Acetylcholinesterase Activity in the Region of the Nematode Nerve Ring: Improved Histochemical Specificity Using Ultrasonic Pretreatment

Wright, Denis J.; Awan, Farooq A.

doi:10.1163/187529276x00616

Cited by 9 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1.4.34) activity was determined histochemically by the tetrazolium and coupled peroxidase methods (Pearse, 1972) using tyramine and tryptamine as substrates. Intact, cut and mildly sonicated (Wright & Awan, 1976) nematodes were used, with heat-treated (100°C for 5 min) and no-substrate controls. Some nematodes were also examined in the presence of and after incubation in the enzyme inhibitors pentachloronitrobenzene, nialamide or N-ethyl maleimide (at M).…”

Section: Methodsmentioning

confidence: 99%

Catecholaminergic structures in the nervous system of three nematode species, with observations on related enzymes

Wright¹,

Awan²

1978

Journal of Zoology

Self Cite

View full text Add to dashboard Cite

The formaldehyde fluorescence technique has been used to demonstrate amine‐specific fluorescence in the nervous system of three nematode species, Prionchulus punclatus Cobb, Panagrellus redivivus Goodey, and Aphelenchus avenae Bastian. Examination of some of the physical and chemical properties of this fluorescence has shown it to be due principally to the primary catecholamine dopamine. Dopamine and dopa decarboxylase were also detected biochemically in A. avenae. Dopamine has now been proposed as a putative neurotransmitter in a number of nematode species and the role of this and other biogenic amines in nematodes is discussed. Of the two principal enzymes involved in the metabolism of monoamines, catechol‐O‐methyltransferase was detected in both A. avenae and P. redivivus but monoamine oxidase could not be detected in these or other nematode species.

show abstract

Section: Methodsmentioning

confidence: 99%

Catecholaminergic structures in the nervous system of three nematode species, with observations on related enzymes

Wright¹,

Awan²

1978

Journal of Zoology

Self Cite

View full text Add to dashboard Cite

show abstract

Ultrastructural distribution of AChE in Catenula leptocephala (Nuttycombe, 1956)

Czubaj

1979

Histochemistry

View full text Add to dashboard Cite

Acetylcholinesterase activity (AChE, E.C. 3.1.1.7) was examined in different tissues of Catenula leptocephala (Nuttycombe, 1956). Eserine and iso-OMPA were used to distinguish AChE from non-specific cholinesterases (ChE, E.C. 3.1.1.8). The enzyme was located mainly in the brain neuropil, the peripheral nervous system, neuromuscular junctions, on the membrane of muscle cells and of cells with rhabdites. The distribution of the enzyme suggests that cholinergic transmission occurs in Catenula leptocephala, while simultaneously the presence of AChE on the membranes of muscle cells points to the receipt of cholinergic stimulation. The role of AChE in differentiation and maturation of cells with rhabdites is also discussed in this paper.

show abstract

Xiphinema americanum: Cholinesterase and biogenic amines in the nervous system

Hogger

Estey

Croll

1978

Experimental Parasitology

View full text Add to dashboard Cite

Acetylcholinesterase Activity in the Region of the Nematode Nerve Ring: Improved Histochemical Specificity Using Ultrasonic Pretreatment

Cited by 9 publications

References 0 publications

Catecholaminergic structures in the nervous system of three nematode species, with observations on related enzymes

Catecholaminergic structures in the nervous system of three nematode species, with observations on related enzymes

Ultrastructural distribution of AChE in Catenula leptocephala (Nuttycombe, 1956)

Xiphinema americanum: Cholinesterase and biogenic amines in the nervous system

Contact Info

Product

Resources

About