Acid and Heavy Metal Tolerant Bacillus sp. from Rat-Hole Coal Mines of Meghalaya, India

Ka-ot, Augustine Lamin; Banerjee, Soumya; Haldar, Gopinath; Joshi, S. R.

doi:10.1007/s40011-017-0856-x

Cited by 11 publications

(4 citation statements)

References 13 publications

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“…MGI101, were isolated from AMD sites. They were Gram-positive, rod-shaped, endospore-forming strictly aerobic bacteria, profiles that matched those of other bacteria previously isolated from similar niche environments (Gupta et al 2012;Ka-ot et al 2017;Liu et al 2013;Park et al 2012;Wichlacz et al 1986). These two isolates showed tremendous resistance and biosorptive abilities toward the target elements employed in this study as indicated by the plate growth and ICP-OES analysis.…”

Section: Discussionsupporting

confidence: 76%

Exploring the application of biostimulation strategy for bacteria in the bioremediation of industrial effluent

Ijoma

Selvarajan

Oyourou³

et al. 2019

Ann Microbiol

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The purpose of this study was to isolate and characterise toxic element-resistant bacteria from acid mine drainage water and to apply them in the bioremediation of industrial effluent, as well as to identify optimal effluent:nutrient concentration for onsite biostimulation strategy. Wastewater samples were collected from acid mine drainage and industry. Inductively coupled plasma optical emission spectroscopy (ICP-OES) was employed for elemental composition analysis. Isolated bacterial strains were characterised using molecular methods. Bioremediation assays were employed to determine the extent of bacterial tolerance and removal of toxic elements using a biostimulation strategy employing minimal salt medium (MSM) at varied concentrations and positive and negative controls of only MSM and industrial effluent, respectively. Two bacterial strains demonstrated resistance to toxic elements, Bacillus sp. MGI101 and Lysinibacillus sp. MGI102 both isolated from the AMD sites. However, no observable growth of toxic metal-resistant bacteria was obtained from the industrial effluents. Bacterial strains MGI101 and MGI102 demonstrated high resistance to target toxic elements during the screening and tolerance assays. Remarkably, Bacillus sp. MGI101 demonstrated greater ability to remove toxic elements including arsenic, chromium, zinc, copper and aluminium in undiluted solutions of the industrial effluent, with its highest removal capacity observed at > 60% for arsenic and aluminium. Both Bacillus sp.MGI101 and Lysinibacillus sp. MGI102 demonstrated varied abilities for the removal of toxic elements from dilution concentration of effluent mixed with MSM. However, the optimal dilution ratio observed in this experiment was 5:15 (effluent:MSM). Overall results demonstrated that isolated bacterial strains have the potential to be employed in bioremediation programmes of acid mine drainages and multi-element contaminated water.

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Section: Discussionsupporting

confidence: 76%

Exploring the application of biostimulation strategy for bacteria in the bioremediation of industrial effluent

Ijoma

Selvarajan

Oyourou³

et al. 2019

Ann Microbiol

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“…Biochar can reduce the transportation of Cd by surface complexation, precipitation, and ion exchange 48 . Bacillus strains isolated in the study of Ka-Ot et al 49 . have strong tolerance to 800 mg•L −1 Cd, probably because they can accumulate Cd at the glutamic acid carboxyl of peptidoglycan, which is associated with a large number of granular Cd deposits in the cell wall 50 .…”

Section: Discussionmentioning

confidence: 99%

Effects of biochar and biofertilizer on cadmium-contaminated cotton growth and the antioxidative defense system

Zhu

Wang

et al. 2020

Sci Rep

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Consistent use of large amounts of fertilizers, pesticides, and mulch can cause the accumulation of harmful substances in cotton plants. Among these harmful substances, cadmium (Cd), an undegradable element, stands out as being particularly highly toxic to plants. The objective of this study was to evaluate the ability of biochar (3%) and biofertilizer (1.5%) to decrease Cd uptake, increase cotton dry weight, and modulate the activities of photosynthetic and peroxidase (POD), superoxide dismutase (SOD), catalase enzyme (CAT) in cotton (Gossypium hirsutum L.) grown in Cd-contaminated soil (0, 1, 2, or 4 mg Cd kg−1 soil) in pots. These studies showed that, as expected, exogenous Cd adversely affects cotton chlorophyll and photosynthesis. However, biochar and biofertilizer increased cotton dry weight by an average of 16.82% and 32.62%, respectively. Meanwhile, biochar and biofertilizer decreased the accumulation of Cd in cotton organs, and there was a significant reduction in the amount of Cd in bolls (P < 0.05). Biochar and biofertilizer have a positive impact on cotton chlorophyll content, net photosynthesis, stomatal conductance, transpiration rate, and intercellular CO2 concentration. Thus, the addition of biochar and biofertilizer promote cotton growth. However, biochar and biofertilizer increased the SOD activity of leaves (47.70% and 77.21%), CAT activity of leaves (35.40% and 72.82%), SOD activity of roots (33.62% and 39.37%), and CAT activity of roots (36.91% and 60.29%), respectively, and the addition of biochar and biofertilizer decreased the content of MDA and electrolyte leakage rate. Redundancy analyses showed that biochar and biofertilizer also improved SOD and POD activities by reducing the heavy metal-induced oxidative stress in cotton and reducing Cd uptake in cotton organs. Therefore, biochar and biofertilizer have a positive effect on the growth of cotton.

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“…Shylla et al (3) reported that B. pseudomycoides KH-12A showed 400 ppm tolerance to Fe and 730 ppm to Mn, obviously lower than those of B. pseudomycoides X8. The MTC values of 90-1000 ppm against Fe (3, 39) and MTC of 120-800 ppm against Mn (3) have been reported in other Bacillus species, while the isolates P. megaterium X11 tolerated 800 mg/L of Fe and 2000 mg/L of Mn, and B. subtilis G2 resisted 600 mg/L of Fe and 2000 mg/L of Mn.…”

Section: Resultsmentioning

confidence: 67%

Comparative Genomic Analysis of Metal-Tolerant Bacteria Reveals Significant Differences in Metal Adaptation Strategies

Chen,

Zhang,

Zhang

et al. 2024

Preprint

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Metal-tolerant bacteria have been commercially used in wastewater treatment, bio-fertilizer and soil remediation etc. However, their action mechanisms have not yet been well understood. We screened metal-tolerant bacteria isolated from the rhizosphere soil samples with metal-enriched media containing Cu, Fe or Mn, sequenced and compared the genomes, and analyzed their metal adaptation strategies at genomic levels to better understand their mechanisms of actions. Totally 32 metal-tolerant isolates were identified and classified into 12 genera based on phylogenetic analysis. The determination of MTC and effect of metal ions on the isolates indicated thatSerratia marcescensX1,Mammaliicoccus sciuriX26 andRummeliibacillus pycnusX33 showed the significant differences in metal tolerance to Cu, Fe and Mn with other isolates. They have quite different genomic features to adapt various metal ions.S. marcescensX1 possesses abundant genes required for Cu, Fe and Mn homeostasis.M. sciuriX26 has a number of genes involved in Mn and Zn homeostasis but with no genes responsible for Cu and Ca transport.R. pycnusX33 is rich in Fe, Zn and Mg transport systems but poor in Cu and Mn transport systems. It is thus inferred that the combined use of them would compensate their differences and enhance their ability in accumulating a wider range of heavy metals for promoting their applications in wastewater treatment, soil remediation and organic fertilizer etc.IMPORTANCEMetal-tolerant bacteria have wide applications in environment, agriculture and ecology, but their action strategies have not yet been well understood. We isolated 32 metal-tolerant bacteria from the rhizosphere soil samples. Among them,S. marcescensX1,M. sciuriX26 andR. pycnusX33 showed the significant differences in metal tolerance to Cu, Fe and Mn with other isolates. Comparative genomic analysis revealed that they have abundant and different genomic features to adapt various metal ions. It is thus inferred that the combined use of them would compensate their differences and enhance their ability in accumulating heavy metal ions widening their applications in industry, agriculture and ecology.

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Acid and Heavy Metal Tolerant Bacillus sp. from Rat-Hole Coal Mines of Meghalaya, India

Cited by 11 publications

References 13 publications

Exploring the application of biostimulation strategy for bacteria in the bioremediation of industrial effluent

Exploring the application of biostimulation strategy for bacteria in the bioremediation of industrial effluent

Effects of biochar and biofertilizer on cadmium-contaminated cotton growth and the antioxidative defense system

Comparative Genomic Analysis of Metal-Tolerant Bacteria Reveals Significant Differences in Metal Adaptation Strategies

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