Acetylcholinesterase, butyrylcholinesterase, and tyrosinase inhibition studies and antioxidant activities of 33 Scutellaria L. taxa from Turkey

Şenol, Fatma Sezer; Orhan, Ilkay Erdogan; Yılmaz, Gülderen; Çiçek, Mehmet; Şener, Bilge

doi:10.1016/j.fct.2009.12.004

Cited by 84 publications

(51 citation statements)

References 29 publications

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“…Oboh et al, [26] had earlier reported that phenolic-rich extracts from some tropical leafy vegetables displayed potential neuroprotective properties by inhibiting ChEs activities and exhibiting antioxidant properties in vitro. Similarly, Senol et al, [42] reported ChEs inhibitory and antioxidant properties of aerial parts of thirty three taxa of Scutellaria species from Turkey relevant to their neuroprotective properties. Therefore, we could suggest that the phenolic contents of the extracts in this study could be responsible for their neuroprotective properties.…”

Section: Resultsmentioning

confidence: 96%

In vitro neuroprotective properties of some commonly consumed green leafy vegetables in Southern Nigeria

et al. 2016

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Green leafy vegetable is one of the major cuisines in Southern Nigeria and they are not only consumed for their palatability, but also for their nutritional and medicinal properties as reported in folklore. Notable among them are afang (Gnetum africanum), editan (Lasianthera africana) and utazi (Gongronema latifolium). In this study, we investigated the effect of aqueous extracts from afang, editan and utazi leaves on cholinesterases [acetylcho linesterase (AChE) and butyrylcholinesterase (BChE)] and monoamine oxidase (MAO) activities. Fe 2+ chelating abilities were also determined as an assessment of their neuroprotective potentials in vitro. We also assayed for their total phenol contents while the constituent phenolics were characterized using high performance liquid chromatography coupled with diode array detector (HPLC-DAD). The results revealed that the extracts inhibited AChE, BChE and MAO activities and also chelated Fe 2+ in concentration dependent manner. The HPLC-DAD char acterization showed that gallic, caffeic and ellagic acids and rutin were the dominant phenolic compounds in the extracts; nevertheless, utazi had the highest distribution of identified phenolics while afang had the least. The ability of the aqueous extracts of the vegetables to inhibit key enzymes (AChE, BChE and MAO) relevant to neu rodegeneration, as well chelate metal ion could help suggest their possible neuroprotective properties. These vegetables could be use as dietary intervention in the management of neurodegenerative diseases such as Alzheimer's and Parkinson's diseases.

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Section: Resultsmentioning

confidence: 96%

In vitro neuroprotective properties of some commonly consumed green leafy vegetables in Southern Nigeria

et al. 2016

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“…5,5´-Dithio-bis(2-nitrobenzoic)acid (DTNB, Sigma, St. Louis, MO, USA) was used for the measurement of the cholinesterase activity. All the other reagents and conditions were same as described in our earlier publication [18]. Briefly, in this method, 140 µL of 0.1 mM sodium phosphate buffer (pH 8.0), 20 µL of DTNB, 20 µL of test solution and 20 µL of AChE/BChE solution were added by multichannel automatic pipette (Gilson pipetman, France) in a 96-well microplate and incubated for 15 min at 25 °C.…”

Section: Determination Of Ache and Bche Inhibitory Activitymentioning

confidence: 99%

Evaluation of possible in vitro neurobiological effects of two varieties of Cupressus sempervirens (Mediterranean cypress) through their antioxidant and enzyme inhibition actions

Tümen¹,

Şenol²,

Orhan

2012

Turk J Bioch

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“…Butylated hydroxyanisole and butylated hydroxytoluene (BHT), for example, have been implicated in incidence of toxicity especially against animal DNA as well as liver damage and carcinogenesis (Dolatabadi & Kashanian, 2010). Consequently, there is a need for the discovery and development of novel/alternative natural antioxidants and AChEIs that are safe, affordable and effective at a global level (Senol et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Phenolic composition, antioxidant and anti-acetylcholinesterase activities of the TunisianScabiosa arenaria

Hlila¹,

Omri²,

Jannet

et al. 2013

Pharmaceutical Biology

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Context: There is a need for the discovery of novel natural antioxidants and acetylcholinesterase inhibitors (AChEIs) that are safe and effective at a global level. This is the first study on antioxidant and anti-acethylcholinesterase activity of Scabiosa arenaria Forssk (Dipsacaceae). Objective: The antioxidant potential and anti-acetylcholinesterase (AChE) activity of S. arenaria were investigated. Material and methods: The crude, ethyl acetate (EtOAc), butanol (n-BuOH) and water extracts prepared from flowers, fruits and stems and leaves of S. arenaria were tested to determine their total polyphenol content (TPC), total flavonoid content (TFC), total condensed tannin content (CTC) and their antioxidant activity by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2 0 -azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), reducing power and b-carotene bleaching inhibition activity. Anti-AChE activity was also determined. Results: EtOAc and n-BuOH fractions of fruits had both the highest (TPC) (269.09 mg gallic acid equivalents/g dry weight). The crude extract of stems and leaves had the highest TFC (10.9 mg quercetin equivalent/g dry weight). The n-BuOH fraction of stems and leaves had the highest CTC (489.75 mg catechin equivalents/g dry weight). The EtOAc fraction of flowers exhibit a higher activity in each antioxidant system with a special attention for DPPH assay (IC 50 ¼ 0.017 mg/mL) and reducing power (EC 50 ¼ 0.02 mg/mL). The EtOAc and n-BuOH fractions of stems and leaves showed strong inhibition of AChE (IC 50 ¼ 0.016 and 0.029 mg/ mL, respectively). Discussion and conclusions: These results suggest the potential of S. arenaria as a possible source of novel compounds and as an alternative antioxidant and AChEIs.

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Acetylcholinesterase, butyrylcholinesterase, and tyrosinase inhibition studies and antioxidant activities of 33 Scutellaria L. taxa from Turkey

Cited by 84 publications

References 29 publications

In vitro neuroprotective properties of some commonly consumed green leafy vegetables in Southern Nigeria

In vitro neuroprotective properties of some commonly consumed green leafy vegetables in Southern Nigeria

Evaluation of possible in vitro neurobiological effects of two varieties of Cupressus sempervirens (Mediterranean cypress) through their antioxidant and enzyme inhibition actions

Phenolic composition, antioxidant and anti-acetylcholinesterase activities of the TunisianScabiosa arenaria

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