Acid Hydrolases in Monocytes From Patients With Inflammatory Bowel Disease, Chronic Liver Disease, and Rheumatoid Arthritis

Ganguly, Nilanjan; Lloyd, Brian L.; Price, C P; Kingham, J. G. C.; Lloyd, R. S.; Triger, D. R.; Wright, Ralph

doi:10.1016/s0140-6736(78)90917-0

Cited by 65 publications

(24 citation statements)

References 13 publications

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“…Normal intestinal microflora comprises an estimated 400 different bacterial species that reach their highest concentrations in the terminal ileum and colon [7,8] . Intestinal microflora produces toxic compounds, such as Gram-negative bacterial endotoxin, and harmful enzymes, such as β-glucuronidase and tryptophanase, which produce cytotoxic or carcinogenic agents [9][10][11] .…”

Section: Introductionmentioning

confidence: 99%

Effect of probiotics on pro-inflammatory cytokines and NF-κB activation in ulcerative colitis

Hegazy

El-Bedewy²

2010

WJG

236

128

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AIM:To demonstrate the therapeutic effect of probiotics in patients with ulcerative colitis (UC), and their effect on inflammatory mediators and nuclear factor (NF)-κB activation in these patients. METHODS:Thirty patients with mild to moderate UC were randomly classified into two groups: sulfasalazine group, who received sulfasalazine 2400 mg/d; and probiotic group, who received sulfasalazine 2400 mg/d with probiotic. The patients were investigated before and after 8 wk of treatment with probiotic (Lactobacillus delbruekii and Lactobacillus fermentum ). Colonic activity of myeloperoxidase (MPO) was assayed with UV spectrophotometry, the colonic content of interleukin (IL)-6 was determined by enzyme-linked immunosorbent assay (ELISA), fecal calprotectin was determined by ELISA, and expression of NF-κB p65 and tumor necrosis factor (TNF)-α proteins in colonic tissue was identified by immunohistochemistry and reverse transcription polymerase chain reaction, respectively. RESULTS:At the start of the study, colonic mucosal injury and inflammation were demonstrated in UC patients by hematoxylin and eosin staining, and an increase in colonic MPO activity, fecal calprotectin, and expression of colonic TNF-α and NF-κB p65 proteins. The use of probiotic for 8 wk significantly ameliorated the inflammation by decreasing the colonic concentration of IL-6, expression of TNF-α and NF-κB p65, leukocyte recruitment, as demonstrated by a decrease in colonic MPO activity, and the level of fecal calprotectin compared to sulfasalazine group and the control group (P < 0.05). CONCLUSION:Oral supplementation with probiotics could be helpful in maintaining remission and preventing relapse of UC.

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Section: Introductionmentioning

confidence: 99%

Effect of probiotics on pro-inflammatory cytokines and NF-κB activation in ulcerative colitis

Hegazy

El-Bedewy²

2010

WJG

236

128

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“…Although several groups of investigators reported elevated levels of glycosidases in rheumatic diseases in the 1970s (9)(10)(11), those studies were not pursued, and they have received little or no attention lately. The lack of interest is surprising in light of the fact that most cartilage matrix macromolecules are glycosylated, and some of them carry an extremely high amount of carbohydrates.…”

mentioning

confidence: 99%

Synovial fluid exoglycosidases are predictors of rheumatoid arthritis and are effective in cartilage glycosaminoglycan depletion

Ortutay

Polgar

Gömör³

et al. 2003

Arthritis & Rheumatism

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Objective. To analyze enzymes involved in joint damage by simultaneous investigation of glycosidases and matrix metalloproteinases (MMPs) in patients with various joint diseases. In joint diseases, the major clinical symptoms and disability of patients are caused by an irreversible destruction of hyaline cartilage. Enzymes capable of degrading extracellular matrix components (collagen and aggrecan) and concomitantly exposing chondrocytes to a variety of cytotoxic and/or apoptosis-inducing factors are considered to be the major effector molecules in cartilage degradation. Methods. Activities of glycosidases (␤-DRecently, significant advances have been made in our understanding of joint destruction and the mechanism of proteolytic cleavage of cartilage. Active proteases are currently implicated in the destructive processes and include matrix metalloproteinases (MMPs), the ADAM family (1), the ADAM-TS family (2), and serine proteases (elastase, cathepsins, and granzymes) (3-5). Of the 4 groups of MMPs, collagenase (MMP-1 in particular) appears to be responsible for the degradation of interstitial collagens. The gelatinases (including MMP-2 and MMP-9) degrade the denatured form of collagens, thus acting in synergy with MMP-1. The stromelysins (including MMP-3) have a broader substrate specificity for non-connective tissue components.

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“…Monocytes from patients with chronic hepatitis have shown high production and release of lysosomal enzymes, even without the stimulation of phagocytosis, suggesting that these cells had already been activated (20). Bradfield and Wells (10) indicated that Kupffer cells play an important role in the pathogenesis of hepatitis.…”

Section: Pma ~Horbolmvristate Acetate Pmn~; Polymor~honuclear Neutromentioning

confidence: 99%

A Mechanism for Liver Cell Injury in Viral Hepatitis: Effects of Hepatitis B Virus on Neutrophil Function in Vitro and in Children with Chronic Active Hepatitis

et al. 1983

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Acid Hydrolases in Monocytes From Patients With Inflammatory Bowel Disease, Chronic Liver Disease, and Rheumatoid Arthritis

Cited by 65 publications

References 13 publications

Effect of probiotics on pro-inflammatory cytokines and NF-κB activation in ulcerative colitis

Effect of probiotics on pro-inflammatory cytokines and NF-κB activation in ulcerative colitis

Synovial fluid exoglycosidases are predictors of rheumatoid arthritis and are effective in cartilage glycosaminoglycan depletion

A Mechanism for Liver Cell Injury in Viral Hepatitis: Effects of Hepatitis B Virus on Neutrophil Function in Vitro and in Children with Chronic Active Hepatitis

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