Acetylcholinesterase from oat Seedlings. I. Preliminary biochemical characterization of the enzyme

Kęsy, Jacek; Tretyn, Andrzej; Łukasiewicz, H; Kopcewicz, Jan

doi:10.1007/bf02885378

Cited by 3 publications

(1 citation statement)

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“…These enzymes (specific acetylcholinesterases included) commonly appear in plant tissues (Miura andShih 1984, Tretyn andKendrick 1991). It is known that the degradation of choline esters in extracts may be effectively impeded by the inhibitors of AChE -eserine and neostigmine (K~sy et al 1991, Tretyn andKen-drick 1991). Since these inhibitors are structural analogues of ACh they undergo simultaneous precipitation with choline esters.…”

Section: Resultsmentioning

confidence: 99%

Isolation, purification and identification of acetylcholine in Pharbitis nil seedlings

Tretyn¹,

Łukasiewicz-Rutkowska²,

Kopcewicz³

1997

Acta Physiol Plant

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Acetylcholine (ACh), a well known animal neurotransmitter was isolated from tissues of Pharbitis nil using five different methods. Its presence in plant extract was confirmed by infrared spectroscopy and mass spectrometry. For quantitative estimation of ACh in P. nil seedlings pyrolysis-gas chromatography was applied. The presence of ACh was found in all organs of the examined plant: seeds, shoot apex, cotyledons, leaves, shoots and roots. However, the highest level of the investigated substance was noted in the youngest growing parts. In 5-dayold etiolated seedlings they were cotyledons, whereas in 14 day-old green plants -shoot apex and young leaves.

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Section: Resultsmentioning

confidence: 99%