC CCK basket cells. However, electrophysiological studies have shown that BL CCK+ INs are activated by nicotinic receptors (Lee & Kim, 2019) and M3Rs (Bratsch‐Prince et al., 2023).…”

Section: Summary and Functional Considerationsmentioning

confidence: 99%

“…There is evidence that CCK+ INs are activated by nicotinic receptors (Lee & Kim, 2019) and muscarinic type 3 receptors (M3Rs) (Bratsch‐Prince et al., 2023). Some SOM+ INs are hyperpolarized by release of ACh, whereas others are depolarized (Bratsch‐Prince et al., 2023).…”

Section: Cholinergic Basal Forebrain Projections To the Bncmentioning

confidence: 99%

“…Indeed, PV INs have been implicated in generating theta oscillations in the BNC in previous studies, including in fear learning (Antonoudiou et al., 2022; Davis et al., 2017; Ozawa et al., 2020). However, a recent study found that endogenous release of ACh evokes prolonged theta oscillations in BLa local field potentials that depend upon the M3R‐mediated activation of the other type of perisomatic INs, CCK basket cells (Bratsch‐Prince et al., 2023). Unfortunately, there have been no anatomical investigations that have examined the expression of M3Rs in the BNC or studied cholinergic inputs to BNC CCK basket cells.…”

Section: Summary and Functional Considerationsmentioning

confidence: 99%

“…In a recent electrophysiological study, it was found that cortical inputs to the BLa were inhibited by presynaptic M4Rs, while at thalamic input inhibition depended on postsynaptic M3Rs triggering retrograde endocannabinoid signaling onto presynaptic CB1 cannabinoid receptors (Tryon et al., 2023). In addition, a recent electrophysiological investigation reported that CCK+ INs in the BLa can be activated via M3Rs (Bratsch‐Prince et al., 2023).…”

Section: Localization Of Muscarinic Cholinergic Receptors In the Bncmentioning

confidence: 99%

“…These investigations revealed that there are strong BF cholinergic inputs not only to PV+ INs but also to VIP+, SOM+, and CCK+ INs (Krabbe et al., 2019; Lee & Kim, 2019). There is evidence that CCK+ INs are activated by nicotinic receptors (Lee & Kim, 2019) and muscarinic type 3 receptors (M3Rs) (Bratsch‐Prince et al., 2023). Some SOM+ INs are hyperpolarized by release of ACh, whereas others are depolarized (Bratsch‐Prince et al., 2023).…”

Section: Cholinergic Basal Forebrain Projections To the Bncmentioning

confidence: 99%

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Functional neuroanatomy of basal forebrain projections to the basolateral amygdala: Transmitters, receptors, and neuronal subpopulations

McDonald

2024

J of Neuroscience Research

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The projections of the basal forebrain (BF) to the hippocampus and neocortex have been extensively studied and shown to be important for higher cognitive functions, including attention, learning, and memory. Much less is known about the BF projections to the basolateral nuclear complex of the amygdala (BNC), although the cholinergic innervation of this region by the BF is actually far more robust than that of cortical areas. This review will focus on light and electron microscopic tract‐tracing and immunohistochemical (IHC) studies, many of which were published in the last decade, that have analyzed the relationship of BF inputs and their receptors to specific neuronal subtypes in the BNC in order to better understand the anatomical substrates of BF‐BNC circuitry. The results indicate that BF inputs to the BNC mainly target the basolateral nucleus of the BNC (BL) and arise from cholinergic, GABAergic, and perhaps glutamatergic BF neurons. Cholinergic inputs mainly target dendrites and spines of pyramidal neurons (PNs) that express muscarinic receptors (MRs). MRs are also expressed by cholinergic axons, as well as cortical and thalamic axons that synapse with PN dendrites and spines. BF GABAergic axons to the BL also express MRs and mainly target BL interneurons that contain parvalbumin. It is suggested that BF‐BL circuitry could be very important for generating rhythmic oscillations known to be critical for emotional learning. BF cholinergic inputs to the BNC might also contribute to memory formation by activating M1 receptors located on PN dendritic shafts and spines that also express NMDA receptors.

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Section: Summary and Functional Considerationsmentioning

confidence: 99%

Section: Cholinergic Basal Forebrain Projections To the Bncmentioning

confidence: 99%

Section: Summary and Functional Considerationsmentioning

confidence: 99%

Section: Localization Of Muscarinic Cholinergic Receptors In the Bncmentioning

confidence: 99%

Section: Cholinergic Basal Forebrain Projections To the Bncmentioning

confidence: 99%

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Functional neuroanatomy of basal forebrain projections to the basolateral amygdala: Transmitters, receptors, and neuronal subpopulations

McDonald

2024

J of Neuroscience Research

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Scopolamine infusion in the basolateral amygdala after saccharin intake induces conditioned taste avoidance in rats

Torres-García,

Rodríguez-Nava,

Alcántara-Rivas

et al. 2024

Psychopharmacology

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Rationale Muscarinic receptor activity in the basolateral amygdala (BLA) is known to be involved in plasticity mechanisms that underlie emotional learning. The BLA is involved in the Attenuation of Neophobia, an incidental taste learning task in which a novel taste becomes familiar and recognized as safe. Objective Here we assessed the role of muscarinic receptor activity in the BLA in incidental taste learning. Methods Young adult male Wistar rats were bilaterally implanted with cannulas aimed at BLA. After recovery, rats were randomly assigned to either vehicle or muscarinic antagonist group, for each experiment. We tested the effect of specific and non-specific muscarinic antagonists administered either 1) 20 min before novel taste presentation; 2) immediately after novel taste presentation; 3) immediately after retrieval (the second taste presentation on Day 5 -S2-) or immediately after the fifth taste presentation on Day 8 (S5). Results Non-specific muscarinic receptor antagonist scopolamine infused prior to novel taste, while not affecting novel taste preference, abolished AN, i.e., the increased preference observed in control animals on the second presentation. When administered after taste consumption, intra-BLA scopolamine not only prevented AN but caused a steep decrease in the taste preference on the second presentation. This scopolamine-induced taste avoidance was not dependent on taste novelty, nor did it generalize to another novel taste. Targeting putative postsynaptic muscarinic receptors with specific M1 or M3 antagonists appeared to produce a partial taste avoidance, while M2 antagonism had no effect. Conclusion These data suggest that if a salient gustatory experience is followed by muscarinic receptors antagonism in the BLA, it will be strongly and persistently avoided in the future. The study also shows that scopolamine is not just an amnesic drug, and its cognitive effects may be highly dependent on the task and the structure involved.

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Transcriptomic correlates of state modulation in GABAergic interneurons: A cross-species analysis

Keijser,

Hertäg,

Sprekeler

2023

Preprint

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Inhibitory interneurons comprise many subtypes that differ in their molecular, anatomical and functional properties. In mouse visual cortex, they also differ in their modulation with an animal’s behavioural state, and this state modulation can be predicted from the first principal component (PC) of the gene expression matrix. Here, we ask whether this link between transcriptome and state-dependent processing generalises across species. To this end, we analysed seven single-cell and single-nucleus RNA sequencing datasets from mouse, human, songbird, and turtle forebrains. Despite homology at the level of cell types, we found clear differences between transcriptomic PCs, with greater dissimilarities between evolutionarily distant species. These dissimilarities arise from two factors: divergence in gene expression within homologous cell types and divergence in cell type abundance. We also compare the expression of cholinergic receptors, which are thought to causally link transcriptome and state modulation. Several cholinergic receptors predictive of state modulation in mouse interneurons are differentially expressed between species. Circuit modelling and mathematical analyses delineate the conditions under which these expression differences could translate into functional differences.

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Acetylcholine engages distinct amygdala microcircuits to gate internal theta rhythm

Cited by 6 publications

References 87 publications

Functional neuroanatomy of basal forebrain projections to the basolateral amygdala: Transmitters, receptors, and neuronal subpopulations

Functional neuroanatomy of basal forebrain projections to the basolateral amygdala: Transmitters, receptors, and neuronal subpopulations

Scopolamine infusion in the basolateral amygdala after saccharin intake induces conditioned taste avoidance in rats

Transcriptomic correlates of state modulation in GABAergic interneurons: A cross-species analysis

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