Acetylcholinesterase–ISFET based system for the detection of acetylcholine and acetylcholinesterase inhibitors

Metabolic rate constants: Some computational aspects

“…Our analysis shows that model (11) deserves to be included in the comparison of the two models (2), (4). We shall further show that under the conditions K ≫ 1, k −1 ≪ k 2 the three models (2), (4) and (11) do indeed provide almost identical results and that the substrate solution of the MM-model may provide a good approximation even though e 0 ≪ s 0 does not necessarily hold.…”

Cholinesterase Inhibitors

“…An ionsensitive field effect transistor with AChE bound to its gate showed sensitivity of up to 10 −5 M of ACh and the ability to detect physostig mine concentrations in the range of 10 −4 to 10 −7 M, based on the decrease in the recorded current in comparison to the response without the inhibitor (Hai et al, 2006). In another study, AChE was bound to a floating gate derived field effect transistor, with a response range of 10 −2 to 10 −9 M ACh and achieved a sensitivity to 10 −8 M physostigmine (Goykhman et al, 2009).…”

ZnO nanostructures in enzyme biosensors

“…The application of ISFETs as ENFET biosensors is realized when pH changes near the gate are mediated by specific enzymes, in the presence of their substance in a dose-dependent manner [104]. Small molecules including glucose [105], urea [106][107][108], acetylcholine [109], creatinine [107] and cholesterol [110] have been widely investigated using ENFETs. Most recent research has focused on silicon nanomaterials [111][112][113], Au nanowires [114] and CNTs [115][116][117], and a few studies have investigated metal oxide semiconductors.…”