Acetylene reduction (N2-fixation) by Enterobacteriaceae isolated from industrial wastewaters and biological treatment systems

Neilson, Alasdair H.; Allard, Ann‐Sofie

doi:10.1007/bf02660120

Cited by 3 publications

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References 37 publications

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“…3). Added glucose and acetate increased the N 2 -fixing activity more than sevenfold over 24 h. Although the Enterobacteriaceae, particularly Klebsiella pneumoniae, are metabolically versatile (20), it is likely that the glucose was the major driver of increased N 2 fixation. Bruce and Clark (4) observed that the AR rate of K. pneumoniae isolates growing on Kraft mill effluent in an aerated stabilization basin (lagoon) was greatly stimulated by 5 g of glucose liter…”

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Coliform Bacteria and Nitrogen Fixation in Pulp and Paper Mill Effluent Treatment Systems

Gauthier

Neufeld

Driscoll

et al. 2000

Appl Environ Microbiol

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The majority of pulp and paper mills now biotreat their combined effluents using activated sludge. On the assumption that their wood-based effluents have negligible fixed N, and that activated-sludge microorganisms will not fix significant N, these mills routinely spend large amounts adding ammonia or urea to their aeration tanks (bioreactors) to permit normal biomass growth. N 2 fixation in seven Eastern Canadian pulp and paper mill effluent treatment systems was analyzed using acetylene reduction assays, quantitative nitrogenase (nifH) gene probing, and bacterial isolations. In situ N 2 fixation was undetectable in all seven bioreactors but was present in six associated primary clarifiers. One primary clarifier was studied in greater detail. Approximately 50% of all culturable cells in the clarifier contained nifH, of which >90% were Klebsiella strains. All primaryclarifier coliform bacteria growing on MacConkey agar were identified as klebsiellas, and all those probed contained nifH. In contrast, analysis of 48 random coliform isolates from other mill water system locations showed that only 24 (50%) possessed the nifH gene, and only 13 (27%) showed inducible N 2 -fixing activity. Thus, all the pulp and paper mill primary clarifiers tested appeared to be sites of active N 2 fixation (0.87 to 4.90 mg of N liter ؊1 day ؊1 ) and a microbial community strongly biased toward this activity. This may also explain why coliform bacteria, especially klebsiellas, are indigenous in pulp and paper mill water systems.

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“…Indeed, the presence of N 2 -fixing members of the Enterobacteriaceae, including Klebsiella sp. strains, in pulp and paper mill water systems has long been known (2,4,6,8,9,15,18,(20)(21)(22).…”

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confidence: 99%

Coliform Bacteria and Nitrogen Fixation in Pulp and Paper Mill Effluent Treatment Systems

Gauthier

Neufeld

Driscoll

et al. 2000

Appl Environ Microbiol

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Composition of nifH in a wastewater treatment system reliant on N2 fixation

Bowers

Reid

Lloyd‐Jones

2008

Appl Microbiol Biotechnol

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High levels of nitrogen fixation have been observed in the wastewaters of pulp and paper mills. In this study, we show that nitrogen fixation in a model pulp and paper wastewater treatment system is supported by a high density of nifH sequences that are of low diversity. Quantitative PCR revealed a ratio of nifH to 16S rDNA of 1.14 +/- 0.76 which shows that very high levels of the nifH gene were enriched to support the high rates of nitrogen fixation that occur in this wastewater. Changes in wastewater composition and dissolved oxygen levels did not affect the nifH levels and allowed stable wastewater treatment. The nifH sequences identified display a similar profile to those seen in forest soil environments where nifH sequences derived from alpha-proteobacteria and beta-proteobacteria are also prevalent.

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Acetylene reduction (N2-fixation) by Enterobacteriaceae isolated from industrial wastewaters and biological treatment systems

Cited by 3 publications

References 37 publications

Coliform Bacteria and Nitrogen Fixation in Pulp and Paper Mill Effluent Treatment Systems

Coliform Bacteria and Nitrogen Fixation in Pulp and Paper Mill Effluent Treatment Systems

Composition of nifH in a wastewater treatment system reliant on N2 fixation

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