Acetylcholinesterase, Specific Acetylcholinesterase and Total Protein Concentrations in the Brain Regions of Broiler Chickens Fed Dietary Monosodium Glutamate

Abstract

The study was carried out to examine the effect of varied levels of dietary monosodium glutamate on acetylcholinesterase, specific acetylcholinesterase and total protein concentrations in the brain regions of broiler chickens. Three hundred (300) day – old unsexed Abor – acre chickens were randomly assigned to diets: A, B, C, D, E and F containing 0.00, 0.25, 0.50, 0.75, 1.00 and 1.25 g/kg MSG respectively. Each treatment was replicated 5 times with 10 birds per replicate. The birds were fed ad – libitum and provided with clean water for 8 weeks (56 days) after which 2 birds per replicates were slaughtered. The brains were removed, dissected into different regions comprising of the olfactory lobe, pineal body, optic lobe, cerebellum and the medulla oblongata. The different parts of the brain were homogenized to determine the acetylcholinesterase and total protein which were also used in the assessment of the specific acetylcholinesterase of the brain. No significant differences were observed in the acetylcholinesterase activity of the olfactory lobe, pineal body, optic lobe, cerebellum except for the medulla. Likewise, the dietary monosodium glutamate did not influence the activities of the total protein and specific acetylcholinesterase of the olfactory lobe portion of the brain. The dietary monosodium glutamate exerted significant effects on the total protein of other brain parts studied and which invariably resulted in significant changes in the specific acetylcholinesterase of the optic lobe, cerebellum and medulla except for the optic lobe. This study revealed that monosodium glutamate added to broilers diet above 0.75 g/kg significantly altered the concentration of the brain acetylcholinesterase, total protein and specific acetylcholinesterase thereby impaired brain functions.

Hypophyseal acetylcholinesterase, and growth hormones of broiler chicken fed Ficus carica and vitamin C supplemented diets

“…This action could promote cholinergic neurotransmission and thus availability of acetylcholine at the synapse [19]. The insignificant effect in the AChE activities of the pineal body of the broiler chickens showed that dietary Ficus carica and vitamin C at the supplemental levels as well as the interaction of the two treatments and levels did not interfere with melatonin production thereby did not affect negatively the modulation of sleeping pattern and behavior of the birds [20]. Moreso, the significant effect of the activities of the AChE of the olfactory lobes of the broilers indicated that their sense of smell was not also negatively affected by the interactions which are attributed to the fact that birds have the least sense of smell and do not use smell extensively to make most of their decisions [21].…”

Improving Behavior, Immune Statue and Growth of Broilers by Adding Some Neurotransmitters in Drinking Water and Hero Flowers (Alcea Kurdica) in Diets

“…The experimental feeds were prepared in Evan Company and contained starter (1-10), grower (11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25), and finisher (26-42) days: 3097, 2990, and 2906 kcal/kg metabolizable energy, and 22.97, 21.50, and 20.78 % crude protein respectively. Chicks received 24 hours of light (L) during the 1 st week.…”

“…Body growth: Body weight, weight gain, feed intake, Feed Conversion Ratio (FCR), mortality and feasibility were measured during 42 days according to Olarotimi, and Adu [14].…”