We report a multi-resonant THz metamaterial perfect absorber-based biosensor in the working frequency range of 0.2 to 4THz for the sensing of microorganisms such as fungi, yeast, and pesticides. Nearly100% absorption is realized at f1 = 1.7T Hz, f2 = 2.8T Hz, f3 = 3.2T Hz, f4 = 3.5T Hz. To obtain optimum performance of the MPA, a mapping of amplitudes and shifts in the absorption resonance peaks with different structural parameters of the resonators is carried out. Investigations of the effect of the incidence angle of the THz wave on the absorption spectra of the MPA are also carried out. It is found that the incidence angle does not impact the stability of the lower resonance absorption peak (1.79T Hz). To better understand the mechanism of MPA, an equivalent circuit model using Advance Design System(ADS) software is developed. The calculated results through the model show similar trends as obtained in the simulations using CST. A significant frequency shift in the resonances is obtained with the application of microorganisms such as Penicillium chrysogenum (fungi), yeast, and pesticides (Imidacloprid, N, N-Diethyldithiocarbamate sodium salt trihydrate, Daminozide, N, N-Diethyldithiocarbamate sodium salt hydrate, and Dicofol) as testing analytes. The THz MPA-based biosensor has great potential to detect a very small change in the effective dielectric constant of the MPA environment therefore it can offer huge opportunities in label-free biosensing for future biomedical applications.