Acibenzolar-S-methyl activates stomatal-based defense systemically in Japanese radish by inducing peroxidase-dependent reactive oxygen species production

Abstract

12Acibenzolar-S-methyl (ASM) is a well-known plant activator, which is a synthetic analog of salicylic 13 acid (SA). Recently, copper fungicides and antibiotics are major strategies for controlling bacterial 14 diseases. However, resistant strains have already been found. Therefore, there is an increasing 15 demand for sustainable new disease control strategies. We investigated the ASM disease control 16 effect against Pseudomonas cannabina pv. alisalensis (Pcal), which causes bacterial blight on 17Japanese radish. In this study, we demonstrated that ASM effectively suppressed Pcal disease 18 symptom development associated with reduced bacterial populations on Japanese radish leaves. 19 Interestingly, we also demonstrated that ASM activated systemic acquired resistance (SAR), 20including stomatal-based defense, not only on ASM treated leaves, but also on untreated upper and 21 lower leaves. Reactive oxidative species (ROS) are essential second messengers in stomatal-based 22 defense. We found that ASM induced stomatal closure by inducing ROS production through 23 peroxidase. These results indicate that stomatal closure induced by ASM treatment is effective for 24preventing Pcal pathogen invasion into plants, and in turn reduction of disease development. 25 26

Coronatine contributesPseudomonas cannabinapv.alisalensisvirulence by overcoming both stomatal and apoplastic defenses in dicot and monocot plants

“…Pcal also produces COR [41]. Furthermore, acibenzolar-S-methyl (ASM), a well-known plant defense activator, protects cabbage and Japanese radish from Pcal KB211 by activating stomatal-based defense [42–44]. These results imply that overcoming stomatal-based defense is important for successful Pcal infection.…”