Acid adaptive response of Alicyclobacillus acidoterrestris: A strategy to survive lethal heat and acid stresses

Zhao, Ning; Xu, Junnan; Jiao, Lei; Liu, Miaomiao; Zhang, Tong; Li, Jun; Wei, Xinyuan; Fan, Mingtao

doi:10.1016/j.foodres.2022.111364

Cited by 12 publications

(4 citation statements)

References 50 publications

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“…3 A). High pppGpp pool leading to an increase in survival was also observed in acid-stressed Oenococcus oeni (Qi et al 2021 ) and Alicyclobacillus acidoterrestris (Zhao et al 2022 ).…”

Section: Resultsmentioning

confidence: 86%

Methionine addition improves the acid tolerance of Lactiplantibacillus plantarum by altering cellular metabolic flux, energy distribution, lipids composition

Meng

Yao

et al. 2022

Stress Biology

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This paper reported a wine-derived lactic acid bacterium, Lactiplantibacillus plantarum XJ25, which exhibited higher cell viability under acid stress upon methionine supplementation. Cellular morphology and the composition of the cytomembrane phospholipids revealed a more solid membrane architecture presented in the acid-stressed cells treated with methionine supplementation. Transcriptional analysis showed L. plantarum XJ25 reduced methionine transport and homocysteine biosynthesis under acid stress. Subsequent overexpression assays proved that methionine supplementation could alleviate the cell toxicity from homocysteine accumulation under acid stress. Finally, L. plantarum XJ25 employed energy allocation strategy to response environmental changes by balancing the uptake methionine and adjusting saturated fatty acids (SFAs) in membrane. These data support a novel mechanism of acid resistance involving methionine utilization and cellular energy distribution in LAB and provide crucial theoretical clues for the mechanisms of acid resistance in other bacteria.

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“…3 A). High pppGpp pool leading to an increase in survival was also observed in acid-stressed Oenococcus oeni (Qi et al 2021 ) and Alicyclobacillus acidoterrestris (Zhao et al 2022 ).…”

Section: Resultsmentioning

confidence: 86%

Methionine addition improves the acid tolerance of Lactiplantibacillus plantarum by altering cellular metabolic flux, energy distribution, lipids composition

Meng

Yao

et al. 2022

Stress Biology

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“…Maintaining energy balance is essential for the survival of bacteria [54]. In this study, it was found that CPF-Fe may interfere with the energy metabolism of bacteria by affecting three pathways: glycolysis, the TCA cycle, and oxidative phosphorylation.…”

Section: Energy Metabolism Disordermentioning

confidence: 90%

Multi-Omics Study on Molecular Mechanisms of Single-Atom Fe-Doped Two-Dimensional Conjugated Phthalocyanine Framework for Photocatalytic Antibacterial Performance

Diao,

Duan,

Wang

et al. 2024

Molecules

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Currently, photocatalysis of the two-dimensional (2D) conjugated phthalocyanine framework with a single Fe atom (CPF-Fe) has shown efficient photocatalytic activities for the removal of harmful effluents and antibacterial activity. Their photocatalytic mechanisms are dependent on the redox reaction—which is led by the active species generated from the photocatalytic process. Nevertheless, the molecular mechanism of CPF-Fe antimicrobial activity has not been sufficiently explored. In this study, we successfully synthesized CPF-Fe with great broad-spectrum antibacterial properties under visible light and used it as an antibacterial agent. The molecular mechanism of CPF-Fe against Escherichia coli and Salmonella enteritidis was explored through multi-omics analyses (transcriptomics and metabolomics correlation analyses). The results showed that CPF-Fe not only led to the oxidative stress of bacteria by generating large amounts of h+ and ROS but also caused failure in the synthesis of bacterial cell wall components as well as an osmotic pressure imbalance by disrupting glycolysis, oxidative phosphorylation, and TCA cycle pathways. More surprisingly, CPF-Fe could disrupt the metabolism of amino acids and nucleic acids, as well as inhibit their energy metabolism, resulting in the death of bacterial cells. The research further revealed the antibacterial mechanism of CPF-Fe from a molecular perspective, providing a theoretical basis for the application of CPF-Fe photocatalytic antibacterial nanomaterials.

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“…To better understand the acid stress response mechanisms, a comparative transcriptomic and metabolic analysis of A. acidoterrestris was performed. The transcriptomic data of A. acidoterrestris with the same experimental setup as described in this work were reported in our previous study ( 47 ) and have been uploaded to the Sequence Read Archive (SRA) database (accession no. PRJNA742261 ).…”

Section: Methodsmentioning

confidence: 99%

Transcriptomic and Metabolomic Profiling Uncovers Response Mechanisms of Alicyclobacillus acidoterrestris DSM 3922 ^T to Acid Stress

Zhao

Meng

et al. 2023

Microbiol Spectr

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Acid adaptive response of Alicyclobacillus acidoterrestris: A strategy to survive lethal heat and acid stresses

Cited by 12 publications

References 50 publications

Methionine addition improves the acid tolerance of Lactiplantibacillus plantarum by altering cellular metabolic flux, energy distribution, lipids composition

Methionine addition improves the acid tolerance of Lactiplantibacillus plantarum by altering cellular metabolic flux, energy distribution, lipids composition

Multi-Omics Study on Molecular Mechanisms of Single-Atom Fe-Doped Two-Dimensional Conjugated Phthalocyanine Framework for Photocatalytic Antibacterial Performance

Transcriptomic and Metabolomic Profiling Uncovers Response Mechanisms of Alicyclobacillus acidoterrestris DSM 3922 ^T to Acid Stress

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Acid adaptive response of Alicyclobacillus acidoterrestris: A strategy to survive lethal heat and acid stresses

Cited by 12 publications

References 50 publications

Methionine addition improves the acid tolerance of Lactiplantibacillus plantarum by altering cellular metabolic flux, energy distribution, lipids composition

Methionine addition improves the acid tolerance of Lactiplantibacillus plantarum by altering cellular metabolic flux, energy distribution, lipids composition

Multi-Omics Study on Molecular Mechanisms of Single-Atom Fe-Doped Two-Dimensional Conjugated Phthalocyanine Framework for Photocatalytic Antibacterial Performance

Transcriptomic and Metabolomic Profiling Uncovers Response Mechanisms of Alicyclobacillus acidoterrestris DSM 3922 T to Acid Stress

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Transcriptomic and Metabolomic Profiling Uncovers Response Mechanisms of Alicyclobacillus acidoterrestris DSM 3922 ^T to Acid Stress