Acid-Fast Intranuclear Inclusion Bodies in the Kidneys of Mallards Fed Lead Shot

Locke, Louis N.; Bagley, George E.; Irby, Harold D.

doi:10.7589/0090-3558-2.4.127

Cited by 44 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There was, however, no reason to suspect lead poisoning in these birds. Although acid fast intra-nuclear inclusions occur in the epithelial cells of the renal tubules in lead poisoning (Locke et al, 1966) they seldom occur in the liver (Keymer, unpublished) and are quite different from herpesvirus inclusions. It is possible that the infection arose in the swans due to the introduction of some Muscovy ducks about four weeks before the swans became ill, especially as these Downloaded by [University of Massachusetts] at 23:29 27 December 2014 ducks appear to be more susceptible to the infection than many other species (Hwang et al, 1975;Gough, 1984).…”

Section: Discussionmentioning

confidence: 95%

Duck virus enteritis (anatid herpesvirus infection) in mute swans(Cygnus Olor)

Keymer

Gough

1986

Avian Pathology

View full text Add to dashboard Cite

An outbreak of duck virus enteritis (DVE) in mute swans (Cygnus olor) is described. Ninety-nine captive swans in a flock of 440 died within 3 months. The origin was obscure. The infection did not spread to other species on the same premises. Clinical signs included polydypsia, photophobia, inappetance, drooping wings, watery nasal discharge and yellowish diarrhoea. Necropsies were made on eight swans. All showed a diphtheritic oesophagitis especially near the oesophageal-proventricular junction. Whitish, necrotic plaques from 1 mm to 3 cm in extent affected the epithelium of the small intestine. Other lesions were present but less consistent. Histological examination of livers showed typical lesions of DVE associated with intranuclear inclusions. The virus was propagated in duck kidney cell cultures and on second passage caused a cytopathic effect with intranuclear inclusions typical of DVE. Presence of virus was confirmed by electron microscopy. Experimentally the virus infected susceptible, unvaccinated ducklings, but not those vaccinated against DVE.

show abstract

Section: Discussionmentioning

confidence: 95%

Duck virus enteritis (anatid herpesvirus infection) in mute swans(Cygnus Olor)

Keymer

Gough

1986

Avian Pathology

View full text Add to dashboard Cite

show abstract

“…They have been found in many species of mammals (11,(13)(14)(15)(16), in birds (17,18), and in moss leaf cells (19). Although the inclusion bodies are almost always found in nuclei, they are sometimes found in the cytoplasm (16).…”

Section: Discussionmentioning

confidence: 99%

Lead-Induced Inclusion Bodies: Composition and Probable Role in Lead Metabolism

Moore¹,

Goyer²

1974

Environ Health Perspect

View full text Add to dashboard Cite

Lead-induced inclusion bodies in renal tubular cells of rats have been studied in vitro after isolation by differential centrifugation. The inclusion bodies are insoluble in physiological media but may be dissolved in denaturants like 6M urea and sodium deoxycholate. They contain about 40–50 μg of lead/mg protein, but only about 10% of this is tightly bound. They also contain calcium, iron, zinc, copper, and cadmium. The protein is rich in glutamic and aspartic acids, glycine and cystine. When dissolved in 6M urea, the protein migrates as a single band on acrylamide gel electrophoresis and has a molecular weight of 27,500. It is suggested that the inclusion bodies function as an intracellular depot of nondiffusible lead. Further studies have been directed toward finding a free, unaggregated lead-containing protein fraction. Nuclear proteins from kidneys of lead-toxic rats were separated into NaCl-, Tris-, and NaOH-soluble fractions and an insoluble acidic fraction. A quantitatively small lead-containing protein was found in the 0.14M NaCl fraction. Amino acid composition, electrophoretic mobility, molecular weight, and ability to bind lead are similar to those of insoluble inclusion body protein. The possible role of this soluble lead-binding protein in the formation of nuclear inclusion bodies is at present time not certain. These studies do suggest, however, that protein-bound lead in renal tubular cells may be partitioned between insoluble and nondiffusible morphologically discrete inclusion bodies and a soluble, extractable fraction which is presumably diffusable. ImagesFIGURE 1.FIGURE 2.FIGURE 3.

show abstract

“…Their formation seems to be a universal phenomena in most living things. They have been found in many species of mammals (11,(13)(14)(15)(16), in birds (17,18), and in moss leaf cells (19). Although the inclusion bodies are almost always found in nuclei, they are sometimes found in the cytoplasm (16).…”

Section: Figure 3 Polyacrylamide Gels (7%) Showing the Electrophoretmentioning

confidence: 98%