Acetylcholinesterase from the brain of Monopterus albus as detection of metal ions

Khalidi, Siti Aishah Muhammad; Sabullah, Mohd Khalizan; Sani, Suraya Abdul; Ahmad, Siti Aqlima; Shukor, Mohd Yunus; Jaafar, Izazy Nur Mohd; Gunasekaran, Baskaran

doi:10.1088/1742-6596/1358/1/012028

Cited by 5 publications

(5 citation statements)

References 33 publications

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“…To further discuss, in the study done by Sabullah et al, (2014) on P. javanicus liver tissue, ChE was found to be specific towards BTC with (V max /K m ) higher than ATC and PTC. However, a study on M. albus brain tissue by Khalidi et al (2019) found that ATC had the highest (V max /K m ), similar to the result obtained in this study. These results proved that different parts of organs and different species of fish incorporated ChE with different substrate specificity of either ATC, BTC or PTC.…”

Section: Substrate Specificity Profilingsupporting

confidence: 90%

Profiling of Cholinesterase Extracted from The Brain Tissue of Diodon hystrix and Its Inhibition Reaction Towards Carbamates

Haris¹,

Nordin²,

Mustapa³

et al. 2020

JST

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Diodon hystrix, commonly known as spot-fin porcupine fish is a salt-water fish belonging to the Diodontidae family. It is widely distributed in Sabah wet market due to its commercial value. This study exploits the effectiveness of Cholinesterase (ChE) obtained from the brain tissue of D. hystrix in detecting carbamates inhibitory activities. Carbamate pesticides known to inhibit ChE and toxic towards living organisms can contaminate the water bodies. By using diethylaminoethanol (DEAE) Sepharose ion exchange chromatography, a total of 40% recovery yield of ChE was obtained with a 165.77 purification fold. Furthermore, the ChE showed a high affinity towards acetylthiocholine iodide (ATC) with an optimum activity at pH 7.45 and temperature ranging from 20 to 40℃. Among five different types of carbamates, methomyl was found to have the highest percentage of inhibition analyzed using ChE inhibitory assay, followed by carbofuran, bendiocarb, carbaryl and propoxur with >85% inhibition rate. The results concluded that ChEs extracted from the brain tissue of D. hystrix are applicable to be used as a bioindicator in detecting the presence of carbamates.

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Section: Substrate Specificity Profilingsupporting

confidence: 90%

Profiling of Cholinesterase Extracted from The Brain Tissue of Diodon hystrix and Its Inhibition Reaction Towards Carbamates

Haris¹,

Nordin²,

Mustapa³

et al. 2020

JST

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show abstract

“…Elman reagent known as 5,5'-dithio-bis-2nitrobenzoic acid or DTNB was used as a chromogen to indicates the level of cholinesterase activity. Acetylthiocholine iodide; ATC, was selected as a preferred synthetic substrate due to the highest ratio of the effective coefficient, which was recorded in a previous study [10]. Both chromogen and substrate are Sigma-Aldrich brands.…”

Section: Chemicalsmentioning

confidence: 99%

Enzyme inhibition-based biosensors using Acetylcholinesterase from Monopterus albus for detection of carbamates contamination

Khalidi

Sabullah

Gansau

et al. 2022

J. Phys.: Conf. Ser.

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Insecticides are an example of an environmental contaminant that can cause harmful effects on various types of organisms. Implementation of continuous monitoring program is needed to ensure the level of contamination can be controlled. In this present study, acetylcholinesterase (AChE) from the brain of Monopterus albus was elucidated to determine the potential alternative source of biosensor kit, which is sensitive towards various insecticides, especially carbamates. AChE from M. albus brain was extracted and purified through ammonium sulfate precipitation followed by procainamide-based affinity chromatography. Carbamate insecticides were used, such as bendiocarb, carbaryl, carbofuran, methomyl, and propoxur, to test their ability to inhibit AChE activity. Bendiocarb and methomyl show the capability to inhibit almost half of the enzyme activity at 51.05 and 51.20 %, respectively, while carbaryl, propoxur, and carbofuran inhibit 43.03, 42.80, and 15.06 %, respectively. Bendiocarb and methomyl were selected, and M. albus AChE was separately exposed with different concentrations of those carbamates and half maximal inhibitory concentration; IC50 was determined at 0.874 and 1.639 ppm, respectively. A field trial was conducted by testing the enzyme with various vegetable samples. All samples show no significant effect on AChE activity, meaning there was no existence of insecticides in each sample (p > 0.05). This study could be used as an alternative source for developing biosensor kits for the environmental monitoring program.

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“…The brain was homogenised in phosphate buffer (0.1 M, pH 7.5) with the ratio of 1:5 (weight of brain: volume of buffer) followed by 10 minutes centrifugation at the 10,000xg in refrigerated condition. Protein precipitation and affinity purification were conducted based on the method previously developed by Khalidi et al [17] using ammonium sulfate concentrations ranging from 20 to 70% of salt. The pellet was solubilised (1:4 weight per volume of extraction buffer) and overnight desaltation.…”

Section: Specimen Preparation and Ache Purificationmentioning

confidence: 99%

Preliminary Screening and Comparison of Heavy Metal Pollution in Two Malaysia Class II River Using Acetylcholinesterase from Monopterus Albus

Khalidi

Sabullah

Abdullah

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Development in Malaysia is in line with positive economic growth. The situations have led to the improvement of industrial and agricultural activities that produce high-quality products of a global quality, which has a significant impact on the income of the local people. However, the activity also contributed to river pollution, where the industrial and agricultural wastes were discharged to nearby water sources, whether intentionally or not. The residues containing heavy metals are of concern because their concentration can negatively affect the environment. Furthermore, their ability to be treated or remedied is very limited. Biosensor acetylcholine, AChE extracted and purified from Monopterus albus brain, acts as an alternative biosensor to rapidly detect the presence of heavy metals with a simple application. The tests were conducted at ten different location points from the upstream to the downstream of Bentong River and Terengganu River, classified as a Class II river by the Department of Environment Malaysia. Bentong River and Terengganu River showed that five and two samples from different location points were able to inhibit AChE activity by more than 10%, respectively. The three samples with the highest inhibition were selected for the secondary screening through identification and the heavy metal concentrations determination using ICP-OES. All samples showed heavy metals such as copper, nickel, lead, arsenic, silver, chromium, and zinc at different concentrations and synergistically affected AChE activity. The use of AChE as a biosensor is able to detect and characterize pollution levels in both rivers that differ in the level of local development, such as industrial activities and population density.

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Acetylcholinesterase from the brain of Monopterus albus as detection of metal ions

Cited by 5 publications

References 33 publications

Profiling of Cholinesterase Extracted from The Brain Tissue of Diodon hystrix and Its Inhibition Reaction Towards Carbamates

Profiling of Cholinesterase Extracted from The Brain Tissue of Diodon hystrix and Its Inhibition Reaction Towards Carbamates

Enzyme inhibition-based biosensors using Acetylcholinesterase from Monopterus albus for detection of carbamates contamination

Preliminary Screening and Comparison of Heavy Metal Pollution in Two Malaysia Class II River Using Acetylcholinesterase from Monopterus Albus

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